BRENDA - Enzyme Database

Purification and properties of pyrimidine nucleoside monophosphate kinase from baker‘s yeast

Kohno, H.; Kumagai, H.; Tochikura, T.; Agric. Biol. Chem. 47, 19-24 (1983)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
dithiothreitol
1 mM, activates; activates
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
competitive inhibition of UMP phosphorylation
Saccharomyces cerevisiae
2.7.4.14
dCMP
competitive inhibition of UMP phosphorylation
Saccharomyces cerevisiae
2.7.4.14
HgCl2
0.1 mM, 88% inhibition
Saccharomyces cerevisiae
2.7.4.14
NEM
1.0 mM; 41% inhibition
Saccharomyces cerevisiae
2.7.4.14
PCMB
0.1 mM, 69% loss of activity
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.052
-
UMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
0.071
-
CMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
0.54
-
dCMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
8.5
-
dUMP
pH 7.5, 25°C
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Co2+
divalent cation required, Mn2+ can substitute for Mg2+
Saccharomyces cerevisiae
2.7.4.14
Mg2+
divalent cation required, Mg2+ is most effective
Saccharomyces cerevisiae
2.7.4.14
Mn2+
divalent cation required, Mn2+ can substitute for Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.14
26000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
11.1
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645149
Saccharomyces cerevisiae
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645149
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645149
Saccharomyces cerevisiae
ADP + UDP
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.14
4
-
at pH 7 the enzyme is most stable when kept at 4°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
7.5
-
reaction with UMP and ATP
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.14
4
9
pH 4.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, reaction with UMP and ATP
Saccharomyces cerevisiae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.4.14
7
-
at pH 7.0, the enzyme is most stable when kept at 4°C
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
dithiothreitol
1 mM, activates; activates
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
competitive inhibition of UMP phosphorylation
Saccharomyces cerevisiae
2.7.4.14
dCMP
competitive inhibition of UMP phosphorylation
Saccharomyces cerevisiae
2.7.4.14
HgCl2
0.1 mM, 88% inhibition
Saccharomyces cerevisiae
2.7.4.14
NEM
1.0 mM; 41% inhibition
Saccharomyces cerevisiae
2.7.4.14
PCMB
0.1 mM, 69% loss of activity
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.052
-
UMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
0.071
-
CMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
0.54
-
dCMP
pH 7.5, 25°C
Saccharomyces cerevisiae
2.7.4.14
8.5
-
dUMP
pH 7.5, 25°C
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Co2+
divalent cation required, Mn2+ can substitute for Mg2+
Saccharomyces cerevisiae
2.7.4.14
Mg2+
divalent cation required, Mg2+ is most effective
Saccharomyces cerevisiae
2.7.4.14
Mn2+
divalent cation required, Mn2+ can substitute for Mg2+
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.14
26000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
11.1
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645149
Saccharomyces cerevisiae
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
-
645149
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + UMP
-
645149
Saccharomyces cerevisiae
ADP + UDP
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.14
4
-
at pH 7 the enzyme is most stable when kept at 4°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
7.5
-
reaction with UMP and ATP
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.4.14
4
9
pH 4.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, reaction with UMP and ATP
Saccharomyces cerevisiae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.4.14
7
-
at pH 7.0, the enzyme is most stable when kept at 4°C
Saccharomyces cerevisiae