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Literature summary extracted from

  • Doi, O.; Ogura, M.; Tanaka, N.; Umezawa, H.
    Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginoa (1968), Appl. Microbiol., 16, 1276-1281.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.95 soluble
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.95 Mg2+ activation Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.95 Pseudomonas aeruginosa
-
 ten strains: H1-H9 and A3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.95 ammonium sulfate, Sephadex G 50, Sephadex G 100, partial purification Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.95 ATP + kanamycin specific for ATP Pseudomonas aeruginosa ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin no activity with ADP Pseudomonas aeruginosa ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin phosphorylates the C-3 hydroxyl group of 6-amino-6-deoxy-D-glucose moiety of kanamycin B or C Pseudomonas aeruginosa ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + kanamycin no activity with streptomycin Pseudomonas aeruginosa ADP + kanamycin 3'-phosphate
-
 ?
2.7.1.95 ATP + neamine
-
 Pseudomonas aeruginosa ADP + neamine phosphate
-
 ?
2.7.1.95 ATP + neomycin
-
 Pseudomonas aeruginosa ADP + neomyin phosphate
-
 ?
2.7.1.95 ATP + paromomycin
-
 Pseudomonas aeruginosa ADP + paromomycin phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.95 35 37 assay at Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.95 6.5 8 approx. half-maximal activity at pH 6.5 and pH 8.0 Pseudomonas aeruginosa