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Literature summary extracted from

  • Sanders, J.P.; Van der Geyten, S.; Kaptein, E.; Darras, V.M.; Kuhn, E.R.; Leonard, J.L.; Visser, T.J.
    Cloning and characterization of type III iodothyronine deiodinase from the fish Oreochromis niloticus (1999), Endocrinology, 140, 3666-3673.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.99.3 DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells Oreochromis niloticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.3 aurothioglucose recombinant and native isozyme type III Oreochromis niloticus
1.21.99.3 iodoacetate recombinant and native isozyme type III Oreochromis niloticus
1.21.99.3 propylthiouracil slight inhibition of recombinant and native isozyme type III Oreochromis niloticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.3 0.00002
-
 3,3',5-triiodo-L-thyronine pH 7.2, 37°C Oreochromis niloticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.99.3 microsome isozyme type III Oreochromis niloticus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.3 Oreochromis niloticus Q9I966 isozyme type III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.99.3 recombinant from COS-1 cells Oreochromis niloticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.99.3 brain isozyme type III Oreochromis niloticus
-
1.21.99.3 gill gene expression Oreochromis niloticus
-
1.21.99.3 gut very low activity Oreochromis niloticus
-
1.21.99.3 gut isozyme type III Oreochromis niloticus
-
1.21.99.3 kidney low activity Oreochromis niloticus
-
1.21.99.3 kidney isozyme type III Oreochromis niloticus
-
1.21.99.3 liver low activity Oreochromis niloticus
-
1.21.99.3 liver isozyme type III Oreochromis niloticus
-
1.21.99.3 muscle very low activity Oreochromis niloticus
-
1.21.99.3 muscle isozyme type III Oreochromis niloticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.3 3,3',5-triiodo-L-thyronine + AH2 best substrate Oreochromis niloticus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
 ?
1.21.99.3 3,3',5-triiodo-L-thyronine + AH2 isozyme type III Oreochromis niloticus 3,3'-diiodo-L-thyronine + iodide + A + H+
-
 ?
1.21.99.3 3,3',5-triiodo-L-thyronine sulfate + AH2 isozyme type III Oreochromis niloticus 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
-
 ?
1.21.99.3 3,3',5-triiodo-L-thyronine sulfate + AH2 about 14% of the activity with 3,3',5-triiodo-L-thyronine Oreochromis niloticus 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
-
 ?
1.21.99.3 L-thyroxine + AH2 very low activity Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
1.21.99.3 L-thyroxine + AH2 isozyme type III Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?
1.21.99.3 L-thyroxine + AH2 5-deiodinase activity Oreochromis niloticus 3,3',5'-triiodo-L-thyronine + iodide + A + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.99.3 37
-
 assay at Oreochromis niloticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.99.3 7.2
-
 assay at Oreochromis niloticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.99.3 dithiothreitol i.e. DTT Oreochromis niloticus