EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.25 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli K-12 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.4.25 | hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20°C | Escherichia coli K-12 |
2.7.4.25 | hanging drop vapor diffusion technique | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.25 | V164E | substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase | Escherichia coli K-12 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.25 | additional information | TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme | Escherichia coli K-12 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.25 | 0.035 | - |
CMP | reaction with ATP | Escherichia coli | |
2.7.4.25 | 0.035 | - |
CMP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.038 | - |
ATP | pH 7.4, reaction with CMP | Escherichia coli | |
2.7.4.25 | 0.038 | - |
ATP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.087 | - |
dATP | pH 7.4, reaction with CMP | Escherichia coli | |
2.7.4.25 | 0.087 | - |
dATP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.094 | - |
dGMP | reaction with ATP | Escherichia coli | |
2.7.4.25 | 0.094 | - |
dCMP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.36 | - |
araCMP | reaction with ATP | Escherichia coli | |
2.7.4.25 | 0.36 | - |
1-beta-D-arabinofuranosylcytosine 5'-phosphate | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.64 | - |
GTP | reaction with CMP | Escherichia coli | |
2.7.4.25 | 0.64 | - |
GTP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.87 | - |
ITP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 0.93 | - |
UMP | reaction with ATP | Escherichia coli | |
2.7.4.25 | 0.93 | - |
UMP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 | |
2.7.4.25 | 1.46 | - |
dUMP | in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C | Escherichia coli K-12 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.25 | 24620 | - |
electrospray ionization mass spectrometry | Escherichia coli K-12 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.25 | Escherichia coli | - |
- |
- |
2.7.4.25 | Escherichia coli K-12 | P0A6I0 | strain NM554 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.25 | - |
Escherichia coli |
2.7.4.25 | blue Sepharose column chromatography and Ultrogel AcA54 gel filtration | Escherichia coli K-12 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.25 | ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
Escherichia coli K-12 | ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate | - |
? | |
2.7.4.25 | ATP + 2-thiouridine 5'-monophosphate | 2-thiouridine 5'-monophosphate is a poor substrate | Escherichia coli K-12 | ADP + 2-thiouridine 5'-diphosphate | - |
? | |
2.7.4.25 | ATP + ara-CMP | - |
Escherichia coli | ADP + ara-CDP | - |
? | |
2.7.4.25 | ATP + CMP | - |
Escherichia coli | ADP + CDP | - |
? | |
2.7.4.25 | ATP + CMP | - |
Escherichia coli K-12 | ADP + CDP | - |
? | |
2.7.4.25 | ATP + dCMP | - |
Escherichia coli | ADP + dCDP | - |
? | |
2.7.4.25 | ATP + dCMP | - |
Escherichia coli K-12 | ADP + dCDP | - |
? | |
2.7.4.25 | ATP + dGMP | - |
Escherichia coli | ADP + dGDP | - |
? | |
2.7.4.25 | ATP + dUMP | dUMP is a poor substrate | Escherichia coli K-12 | ADP + dUDP | - |
? | |
2.7.4.25 | ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
? | |
2.7.4.25 | ATP + UMP | UMP is a poor substrate | Escherichia coli K-12 | ADP + UDP | - |
? | |
2.7.4.25 | CTP + CMP | the activity with CTP is still measurable but less than 0.05% of that with ATP | Escherichia coli K-12 | CDP + CDP | - |
? | |
2.7.4.25 | dATP + CMP | - |
Escherichia coli | dADP + CDP | - |
? | |
2.7.4.25 | dATP + CMP | - |
Escherichia coli K-12 | dADP + CDP | - |
? | |
2.7.4.25 | dCTP + CMP | the activity with dCTP is still measurable but less than 0.05% of that with ATP | Escherichia coli K-12 | dCDP + CDP | - |
? | |
2.7.4.25 | dUTP + CMP | the activity with dUTP is still measurable but less than 0.05% of that with ATP | Escherichia coli K-12 | dUDP + CDP | - |
? | |
2.7.4.25 | GTP + CMP | - |
Escherichia coli | GDP + CDP | - |
? | |
2.7.4.25 | GTP + CMP | - |
Escherichia coli K-12 | GDP + CDP | - |
? | |
2.7.4.25 | ITP + CMP | ITP is a poor substrate | Escherichia coli K-12 | IDP + CDP | - |
? | |
2.7.4.25 | additional information | TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates | Escherichia coli K-12 | ? | - |
? | |
2.7.4.25 | UTP + CMP | the activity with UTP is still measurable but less than 0.05% of that with ATP | Escherichia coli K-12 | UDP + CDP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.25 | monomer | 2 * 000, SDS-PAGE | Escherichia coli K-12 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.25 | CMP kinase | - |
Escherichia coli K-12 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.25 | 52 | - |
CMP kinase is half-inactivated at 52°C | Escherichia coli K-12 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.25 | ATP | - |
Escherichia coli K-12 |