EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | isoproterenol | requirement, beta-agonist, beta-adrenergic receptor as substrate | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | 2,3-diphosphoglycerate | inhibits in the millimolar range, IC50: 1.1 mM with rhodopsin as substrate; weak | Bos taurus | |
2.7.11.15 | chondroitin sulfate B | weak | Bos taurus | |
2.7.11.15 | chondroitin sulfate C | less inhibitory than heparin | Bos taurus | |
2.7.11.15 | D-glucosamine 2,6-disulfate | inhibits in the millimolar range, weak, IC50: 7.3 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | Dextran sulfate | strong, IC50: 0.00015 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | heparan sulfate | less inhibitory than heparin | Bos taurus | |
2.7.11.15 | heparin | de-N-sulfated heparin is 8fold less effective; most potent inhibitor to date; polylysine, spermine or spermidine at lower concentrations partially reverses; strong, kinetics, IC50: 0.00003 mM with beta-AR as substrate, 0.00015 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | Inositol hexaphosphate | weak, IC50: 3.6 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | Inositol hexasulfate | 270fold more potent than inositol hexaphosphate; IC50: 0.0135 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | Polyaspartic acid | IC50: 0.0013 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | Polyglutamic acid | IC50: 0.002 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | polylysine | IC50: 0.069 mM with rhodopsin as substrate; weak, more potent than spermine and spermidine | Bos taurus | |
2.7.11.15 | pyridoxal 5'-phosphate | inhibits in the millimolar range; weak, IC50: 0.9 mM with rhodopsin as substrate | Bos taurus | |
2.7.11.15 | spermidine | weak, less potent than polylysine | Bos taurus | |
2.7.11.15 | spermine | weak, less potent than polylysine | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.11.15 | 0.0053 | - |
rhodopsin | pH 7.4, 30°C, in absence of heparin | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.15 | cytosol | - |
Bos taurus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | Mg2+ | requirement | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.15 | ATP + beta-adrenergic receptor | Bos taurus | regulation of the beta-AR function in vivo | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.15 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.15 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.15 | brain | cerebral cortex | Bos taurus | - |
2.7.11.15 | cerebral cortex | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.11.15 | 4°C, several months, stable | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.15 | ATP + alpha2-adrenergic receptor | - |
Bos taurus | ADP + phospho-alpha2-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | regulation of the beta-AR function in vivo | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
2.7.11.15 | ATP + rhodopsin | light-dependent, actual substrate: light-bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.15 | ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.15 | additional information | very poor substrates: casein, phosvitin | Bos taurus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.15 | 30 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.15 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.15 | ATP | - |
Bos taurus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.11.15 | 0.00003 | - |
strong, kinetics, IC50: 0.00003 mM with beta-AR as substrate, 0.00015 mM with rhodopsin as substrate | Bos taurus | heparin | |
2.7.11.15 | 0.00015 | - |
strong, IC50: 0.00015 mM with rhodopsin as substrate | Bos taurus | Dextran sulfate | |
2.7.11.15 | 0.0013 | - |
IC50: 0.0013 mM with rhodopsin as substrate | Bos taurus | Polyaspartic acid | |
2.7.11.15 | 0.002 | - |
IC50: 0.002 mM with rhodopsin as substrate | Bos taurus | Polyglutamic acid | |
2.7.11.15 | 0.0135 | - |
IC50: 0.0135 mM with rhodopsin as substrate | Bos taurus | Inositol hexasulfate | |
2.7.11.15 | 0.069 | - |
IC50: 0.069 mM with rhodopsin as substrate | Bos taurus | polylysine | |
2.7.11.15 | 0.9 | - |
weak, IC50: 0.9 mM with rhodopsin as substrate | Bos taurus | pyridoxal 5'-phosphate | |
2.7.11.15 | 1.1 | - |
inhibits in the millimolar range, IC50: 1.1 mM with rhodopsin as substrate | Bos taurus | 2,3-diphosphoglycerate | |
2.7.11.15 | 3.6 | - |
weak, IC50: 3.6 mM with rhodopsin as substrate | Bos taurus | Inositol hexaphosphate | |
2.7.11.15 | 7.3 | - |
inhibits in the millimolar range, weak, IC50: 7.3 mM with rhodopsin as substrate | Bos taurus | D-glucosamine 2,6-disulfate |