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Literature summary extracted from

  • Eis, C.; Nidetzky, B.
    Characterization of trehalose phosphorylase from Schizophyllum commune (1999), Biochem. J., 341, 385-393.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 NaCl competitive with respect to phosphate Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 2.4
-
 phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 2.8
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 39
-
 alpha-D-glucose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 91
-
 alpha,alpha-trehalose 30°C, pH 6.6 Schizophyllum commune

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.231 60000
-
 gel filtration Schizophyllum commune
2.4.1.231 61000
-
 SDS-PAGE Schizophyllum commune

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + phosphate Schizophyllum commune
-
 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Schizophyllum commune
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.231
-
 Schizophyllum commune

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.231 alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate mechanism Schizophyllum commune
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.231 9.85
-
-
 Schizophyllum commune

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha,alpha-trehalose + arsenate
-
 Schizophyllum commune alpha-D-glucose 1-arsenate + alpha-D-glucose
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Schizophyllum commune alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.231 monomer 1 * 60000, SDS-PAGE, gel filtration Schizophyllum commune

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.231 4
-
 unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize Schizophyllum commune
2.4.1.231 20
-
 unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize Schizophyllum commune

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.231 10
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 10
-
 alpha-D-glucose 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 14
-
 phosphate 30°C, pH 6.6 Schizophyllum commune
2.4.1.231 15
-
 alpha,alpha-trehalose 30°C, pH 6.6 Schizophyllum commune

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.231 6.2
-
 reverse reaction Schizophyllum commune
2.4.1.231 6.6
-
 forward reaction Schizophyllum commune

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.231 6.2 6.6 decrease in enzyme activity below pH 6.2 and above pH 6.6 Schizophyllum commune

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 67
-
 NaCl 30°C, pH 6.6 Schizophyllum commune

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.231 Schizophyllum commune isoelectric focusing between pH 4 and pH 6.5
-
 4.8