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Literature summary extracted from

  • Prudencio, M.; Eady, R.R.; Sawers, G.
    The blue copper-containing nitrite reductase from Alcaligenes xylosoxidans: cloning of the nirA gene and characterization of the recombinant enzyme (1999), J. Bacteriol., 181, 2323-2329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.1 expression in Escherichia coli Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.035
-
 NO2-
-
 Achromobacter xylosoxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper blue copper enzyme that shows little absorbance in the 460 nm range, purified recombinant nonactivated enzyme contains 1.97 mol Copper/mol enzyme, the CuSO4 activated enzyme contains 5.97 mol copper/mol enzyme i.e. 6 copper atoms per trimer, CuSO4 activation restores type 2 copper centers which are the sites of catalysis Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 recombinant enzyme Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 10.8
-
 recombinant enzyme, without activation, enzyme has only type 1 copper centers Achromobacter xylosoxidans
1.7.2.1 167.7
-
 recombinant enzyme, after activation with CuSO4 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + ferrocytochrome c
-
 Achromobacter xylosoxidans nitric oxide + H2O + ferricytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 trimer recombinant enzyme Achromobacter xylosoxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.1 80
-
 native and purified enzyme retain 50% activity after 20 min exposure Achromobacter xylosoxidans