BRENDA - Enzyme Database

Pyrimidine nucleoside monophosphate kinase from rat liver and rat Novikoff ascites hepatoma (EC 2.7.4.14)

Orengo, A.; Maness, P.; Methods Enzymol. 51, 321-331 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
thioredoxin
activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
above 0.13 mM, substrate inhibition
Rattus norvegicus
2.7.4.14
F-
complete inhibition at 25 mM
Rattus norvegicus
2.7.4.14
additional information
no substrate inhibition with 3 mM dCMP and 1 mM UMP
Rattus norvegicus
2.7.4.14
NaClO
250 mM
Rattus norvegicus
2.7.4.14
NaSCN
250 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.0053
-
CMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.027
-
dCMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.03
-
CMP
reaction with ATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.04
-
UMP
reaction with ATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.043
-
UMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.053
-
UMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.074
-
dATP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.134
-
ATP
reaction with dCMP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.32
-
ATP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.42
-
dATP
reaction with UMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.58
-
ATP
reaction with UMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.61
-
dATP
reaction with dCMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.68
-
ATP
reaction with dCMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.715
-
dCMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.82
-
dCTP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.98
-
CMP
reaction with dCTP, liver enzyme
Rattus norvegicus
2.7.4.14
1.1
-
dCMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
2.77
-
dCMP
reaction with ATP, liver enzyme
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Ca2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Mg2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Mn2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Na2SO4
250 mM, stimulates
Rattus norvegicus
2.7.4.14
NaCl
250 mM, stimulates
Rattus norvegicus
2.7.4.14
Ni2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.14
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
liver
-
Rattus norvegicus
-
2.7.4.14
Novikoff ascites hepatoma cell
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
4.2
-
reaction with CMP and ATP
Rattus norvegicus
2.7.4.14
4.98
-
reaction with dCMP and ATP
Rattus norvegicus
2.7.4.14
8.76
-
reaction with UMP and dCMP
Rattus norvegicus
2.7.4.14
30
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.7.4.14
-20°C, in 25 mM Tris-acetate buffer, pH 7.5, 50 mM 2-mercaptoethanol, 50% glycerol, stable for at least 2 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645139
Rattus norvegicus
ADP + CDP
-
645139
Rattus norvegicus
?
2.7.4.14
ATP + dCMP
-
645139
Rattus norvegicus
ADP + dCDP
-
645139
Rattus norvegicus
?
2.7.4.14
ATP + UMP
-
645139
Rattus norvegicus
ADP + UDP
-
645139
Rattus norvegicus
?
2.7.4.14
dATP + CMP
-
645139
Rattus norvegicus
dADP + CDP
-
-
-
?
2.7.4.14
dATP + dCMP
-
645139
Rattus norvegicus
dADP + dCDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645139
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + CMP
-
645139
Rattus norvegicus
dCDP + CDP
-
-
-
?
2.7.4.14
ITP + UMP
-
645139
Rattus norvegicus
IDP + UDP
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.14
thioredoxin
activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.14
CMP
above 0.13 mM, substrate inhibition
Rattus norvegicus
2.7.4.14
F-
complete inhibition at 25 mM
Rattus norvegicus
2.7.4.14
additional information
no substrate inhibition with 3 mM dCMP and 1 mM UMP
Rattus norvegicus
2.7.4.14
NaClO
250 mM
Rattus norvegicus
2.7.4.14
NaSCN
250 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.0053
-
CMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.027
-
dCMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.03
-
CMP
reaction with ATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.04
-
UMP
reaction with ATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.043
-
UMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.053
-
UMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
0.074
-
dATP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.134
-
ATP
reaction with dCMP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.32
-
ATP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.42
-
dATP
reaction with UMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.58
-
ATP
reaction with UMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.61
-
dATP
reaction with dCMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.68
-
ATP
reaction with dCMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.715
-
dCMP
reaction with ATP, enzyme from Novikoff ascites hepatoma
Rattus norvegicus
2.7.4.14
0.82
-
dCTP
reaction with CMP, liver enzyme
Rattus norvegicus
2.7.4.14
0.98
-
CMP
reaction with dCTP, liver enzyme
Rattus norvegicus
2.7.4.14
1.1
-
dCMP
reaction with dATP, liver enzyme
Rattus norvegicus
2.7.4.14
2.77
-
dCMP
reaction with ATP, liver enzyme
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Ca2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Mg2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Mn2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
2.7.4.14
Na2SO4
250 mM, stimulates
Rattus norvegicus
2.7.4.14
NaCl
250 mM, stimulates
Rattus norvegicus
2.7.4.14
Ni2+
required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%)
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.14
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.14
liver
-
Rattus norvegicus
-
2.7.4.14
Novikoff ascites hepatoma cell
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.14
4.2
-
reaction with CMP and ATP
Rattus norvegicus
2.7.4.14
4.98
-
reaction with dCMP and ATP
Rattus norvegicus
2.7.4.14
8.76
-
reaction with UMP and dCMP
Rattus norvegicus
2.7.4.14
30
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.4.14
-20°C, in 25 mM Tris-acetate buffer, pH 7.5, 50 mM 2-mercaptoethanol, 50% glycerol, stable for at least 2 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + CMP
-
645139
Rattus norvegicus
ADP + CDP
-
645139
Rattus norvegicus
?
2.7.4.14
ATP + dCMP
-
645139
Rattus norvegicus
ADP + dCDP
-
645139
Rattus norvegicus
?
2.7.4.14
ATP + UMP
-
645139
Rattus norvegicus
ADP + UDP
-
645139
Rattus norvegicus
?
2.7.4.14
dATP + CMP
-
645139
Rattus norvegicus
dADP + CDP
-
-
-
?
2.7.4.14
dATP + dCMP
-
645139
Rattus norvegicus
dADP + dCDP
-
-
-
?
2.7.4.14
dATP + UMP
-
645139
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.14
dCTP + CMP
-
645139
Rattus norvegicus
dCDP + CDP
-
-
-
?
2.7.4.14
ITP + UMP
-
645139
Rattus norvegicus
IDP + UDP
-
-
-
?