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Literature summary extracted from

  • Sekura, R.D.; Duffel, M.W.; Jakoby, W.B.
    Aryl sulfotransferases (1981), Methods Enzymol., 77, 197-206.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.2.1 transferase II is unstable to isoelectric focusing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 2,6-Dichloro-4-nitrophenol
-
 Rattus norvegicus
2.8.2.1 Pentachlorophenol
-
 Rattus norvegicus
2.8.2.9 2,6-Dichloro-4-nitrophenol
-
 Rattus norvegicus
2.8.2.9 adenosine 3',5'-bisphosphate
-
 Rattus norvegicus
2.8.2.9 Aryl sulfates
-
 Rattus norvegicus
2.8.2.9 Pentachlorophenol
-
 Rattus norvegicus
2.8.2.9 thiol reagents
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.9 0.024
-
 3'-phosphoadenylylsulfate pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.1
-
 2-naphthol pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.34
-
 4-Chlorophenol
-
 Rattus norvegicus
2.8.2.9 0.43
-
 epinephrine pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.46
-
 tyramine pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.91
-
 tyrosine methyl ester
-
 Rattus norvegicus
2.8.2.9 0.92
-
 phenol pH 5.5, 37°C Rattus norvegicus
2.8.2.9 1.4
-
 4-methoxyphenol pH 5.5, 37°C Rattus norvegicus
2.8.2.9 6.9
-
 2-cyanoethyl-N-hydroxythioacetamide pH 5.5, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.1 KCl 50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B, aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M KCl or NaCl Rattus norvegicus
2.8.2.1 additional information
-
 Rattus norvegicus
2.8.2.1 NaCl aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M NaCl or KCl Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 33500
-
 2 * 33500, arylsulfotransferase III, SDS-PAGE Rattus norvegicus
2.8.2.1 61000
-
 arylsulfotransferase III, gel filtration Rattus norvegicus
2.8.2.1 64000
-
 arylsulfotransferase I or II, gel filtration Rattus norvegicus
2.8.2.9 33500
-
 2 * 33500, SDS-PAGE Rattus norvegicus
2.8.2.9 61000
-
 gel filtration Rattus norvegicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.8.2.9 Acetone 5% acetone is not inhibitory Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Rattus norvegicus
-
-
-
2.8.2.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 arylsulfotransferase I, II, III Rattus norvegicus
2.8.2.9 aryl sulfotransferase IV Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 liver
-
 Rattus norvegicus
-
2.8.2.9 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 0.25
-
 arylsulfotransferase I Rattus norvegicus
2.8.2.1 0.282
-
 arylsulfotransferase II Rattus norvegicus
2.8.2.9 0.54
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 -80°C, transferase III may deteriorate at any stage of purification Rattus norvegicus
2.8.2.1 4°C, sodium phosphate, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, protein concentration 0.5-1 mg/ml, transferase I, II lose about 5% of their activity per week Rattus norvegicus
2.8.2.9 4°C, sodium phosphate buffer, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, 5% loss of activity per week at protein concentration between 0.5 and 1 mg/ml Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + 4-methoxyphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylylsulfate + phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
 ?
2.8.2.1 additional information activity with various substrates at different pH values Rattus norvegicus ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetamide
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetimidate
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 2-naphthol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 4-chlorophenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + 4-methoxyphenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + epinephrine
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + L-tyrosine methyl ester
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + phenol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
 ?
2.8.2.9 3'-phosphoadenylylsulfate + tyramine
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.1 dimer 2 * 64000, arylsulfotransferase I or II Rattus norvegicus
2.8.2.1 dimer 2 * 33500, arylsulfotransferase III, SDS-PAGE Rattus norvegicus
2.8.2.9 dimer 2 * 33500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.1 additional information
-
 additional information
-
 Rattus norvegicus
2.8.2.1 0.113
-
 phenol isoenzyme I Rattus norvegicus
2.8.2.1 0.122
-
 phenol isoenzyme II Rattus norvegicus
2.8.2.1 0.667
-
 4-Chlorophenol isoenzyme II Rattus norvegicus
2.8.2.1 0.783
-
 2-naphthol isoenzyme I Rattus norvegicus
2.8.2.1 0.8
-
 4-Chlorophenol isoenzyme I Rattus norvegicus
2.8.2.1 0.9
-
 2-naphthol isoenzyme II Rattus norvegicus
2.8.2.1 1.55
-
 4-methoxyphenol isoenzyme I Rattus norvegicus
2.8.2.1 2
-
 4-methoxyphenol isoenzyme II Rattus norvegicus
2.8.2.9 0.03
-
 phenol
-
 Rattus norvegicus
2.8.2.9 0.0467
-
 tyramine pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.122
-
 epinephrine pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.233
-
 tyrosine methyl ester pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.233
-
 2-cyanoethyl-N-hydroxythioacetimidate pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.3
-
 4-Chlorophenol
-
 Rattus norvegicus
2.8.2.9 0.45
-
 4-methoxyphenol pH 5.5, 37°C Rattus norvegicus
2.8.2.9 0.7
-
 2-naphthol pH 5.5, 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 5.5
-
 with substrate 2-naphthol, aryl sulfotransferase I and II Rattus norvegicus
2.8.2.1 6.5
-
 with substrate 2-naphthol, aryl sulfotransferase II Rattus norvegicus
2.8.2.9 5.5
-
 2-naphthol, no formation of sulfate esters from tyrosine methyl ester, epinephrine, octopamine, tyramine, serotonin, N-acetylserotonin Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.9 Rattus norvegicus
-
-
 5.8