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Literature summary extracted from

  • Mai, X.; Adams, M.W.
    Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A new enzyme involved in peptide fermentation (1994), J. Biol. Chem., 269, 16726-16732.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.8 thiamine diphosphate activity increases about 8fold, Km for thiamine diphosphate 0.004 mM Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.8 Cu2+
-
 Pyrococcus furiosus
1.2.7.8 cyanide
-
 Pyrococcus furiosus
1.2.7.8 KCN
-
 Pyrococcus furiosus
1.2.7.8 additional information not inhibited by CO, unaffected by sodium nitrite, not inhibited by sodium fluoride or sodium azide, sodium benzoate, phenylacetate, pyruvate, 2-ketoglutarate and oxamate have no effect on activity Pyrococcus furiosus
1.2.7.8 Zn2+
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.8 0.017
-
 CoA pH 8.4, 80°C, substrate indolepyruvate Pyrococcus furiosus
1.2.7.8 0.021
-
 CoA pH 8.4, 80°C, substrate phenylpyruvate Pyrococcus furiosus
1.2.7.8 0.048
-
 oxidized ferredoxin pH 8.4, 80°C Pyrococcus furiosus
1.2.7.8 0.095
-
 phenylpyruvate pH 8.4, 80°C Pyrococcus furiosus
1.2.7.8 0.11
-
 p-hydroxyphenylpyruvate pH 8.4, 80°C Pyrococcus furiosus
1.2.7.8 0.25
-
 Indolepyruvate pH 8.4, 80°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.8 cytoplasm
-
 Pyrococcus furiosus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.8 23000
-
 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 66000
-
 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
1.2.7.8 180000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Pyrococcus furiosus involved in peptide fermentation and metabolism of aromatic amino acids S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.8 Pyrococcus furiosus
-
 DSM 3638
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.8 enzyme is sensitive to inactivation by O2, losing 50% of its activity after exposure to air for 20 min at 23°C Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.8
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.8 38
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin involved in peptide fermentation and metabolism of aromatic amino acids Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+
-
 r
1.2.7.8 2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA
-
 r
1.2.7.8 2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus CO2 + reduced ferredoxin + isovaleryl-CoA
-
 r
1.2.7.8 additional information pyruvate and transaminated forms of the amino acids aspartate, valine, glutamate and alanine, 2-ketobutyrate, oxalacetate, 2-ketoisovalerate, 2-ketoglutarate, 2-ketomalonate and phenylglyoxylate are not oxidized Pyrococcus furiosus ?
-
 ?
1.2.7.8 p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.7.8 tetramer 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.8 90
-
 exhibits optimal activity above at pH 8.0 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.8 70 90 shows dramatic increase in activity above 70°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.8 80
-
 virtually inactive at 25°C, quite thermostable, with a half-life of activity at 80°C of about 80 min under anaerobic conditions Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.8 8.5 10.5 at 80°C Pyrococcus furiosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.7.8 8 10 not active at pH 5.2 and pH 11.0 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.8 7.5
-
 KCN pH 8.4, 80°C Pyrococcus furiosus