BRENDA - Enzyme Database

Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme

Van Rompay, A.R.; Johansson, M.; Karlsson, A.; Mol. Pharmacol. 56, 562-569 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.14
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.45
-
2',2'-difluorodeoxycytidine
pH 8.0, 37°C
Homo sapiens
2.7.4.14
0.5
-
CMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
1.4
-
araCMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
1.6
-
UMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
5.9
-
dUMP
pH 8.0, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytosol
-
Homo sapiens
5829
-
2.7.4.14
nucleus
-
Homo sapiens
5634
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
P30085
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 1-beta-D-arabinofuranosylcytosine
-
645156
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + 2',2'-difluorodeoxycytidine
-
645156
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + AMP
-
645156
Homo sapiens
ADP + ADP
-
-
-
r
2.7.4.14
ATP + ara-CMP
-
645156
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + CMP
-
645156
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dAMP
-
645156
Homo sapiens
ADP + dADP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645156
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
645156
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645156
Homo sapiens
ADP + UDP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.45
-
2',2'-difluorodeoxycytidine
pH 8.0, 37°C
Homo sapiens
2.7.4.14
0.5
-
CMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
1.4
-
araCMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
1.6
-
UMP
pH 8.0, 37°C
Homo sapiens
2.7.4.14
5.9
-
dUMP
pH 8.0, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.14
cytosol
-
Homo sapiens
5829
-
2.7.4.14
nucleus
-
Homo sapiens
5634
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 1-beta-D-arabinofuranosylcytosine
-
645156
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + 2',2'-difluorodeoxycytidine
-
645156
Homo sapiens
ADP + ?
-
-
-
?
2.7.4.14
ATP + AMP
-
645156
Homo sapiens
ADP + ADP
-
-
-
r
2.7.4.14
ATP + ara-CMP
-
645156
Homo sapiens
ADP + ara-CDP
-
-
-
?
2.7.4.14
ATP + CMP
-
645156
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dAMP
-
645156
Homo sapiens
ADP + dADP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645156
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
-
645156
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645156
Homo sapiens
ADP + UDP
-
-
-
?