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Literature summary extracted from
- Purification and properties of a UDP glucose:thiohydroximate glucosyltransferase from higher plants (1971), Phytochemistry, 10, 2279-2288.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.195 | 1,10-phenanthroline | 0.1 mM, activation | Tropaeolum majus |  |
| 2.4.1.195 | 2,2'-dipyridyl | 1.0 mM, activation | Tropaeolum majus | |
| 2.4.1.195 | 2-mercaptoethanol | requirement | Tropaeolum majus | |
| 2.4.1.195 | 2-mercaptoethanol | activity dissappears in absence | Tropaeolum majus | |
| 2.4.1.195 | Cysteine hydrochloride | 1.0 mM, activation | Tropaeolum majus | |
| 2.4.1.195 | dithiothreitol | 1.0 mM, activation | Tropaeolum majus | |
| 2.4.1.195 | EDTA | 0.01 mM, activation | Tropaeolum majus | |
| 2.4.1.195 | glutathione | 1.0 mM, activation | Tropaeolum majus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.195 | HgCl2 | - |
Tropaeolum majus | |
| 2.4.1.195 | additional information | no inhibition by KCN or iodoacetic acid, only moderately inhibited by N-ethylmaleimide | Tropaeolum majus | |
| 2.4.1.195 | p-chloromercuribenzoate | - |
Tropaeolum majus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.195 | 1.05 | - |
4-methylthiobutyrothiohydroximate | pH 7.4, 30°C | Tropaeolum majus | |
| 2.4.1.195 | 1.47 | - |
UDPglucose | pH 7.4, 30°C, 4-methylthiobutyrothiohydroximate | Tropaeolum majus | |
| 2.4.1.195 | 1.54 | - |
UDPglucose | pH 7.4, 30°C, phenylacetothiohydroximate | Tropaeolum majus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.195 | 50000 | - |
gel filtration | Tropaeolum majus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | Sinapis alba | - |
UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | Nasturtium officinale | - |
UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | Armoracia rusticana | - |
UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | Tropaeolum majus | integral step in the biosynthesis of benzylglucosinolate | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | Nasturtium officinale R.Br. | - |
UDP + desulfoglucotropeolin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.195 | Armoracia rusticana | - |
- |
- |
| 2.4.1.195 | Nasturtium officinale | - |
R.Br. | - |
| 2.4.1.195 | Nasturtium officinale R.Br. | - |
R.Br. | - |
| 2.4.1.195 | Sinapis alba | - |
- |
- |
| 2.4.1.195 | Tropaeolum majus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.195 | - |
Tropaeolum majus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.195 | leaf | - |
Sinapis alba | - |
| 2.4.1.195 | leaf | - |
Tropaeolum majus | - |
| 2.4.1.195 | leaf | - |
Nasturtium officinale | - |
| 2.4.1.195 | leaf | - |
Armoracia rusticana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.195 | 45 | - |
- |
Tropaeolum majus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.195 | -15°C, freeze-dried protein, specific activity remains unchanged for more than 3 months | Tropaeolum majus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.195 | additional information | acetothiohydroximate, phenylacetohydroximic acid, ethanol, 2-mercaptoethanol and cysteine hydrochloride are no substrates | Tropaeolum majus | ? | - |
? | |
| 2.4.1.195 | UDP-glucose + 4-methylthiobutyrothiohydroximate | relative reaction rate 77% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-4-methylthiobutyrothiohydroximate | - |
? | |
| 2.4.1.195 | UDP-glucose + benzothiohydroximate | relative reaction rate 10% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-benzothiohydroximate | - |
? | |
| 2.4.1.195 | UDP-glucose + isobutyrothiohydroximate | relative reaction rate 41% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-isobutyrothiohydroximate | - |
? | |
| 2.4.1.195 | UDPglucose + butyrothiohydroximate | relative reaction rate 70% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-butyrothiohydroximate | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | - |
Sinapis alba | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | - |
Tropaeolum majus | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | - |
Nasturtium officinale | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | - |
Armoracia rusticana | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | integral step in the biosynthesis of benzylglucosinolate | Tropaeolum majus | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + phenylacetothiohydroximate | - |
Nasturtium officinale R.Br. | UDP + desulfoglucotropeolin | - |
? | |
| 2.4.1.195 | UDPglucose + propiothiohydroximate | relative reaction rate 50% | Tropaeolum majus | UDP + S-(alpha-D-glucopyranosyl)-propiothiohydroximate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.195 | 6.5 | 7.5 | greater activity in phosphate and Tris-HCl buffers than in Tris-maleate | Tropaeolum majus |
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