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Literature summary extracted from

  • Maness, P.; Orengo, A.
    A pyrimidine nucleoside monophosphate kinase from rat liver (1975), Biochemistry, 14, 1484-1489.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.14 2-mercaptoethanol activates Rattus norvegicus
2.7.4.14 dithiothreitol activates Rattus norvegicus
2.7.4.14 glutathione activates Rattus norvegicus
2.7.4.14 L-Cys activates Rattus norvegicus
2.7.4.14 reduced DL-alpha-lipoic acid activates Rattus norvegicus
2.7.4.14 thioredoxin activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 CMP above 0.13 mM, substrate inhibition Rattus norvegicus
2.7.4.14 additional information no substrate inhibition with 3 mM dCMP and 1 mM UMP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.027
-
 dCMP
-
 Rattus norvegicus
2.7.4.14 0.03
-
 CMP
-
 Rattus norvegicus
2.7.4.14 0.04
-
 UMP
-
 Rattus norvegicus
2.7.4.14 0.053
-
 UMP
-
 Rattus norvegicus
2.7.4.14 0.98
-
 CMP
-
 Rattus norvegicus
2.7.4.14 2.77
-
 dCMP
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Mg2+ required for phosphorylation of CMP, UMP and dCMP by either ATP or dCTP Rattus norvegicus
2.7.4.14 Mn2+ with CMP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+, 100%, Mn2+, 42%, Ni2+, 15% and Ca2+, 13% Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.14 30.17
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + CMP
-
 Rattus norvegicus ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP
-
 Rattus norvegicus ADP + dCDP
-
 ?
2.7.4.14 ATP + UMP
-
 Rattus norvegicus ADP + UDP
-
 ?
2.7.4.14 CTP + CMP
-
 Rattus norvegicus CDP + CDP
-
 ?
2.7.4.14 dATP + CMP
-
 Rattus norvegicus dADP + CDP
-
 ?
2.7.4.14 dATP + dCMP
-
 Rattus norvegicus dADP + dCDP
-
 ?
2.7.4.14 dATP + UMP
-
 Rattus norvegicus dADP + ADP
-
 ?
2.7.4.14 dCTP + CMP
-
 Rattus norvegicus dCDP + CDP
-
 ?
2.7.4.14 dGTP + CMP
-
 Rattus norvegicus dGDP + CDP
-
 ?
2.7.4.14 dTTP + CMP
-
 Rattus norvegicus dTDP + CDP
-
 ?
2.7.4.14 dUTP + CMP
-
 Rattus norvegicus dUDP + CDP
-
 ?
2.7.4.14 GTP + CMP
-
 Rattus norvegicus GDP + CDP
-
 ?
2.7.4.14 ITP + CMP
-
 Rattus norvegicus IDP + CDP
-
 ?
2.7.4.14 ITP + UMP
-
 Rattus norvegicus IDP + UDP
-
 ?
2.7.4.14 UTP + CMP
-
 Rattus norvegicus UDP + CDP
-
 ?
2.7.4.14 XTP + CMP
-
 Rattus norvegicus XDP + CDP
-
 ?