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Literature summary extracted from

  • Li, H.C.; Brown, G.G.
    Orthophosphate and histone dependent polyphosphate kinase from E. coli (1973), Biochem. Biophys. Res. Commun., 53, 875-881.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.1 basic protein activation Escherichia coli
2.7.4.1 bovine serum albumin activation, can substitute for histone only in the absence of phosphate Escherichia coli
2.7.4.1 casein activation, can substitute for histone only in the absence of phosphate Escherichia coli
2.7.4.1 histone strong inhibition of reverse reaction Escherichia coli
2.7.4.1 histone maximum activation in the presence of 20 mM phosphate Escherichia coli
2.7.4.1 phosphate inhibiton above 20 mM Escherichia coli
2.7.4.1 phosphate required for activity, only in the presence of histone Escherichia coli
2.7.4.1 protamine required for maximal activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 ADP 0.2 mM, complete inhibition Escherichia coli
2.7.4.1 diphosphate 1 mM, complete inhibition Escherichia coli
2.7.4.1 F- 20 mM, complete inhibition Escherichia coli
2.7.4.1 histone reverse reaction, strong, activates forward reaction in the presence of phosphate Escherichia coli
2.7.4.1 KCl
-
 Escherichia coli
2.7.4.1 additional information not inhibited by 3',5'-adenosine monophosphate Escherichia coli
2.7.4.1 NaCl
-
 Escherichia coli
2.7.4.1 phosphate above 20 mM; activates in the presence of histone Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 1.4
-
 ATP pH 7.0, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ activation Escherichia coli
2.7.4.1 Mn2+ activation, can replace Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Escherichia coli involved in phosphate metabolism of bacteria ADP + (phosphate)n+1
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Escherichia coli
-
 strain K-12, Crooke's strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 ammonium sulfate, DEAE-cellulose, Sephadex G-200 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 0.15
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Escherichia coli ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n involved in phosphate metabolism of bacteria Escherichia coli ADP + (phosphate)n+1
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 7
-
-
 Escherichia coli