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Literature summary extracted from

  • Orengo, A.; Maness, P.
    Pyrimidine nucleoside monophosphate kinase from rat liver and rat Novikoff ascites hepatoma (EC 2.7.4.14) (1978), Methods Enzymol., 51, 321-331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.14 thioredoxin activity is strictly dependent upon sulfhydryl reducing agents. Reduced thioredoxin is by far the most effective Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 CMP above 0.13 mM, substrate inhibition Rattus norvegicus
2.7.4.14 F- complete inhibition at 25 mM Rattus norvegicus
2.7.4.14 additional information no substrate inhibition with 3 mM dCMP and 1 mM UMP Rattus norvegicus
2.7.4.14 NaClO 250 mM Rattus norvegicus
2.7.4.14 NaSCN 250 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.0053
-
 CMP reaction with ATP, enzyme from Novikoff ascites hepatoma Rattus norvegicus
2.7.4.14 0.027
-
 dCMP reaction with dATP, liver enzyme Rattus norvegicus
2.7.4.14 0.03
-
 CMP reaction with ATP, liver enzyme Rattus norvegicus
2.7.4.14 0.04
-
 UMP reaction with ATP, liver enzyme Rattus norvegicus
2.7.4.14 0.043
-
 UMP reaction with ATP, enzyme from Novikoff ascites hepatoma Rattus norvegicus
2.7.4.14 0.053
-
 UMP reaction with dATP, liver enzyme Rattus norvegicus
2.7.4.14 0.074
-
 dATP reaction with CMP, liver enzyme Rattus norvegicus
2.7.4.14 0.134
-
 ATP reaction with dCMP, enzyme from Novikoff ascites hepatoma Rattus norvegicus
2.7.4.14 0.32
-
 ATP reaction with CMP, liver enzyme Rattus norvegicus
2.7.4.14 0.42
-
 dATP reaction with UMP, liver enzyme Rattus norvegicus
2.7.4.14 0.58
-
 ATP reaction with UMP, liver enzyme Rattus norvegicus
2.7.4.14 0.61
-
 dATP reaction with dCMP, liver enzyme Rattus norvegicus
2.7.4.14 0.68
-
 ATP reaction with dCMP, liver enzyme Rattus norvegicus
2.7.4.14 0.715
-
 dCMP reaction with ATP, enzyme from Novikoff ascites hepatoma Rattus norvegicus
2.7.4.14 0.82
-
 dCTP reaction with CMP, liver enzyme Rattus norvegicus
2.7.4.14 0.98
-
 CMP reaction with dCTP, liver enzyme Rattus norvegicus
2.7.4.14 1.1
-
 dCMP reaction with dATP, liver enzyme Rattus norvegicus
2.7.4.14 2.77
-
 dCMP reaction with ATP, liver enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Ca2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) Rattus norvegicus
2.7.4.14 Mg2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) Rattus norvegicus
2.7.4.14 Mn2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) Rattus norvegicus
2.7.4.14 Na2SO4 250 mM, stimulates Rattus norvegicus
2.7.4.14 NaCl 250 mM, stimulates Rattus norvegicus
2.7.4.14 Ni2+ required for the phosphorylation of CMP, IUMP and dCMP by either ATP or dCTP. With CMP as phosphate acceptor and ATP as phosphate donor, Mn2+, Ni2+ and Ca2+ are able to substitute for Mg2+ but are less effective. The relative rates are Mg2+ (100%), Mn2+ (42%), Ni2+ (16%), and Ca2+ (13%) Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 liver
-
 Rattus norvegicus
-
2.7.4.14 Novikoff ascites hepatoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.14 4.2
-
 reaction with CMP and ATP Rattus norvegicus
2.7.4.14 4.98
-
 reaction with dCMP and ATP Rattus norvegicus
2.7.4.14 8.76
-
 reaction with UMP and dCMP Rattus norvegicus
2.7.4.14 30
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.4.14 -20°C, in 25 mM Tris-acetate buffer, pH 7.5, 50 mM 2-mercaptoethanol, 50% glycerol, stable for at least 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + CMP
-
 Rattus norvegicus ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP
-
 Rattus norvegicus ADP + dCDP
-
 ?
2.7.4.14 ATP + UMP
-
 Rattus norvegicus ADP + UDP
-
 ?
2.7.4.14 dATP + CMP
-
 Rattus norvegicus dADP + CDP
-
 ?
2.7.4.14 dATP + dCMP
-
 Rattus norvegicus dADP + dCDP
-
 ?
2.7.4.14 dATP + UMP
-
 Rattus norvegicus dADP + ADP
-
 ?
2.7.4.14 dCTP + CMP
-
 Rattus norvegicus dCDP + CDP
-
 ?
2.7.4.14 ITP + UMP
-
 Rattus norvegicus IDP + UDP
-
 ?