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Literature summary extracted from

  • Siddiqui, M.A.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    In vitro heat effect on heterooligomeric subunit assembly of thermostable indolepyruvate ferredoxin oxidoreductase (1998), FEBS Lett., 434, 372-376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.8 gene cloning and sequence analysis of the IOR gene, 2 genes iorA and iorB, encoding alpha and beta subunits are tandemly arranged, KOD1-IOR overproduced in anaerobically incubated Escherichia coli BL21(DE3) Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.8 24000
-
 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 71000
-
 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 192000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Pyrococcus furiosus
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Thermococcus kodakarensis
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.8 Pyrococcus furiosus
-
-
-
1.2.7.8 Thermococcus kodakarensis
-
 KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.8
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus kodakarensis S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 indolepyruvate + CoA + oxidized methylviologen
-
 Pyrococcus furiosus indoleacetyl-CoA + CO2 + reduced methylviologen
-
 ?
1.2.7.8 indolepyruvate + CoA + oxidized methylviologen
-
 Thermococcus kodakarensis indoleacetyl-CoA + CO2 + reduced methylviologen
-
 ?
1.2.7.8 p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus kodakarensis p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus kodakarensis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.7.8 tetramer
-
 Thermococcus kodakarensis
1.2.7.8 tetramer 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.8 70
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.8 45 80
-
 Thermococcus kodakarensis