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Literature summary extracted from

  • Woods, A.; Vertommen, D.; Neumann, D.; Tuerk, R.; Bayliss, J.; Schlattner, U.; Wallimann, T.; Carling, D.; Rider, M.H.
    Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis (2003), J. Biol. Chem., 278, 28434-28442.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.31 5'-AMP
-
 Rattus norvegicus
2.7.11.31 5-amino-4-imidazolecarboxamide ribonucleoside
-
 Rattus norvegicus
2.7.11.31 adiponectin
-
 Rattus norvegicus
2.7.11.31 leptin
-
 Rattus norvegicus
2.7.11.31 metformin antidiabetic drug Rattus norvegicus
2.7.11.31 additional information AMPK can also be activated by hyperosmotic stress Rattus norvegicus
2.7.11.31 rosiglitazone antidiabetic drug Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.31 transfection of CCL13 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.31 S485A site-directed mutagenesis, non-phosphorylatable mutant Rattus norvegicus
2.7.11.31 S485D site-directed mutagenesis Rattus norvegicus
2.7.11.31 T172E site-directed mutagenesis Rattus norvegicus
2.7.11.31 T258A site-directed mutagenesis, non-phosphorylatable mutant Rattus norvegicus
2.7.11.31 T258D site-directed mutagenesis Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.31 40000
-
 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus
2.7.11.31 63000
-
 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus activated AMPK acts to down-regulate ATP-consuming pathways such as fatty acid synthesis by phosphorylating and inactivating acetyl-CoA carboxylase and protein synthesis by promoting the phosphorylation of eukaryotic elongation factor-2, in heart AMPK activation stimulates glycolysis by increasing glucose uptake ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.31 wild-type and mutant enzymes, expressed in bacteria Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + histone 2A
-
 Rattus norvegicus ?
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] activated AMPK acts to down-regulate ATP-consuming pathways such as fatty acid synthesis by phosphorylating and inactivating acetyl-CoA carboxylase and protein synthesis by promoting the phosphorylation of eukaryotic elongation factor-2, in heart AMPK activation stimulates glycolysis by increasing glucose uptake Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.31 heterotrimer 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus
2.7.11.31 More heterotrimeric serine/threonine kinase consists of a catalytic alpha-subunit and 2 regulatory subunits beta and gamma, each subunit exists as multiple isoforms, alpha1, alpha2, beta1, beta2, gamma1, gamma2 and gamma3, giving 12 different possible combinantions of holoenzyme with different tissue distribution and subcellular localization Rattus norvegicus