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Literature summary extracted from

  • Touhara, K.
    Effects of mutations in pleckstrin homology domain on beta-adrenergic receptor kinase activity in intact cells (1998), Biochem. Biophys. Res. Commun., 252, 669-674.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 G protein betagamma-subunit activates, binding domain is localized to the C-terminal region of beta-ARK Bos taurus
2.7.11.15 G protein betagamma-subunit from brain, binds to the C-terminal half of the PH domain Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 wild-type beta-ARK 1 is expressed in SF9 and COS-7 cells, mutant beta-ARK 1 is cloned and expressed in COS-7 cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.15 D635K/S636K/D637K triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 D637K triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 E646K mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 K567E mutation in the PIP2-binding region of the PH domain Bos taurus
2.7.11.15 K645E mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 L647G residue of the PH domain, mutation completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit Bos taurus
2.7.11.15 additional information effects of mutations in the pleckstrin homology domain of beta-ARK on activity, Ala-insertion following Trp-643 completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit Bos taurus
2.7.11.15 Q642G mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 R578N double mutant R578N/R579N, mutation in the PIP2-binding region of the PH domain Bos taurus
2.7.11.15 R579N double mutant R578N/R579N, mutation in the PIP2-binding region of the PH domain Bos taurus
2.7.11.15 S636K triple mutant D635K/S636K/D637K, mutation in the Gbetagamma-binding region of the PH domain Bos taurus
2.7.11.15 W576A mutation in the PIP2-binding region of the PH domain Bos taurus
2.7.11.15 W643A residue of the PH domain, mutation completely abolishes beta-ARK activity and activation by the G protein betagamma-subunit Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 membrane enzyme is localized to the specific membrane compartment by betagamma subunits of G proteins and phosphatidylinositol phosphates that specifically and coordinately bind to the C- and N-terminal half, respectively, of the PH domain Bos taurus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 additional information Bos taurus plays a pivotal role in phosphorylating and desensitizing G protein-coupled receptors by vitue of pleckstrin homology domain-mediated membrane translocation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
 beta-ARK 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1 expressed in Sf9 cells Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + alpha2-adrenergic receptor agonist-induced phosphorylation Bos taurus ADP + phospho-alpha2-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor
-
 Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + muscarinic acetylcholine receptor agonist-dependent phosphorylation, subtype 2 Bos taurus ADP + phospho-muscarinic acetylcholine receptor
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 additional information mechanism and significance of the PH-domain function Bos taurus ?
-
 ?
2.7.11.15 additional information plays a pivotal role in phosphorylating and desensitizing G protein-coupled receptors by vitue of pleckstrin homology domain-mediated membrane translocation Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at, rhodopsin phosphorylation by recombinant mutant beta-ARK Bos taurus
2.7.11.15 37
-
 assay at, intact cell phosphorylation of muscarinic receptor subtype 2 and alpha2-adrenergic receptor by recombinant mutant beta-ARK Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus