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Literature summary extracted from

  • Ichiki, H.; Tanaka, Y.; Mochizuki, K.; Yoshimatsu, K.; Sakurai, T.; Fujiwara, T.
    Purification, characterization, and genetic analysis of Cu-containing dissimilatory nitrite reductase from a denitrifying halophilic archaeon, Haloarcula marismortui (2001), J. Bacteriol., 183, 4149-4156.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.1 diethyldithiocarbamate 1 mM, complete inhibition Haloarcula marismortui
1.7.2.1 EDTA 1 mM, 70% inhibition after 1 h Haloarcula marismortui
1.7.2.1 KCN 1 mM, complete inhibition Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper blue copper enzyme, 1 type 1 and 1 type 2 Cu atoms per subunit Haloarcula marismortui
1.7.2.1 copper green copper enzyme Haloferax denitrificans
1.7.2.1 copper green copper enzyme Achromobacter cycloclastes
1.7.2.1 copper blue copper enzyme Fusarium oxysporum
1.7.2.1 copper blue copper enzyme Achromobacter xylosoxidans
1.7.2.1 KCl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
1.7.2.1 LiCl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
1.7.2.1 NaNO3 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui
1.7.2.1 NH4Cl 2 M, enzyme is activated by high salt concentrations Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 36500
-
 3 * 36500, calculated from sequence, crystal structure Achromobacter xylosoxidans
1.7.2.1 37000
-
 3 * 37000, calculated from sequence, crystal structure Achromobacter cycloclastes
1.7.2.1 41800
-
 2 * 41800, SDS-PAGE Fusarium oxysporum
1.7.2.1 46000
-
 3 * 46000, SDS-PAGE Haloarcula marismortui
1.7.2.1 64000
-
 2 * 64000, SDS-PAGE Haloferax denitrificans
1.7.2.1 69000
-
 gel filtration Achromobacter cycloclastes
1.7.2.1 70000
-
 gel filtration Achromobacter xylosoxidans
1.7.2.1 83300
-
 gel filtration Fusarium oxysporum
1.7.2.1 103000
-
 sedimentation equilibrium Achromobacter xylosoxidans
1.7.2.1 105000
-
 sedimentation equlibrium Achromobacter cycloclastes
1.7.2.1 127000
-
 gel filtration Haloferax denitrificans
1.7.2.1 167000
-
 gel filtration Haloarcula marismortui

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Achromobacter cycloclastes P25006
-
-
1.7.2.1 Achromobacter xylosoxidans
-
-
-
1.7.2.1 Fusarium oxysporum
-
-
-
1.7.2.1 Haloarcula marismortui
-
-
-
1.7.2.1 Haloferax denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 butyl-toyopearl, Sepharose CL-6B, Sephacryl S-300, octyl-sepharose, hydroxyapatite Haloarcula marismortui

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 960
-
 in the presence of 2 M NaCl Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + ferrocytochrome c
-
 Haloarcula marismortui nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c
-
 Fusarium oxysporum nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c
-
 Achromobacter xylosoxidans nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c
-
 Haloferax denitrificans nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c
-
 Achromobacter cycloclastes nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 NO2- + Na2S2O4 pysiological electron donor is unknown, no activity with methyl viologen, phenazine methosulfate or N,N,N',N',-tetramethyl-p-phenylenediamine Haloarcula marismortui NO + Na2S2O3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 dimer 2 * 64000, SDS-PAGE Haloferax denitrificans
1.7.2.1 dimer 2 * 41800, SDS-PAGE Fusarium oxysporum
1.7.2.1 trimer 3 * 46000, SDS-PAGE Haloarcula marismortui
1.7.2.1 trimer 3 * 37000, calculated from sequence, crystal structure Achromobacter cycloclastes
1.7.2.1 trimer 3 * 36500, calculated from sequence, crystal structure Achromobacter xylosoxidans