BRENDA - Enzyme Database

CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases

Bucurenci, N.; Sakamoto, H.; Briozzo, P.; Palibroda, N.; Serina, L.; Sarfati, R.S.; Labesse, G.; Briand, G.; Danchin, A.; Barzu, O.; Gilles, A.M.; J. Biol. Chem. 271, 2856-2862 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli K-12
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.4.25
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20C
Escherichia coli K-12
2.7.4.25
hanging drop vapor diffusion technique
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.25
V164E
substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase
Escherichia coli K-12
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.25
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme
Escherichia coli K-12
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.035
-
CMP
reaction with ATP
Escherichia coli
2.7.4.25
0.035
-
CMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.038
-
ATP
pH 7.4, reaction with CMP
Escherichia coli
2.7.4.25
0.038
-
ATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.087
-
dATP
pH 7.4, reaction with CMP
Escherichia coli
2.7.4.25
0.087
-
dATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.094
-
dCMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.094
-
dGMP
reaction with ATP
Escherichia coli
2.7.4.25
0.36
-
1-beta-D-arabinofuranosylcytosine 5'-phosphate
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.36
-
araCMP
reaction with ATP
Escherichia coli
2.7.4.25
0.64
-
GTP
reaction with CMP
Escherichia coli
2.7.4.25
0.64
-
GTP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.87
-
ITP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.93
-
UMP
reaction with ATP
Escherichia coli
2.7.4.25
0.93
-
UMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
1.46
-
dUMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.25
24620
-
electrospray ionization mass spectrometry
Escherichia coli K-12
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.25
Escherichia coli
-
-
-
2.7.4.25
Escherichia coli K-12
P0A6I0
strain NM554
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.25
-
Escherichia coli
2.7.4.25
blue Sepharose column chromatography and Ultrogel AcA54 gel filtration
Escherichia coli K-12
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
645154
Escherichia coli K-12
ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate
-
-
-
?
2.7.4.25
ATP + 2-thiouridine 5'-monophosphate
2-thiouridine 5'-monophosphate is a poor substrate
645154
Escherichia coli K-12
ADP + 2-thiouridine 5'-diphosphate
-
-
-
?
2.7.4.25
ATP + ara-CMP
-
645154
Escherichia coli
ADP + ara-CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645154
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645154
Escherichia coli K-12
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645154
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645154
Escherichia coli K-12
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dUMP
dUMP is a poor substrate
645154
Escherichia coli K-12
ADP + dUDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645154
Escherichia coli
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
645154
Escherichia coli K-12
ADP + UDP
-
-
-
?
2.7.4.25
CTP + CMP
the activity with CTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
CDP + CDP
-
-
-
?
2.7.4.25
dATP + CMP
-
645154
Escherichia coli
dADP + CDP
-
-
-
-
2.7.4.25
dATP + CMP
-
645154
Escherichia coli K-12
dADP + CDP
-
-
-
?
2.7.4.25
dCTP + CMP
the activity with dCTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dCDP + CDP
-
-
-
?
2.7.4.25
dUTP + CMP
the activity with dUTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dUDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
-
645154
Escherichia coli
GDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
-
645154
Escherichia coli K-12
GDP + CDP
-
-
-
?
2.7.4.25
ITP + CMP
ITP is a poor substrate
645154
Escherichia coli K-12
IDP + CDP
-
-
-
?
2.7.4.25
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates
645154
Escherichia coli K-12
?
-
-
-
-
2.7.4.25
UTP + CMP
the activity with UTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
UDP + CDP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.4.25
monomer
2 * 000, SDS-PAGE
Escherichia coli K-12
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
52
-
CMP kinase is half-inactivated at 52C
Escherichia coli K-12
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Escherichia coli K-12
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli K-12
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Escherichia coli K-12
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.4.25
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20C
Escherichia coli K-12
2.7.4.25
hanging drop vapor diffusion technique
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.4.25
V164E
substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase
Escherichia coli K-12
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.25
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme
Escherichia coli K-12
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.035
-
CMP
reaction with ATP
Escherichia coli
2.7.4.25
0.035
-
CMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.038
-
ATP
pH 7.4, reaction with CMP
Escherichia coli
2.7.4.25
0.038
-
ATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.087
-
dATP
pH 7.4, reaction with CMP
Escherichia coli
2.7.4.25
0.087
-
dATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.094
-
dCMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.094
-
dGMP
reaction with ATP
Escherichia coli
2.7.4.25
0.36
-
1-beta-D-arabinofuranosylcytosine 5'-phosphate
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.36
-
araCMP
reaction with ATP
Escherichia coli
2.7.4.25
0.64
-
GTP
reaction with CMP
Escherichia coli
2.7.4.25
0.64
-
GTP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.87
-
ITP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
0.93
-
UMP
reaction with ATP
Escherichia coli
2.7.4.25
0.93
-
UMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
2.7.4.25
1.46
-
dUMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.25
24620
-
electrospray ionization mass spectrometry
Escherichia coli K-12
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
-
Escherichia coli
2.7.4.25
blue Sepharose column chromatography and Ultrogel AcA54 gel filtration
Escherichia coli K-12
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
645154
Escherichia coli K-12
ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate
-
-
-
?
2.7.4.25
ATP + 2-thiouridine 5'-monophosphate
2-thiouridine 5'-monophosphate is a poor substrate
645154
Escherichia coli K-12
ADP + 2-thiouridine 5'-diphosphate
-
-
-
?
2.7.4.25
ATP + ara-CMP
-
645154
Escherichia coli
ADP + ara-CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645154
Escherichia coli
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
-
645154
Escherichia coli K-12
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645154
Escherichia coli
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
645154
Escherichia coli K-12
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dUMP
dUMP is a poor substrate
645154
Escherichia coli K-12
ADP + dUDP
-
-
-
?
2.7.4.25
ATP + UMP
-
645154
Escherichia coli
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
645154
Escherichia coli K-12
ADP + UDP
-
-
-
?
2.7.4.25
CTP + CMP
the activity with CTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
CDP + CDP
-
-
-
?
2.7.4.25
dATP + CMP
-
645154
Escherichia coli
dADP + CDP
-
-
-
-
2.7.4.25
dATP + CMP
-
645154
Escherichia coli K-12
dADP + CDP
-
-
-
?
2.7.4.25
dCTP + CMP
the activity with dCTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dCDP + CDP
-
-
-
?
2.7.4.25
dUTP + CMP
the activity with dUTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dUDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
-
645154
Escherichia coli
GDP + CDP
-
-
-
?
2.7.4.25
GTP + CMP
-
645154
Escherichia coli K-12
GDP + CDP
-
-
-
?
2.7.4.25
ITP + CMP
ITP is a poor substrate
645154
Escherichia coli K-12
IDP + CDP
-
-
-
?
2.7.4.25
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates
645154
Escherichia coli K-12
?
-
-
-
-
2.7.4.25
UTP + CMP
the activity with UTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
UDP + CDP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.4.25
monomer
2 * 000, SDS-PAGE
Escherichia coli K-12
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.4.25
52
-
CMP kinase is half-inactivated at 52C
Escherichia coli K-12