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Literature summary extracted from

  • Eis, C.; Nidetzky, B.
    Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors (2002), Biochem. J., 363, 335-340.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 2-deoxy-2-fluoro-alpha-D-glucose 1-fluoride inhibits reaction with alpha,alpha-trehalose Schizophyllum commune
2.4.1.231 2-deoxy-2-fluoro-glucose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 alpha-D-galactose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 alpha-D-glucose
-
 Schizophyllum commune
2.4.1.231 alpha-D-glucose 1-fluoride competitive with respect to alpha,alpha-trehalose in the phosphorolysis direction Schizophyllum commune
2.4.1.231 alpha-D-glucose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 alpha-D-glucuronic acid 1-phosphate
-
 Schizophyllum commune
2.4.1.231 alpha-D-mannose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 alpha-D-xylose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 D-galactose
-
 Schizophyllum commune
2.4.1.231 D-xylose
-
 Schizophyllum commune
2.4.1.231 vanadate phosphate mimic, inhibits phosphorolysis of alpha-D-glucose 1-fluoride by forming a ternary complex Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 5.9
-
 alpha-D-glucose 1-fluoride 30°C, pH 6.6 Schizophyllum commune

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate Schizophyllum commune
-
 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Schizophyllum commune
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.231 alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate mechanism Schizophyllum commune
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Schizophyllum commune alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.231 alpha-D-glucose 1-fluoride + phosphate
-
 Schizophyllum commune alpha-D-glucose 1-phosphate + fluoride
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.231 0.11
-
 alpha-D-glucose 1-fluoride 30°C, pH 6.6 Schizophyllum commune

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 0.3
-
 alpha-D-glucose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 1.6
-
 alpha-D-mannose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 2.4
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 10.6
-
 2-deoxy-2-fluoro-glucose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 11
-
 alpha-D-xylose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 11.5
-
 alpha-D-galactose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 13.4
-
 alpha-D-glucuronic acid 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 1000
-
 D-galactose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 1000
-
 D-xylose 30°C, pH 6.6 Schizophyllum commune