EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.17 | 0.09 | - |
xylulose | pH 8.0, 25°C | Parageobacillus caldoxylosilyticus | |
2.7.1.17 | 0.16 | - |
ATP | pH 8.0, 25°C | Parageobacillus caldoxylosilyticus | |
2.7.1.17 | 0.16 | - |
ATP | ATP in form of MgATP2- | Parageobacillus caldoxylosilyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 54000 | - |
2 * 54000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
2.7.1.17 | 110000 | 115000 | gel filtration | Parageobacillus caldoxylosilyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.17 | additional information | Parageobacillus caldoxylosilyticus | enzyme is constitutively expressed | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.17 | Parageobacillus caldoxylosilyticus | - |
DSM 12041 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.17 | - |
Parageobacillus caldoxylosilyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 60 | - |
at 25°C | Parageobacillus caldoxylosilyticus |
2.7.1.17 | 185 | - |
at 65-75°C | Parageobacillus caldoxylosilyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.17 | ATP + D-xylulose | - |
Parageobacillus caldoxylosilyticus | ADP + D-xylulose 5-phosphate | - |
r | |
2.7.1.17 | additional information | enzyme is constitutively expressed | Parageobacillus caldoxylosilyticus | ? | - |
? | |
2.7.1.17 | UTP + D-xylulose | 15% of the activity with ATP | Parageobacillus caldoxylosilyticus | UDP + D-xylulose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.17 | dimer | 2 * 54000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 65 | 75 | - |
Parageobacillus caldoxylosilyticus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 30 | 77 | 30°C: about 50% of maximal activity, 77°C: about 65% of maximal activity | Parageobacillus caldoxylosilyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 65 | - |
30 min, Tris buffer, stable up to | Parageobacillus caldoxylosilyticus |
2.7.1.17 | 70 | - |
30 min, 50% loss of activity | Parageobacillus caldoxylosilyticus |
2.7.1.17 | 75 | - |
8 mM xylulose, 55% loss of activity after 30 min, 15% loss of activity after 5 min | Parageobacillus caldoxylosilyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 8 | - |
- |
Parageobacillus caldoxylosilyticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.17 | 6.7 | 9 | pH 6.7: about 50% of maximal activity, pH 9.0: about 75% of maximal activity | Parageobacillus caldoxylosilyticus |