BRENDA - Enzyme Database

A pyrimidine nucleoside monophosphate kinase from rat liver

Maness, P.; Orengo, A.; Biochemistry 14, 1484-1489 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
2-mercaptoethanol
activates
Rattus norvegicus
2.7.4.14
dithiothreitol
activates
Rattus norvegicus
2.7.4.14
glutathione
activates
Rattus norvegicus
2.7.4.14
L-Cys
activates
Rattus norvegicus
2.7.4.14
reduced DL-alpha-lipoic acid
activates
Rattus norvegicus
2.7.4.14
thioredoxin
activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
above 0.13 mM, substrate inhibition
Rattus norvegicus
2.7.4.14
additional information
no substrate inhibition with 3 mM dCMP and 1 mM UMP
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.027
-
dCMP
-
Rattus norvegicus
2.7.4.14
0.03
-
CMP
-
Rattus norvegicus
2.7.4.14
0.04
-
UMP
-
Rattus norvegicus
2.7.4.14
0.053
-
UMP
-
Rattus norvegicus
2.7.4.14
0.98
-
CMP
-
Rattus norvegicus
2.7.4.14
2.77
-
dCMP
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required for phosphorylation of CMP, UMP and dCMP by either ATP or dCTP
Rattus norvegicus
2.7.4.14
Mn2+
with CMP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+, 100%, Mn2+, 42%, Ni2+, 15% and Ca2+, 13%
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
30.17
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645143
Rattus norvegicus
ADP + CDP
-
645143
Rattus norvegicus
?
2.7.4.14
ATP + dCMP
-
645143
Rattus norvegicus
ADP + dCDP
-
645143
Rattus norvegicus
?
2.7.4.14
ATP + UMP
-
645143
Rattus norvegicus
ADP + UDP
-
645143
Rattus norvegicus
?
2.7.4.14
CTP + CMP
-
645143
Rattus norvegicus
CDP + CDP
-
-
-
?
2.7.4.14
dATP + CMP
-
645143
Rattus norvegicus
dADP + CDP
-
-
-
?
2.7.4.14
dATP + dCMP
-
645143
Rattus norvegicus
dADP + dCDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645143
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + CMP
-
645143
Rattus norvegicus
dCDP + CDP
-
-
-
?
2.7.4.14
dGTP + CMP
-
645143
Rattus norvegicus
dGDP + CDP
-
-
-
?
2.7.4.14
dTTP + CMP
-
645143
Rattus norvegicus
dTDP + CDP
-
-
-
?
2.7.4.14
dUTP + CMP
-
645143
Rattus norvegicus
dUDP + CDP
-
-
-
?
2.7.4.14
GTP + CMP
-
645143
Rattus norvegicus
GDP + CDP
-
-
-
?
2.7.4.14
ITP + CMP
-
645143
Rattus norvegicus
IDP + CDP
-
-
-
?
2.7.4.14
ITP + UMP
-
645143
Rattus norvegicus
IDP + UDP
-
-
-
?
2.7.4.14
UTP + CMP
-
645143
Rattus norvegicus
UDP + CDP
-
-
-
?
2.7.4.14
XTP + CMP
-
645143
Rattus norvegicus
XDP + CDP
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
2-mercaptoethanol
activates
Rattus norvegicus
2.7.4.14
dithiothreitol
activates
Rattus norvegicus
2.7.4.14
glutathione
activates
Rattus norvegicus
2.7.4.14
L-Cys
activates
Rattus norvegicus
2.7.4.14
reduced DL-alpha-lipoic acid
activates
Rattus norvegicus
2.7.4.14
thioredoxin
activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
above 0.13 mM, substrate inhibition
Rattus norvegicus
2.7.4.14
additional information
no substrate inhibition with 3 mM dCMP and 1 mM UMP
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.027
-
dCMP
-
Rattus norvegicus
2.7.4.14
0.03
-
CMP
-
Rattus norvegicus
2.7.4.14
0.04
-
UMP
-
Rattus norvegicus
2.7.4.14
0.053
-
UMP
-
Rattus norvegicus
2.7.4.14
0.98
-
CMP
-
Rattus norvegicus
2.7.4.14
2.77
-
dCMP
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
required for phosphorylation of CMP, UMP and dCMP by either ATP or dCTP
Rattus norvegicus
2.7.4.14
Mn2+
with CMP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+, 100%, Mn2+, 42%, Ni2+, 15% and Ca2+, 13%
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
30.17
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645143
Rattus norvegicus
ADP + CDP
-
645143
Rattus norvegicus
?
2.7.4.14
ATP + dCMP
-
645143
Rattus norvegicus
ADP + dCDP
-
645143
Rattus norvegicus
?
2.7.4.14
ATP + UMP
-
645143
Rattus norvegicus
ADP + UDP
-
645143
Rattus norvegicus
?
2.7.4.14
CTP + CMP
-
645143
Rattus norvegicus
CDP + CDP
-
-
-
?
2.7.4.14
dATP + CMP
-
645143
Rattus norvegicus
dADP + CDP
-
-
-
?
2.7.4.14
dATP + dCMP
-
645143
Rattus norvegicus
dADP + dCDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645143
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + CMP
-
645143
Rattus norvegicus
dCDP + CDP
-
-
-
?
2.7.4.14
dGTP + CMP
-
645143
Rattus norvegicus
dGDP + CDP
-
-
-
?
2.7.4.14
dTTP + CMP
-
645143
Rattus norvegicus
dTDP + CDP
-
-
-
?
2.7.4.14
dUTP + CMP
-
645143
Rattus norvegicus
dUDP + CDP
-
-
-
?
2.7.4.14
GTP + CMP
-
645143
Rattus norvegicus
GDP + CDP
-
-
-
?
2.7.4.14
ITP + CMP
-
645143
Rattus norvegicus
IDP + CDP
-
-
-
?
2.7.4.14
ITP + UMP
-
645143
Rattus norvegicus
IDP + UDP
-
-
-
?
2.7.4.14
UTP + CMP
-
645143
Rattus norvegicus
UDP + CDP
-
-
-
?
2.7.4.14
XTP + CMP
-
645143
Rattus norvegicus
XDP + CDP
-
-
-
?