BRENDA - Enzyme Database show

Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism

Mukund, S.; Adams, M.W.; J. Biol. Chem. 268, 13592-13600 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.B2
arsenite
5 mM, 50% inhibition after min
Thermococcus litoralis
1.2.7.B2
cyanide
5 mM, 50% inhibition after min
Thermococcus litoralis
1.2.7.B2
iodoacetate
5 mM, 50% inhibition after min
Thermococcus litoralis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Iron
the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states
Thermococcus litoralis
1.2.7.B2
Tungsten
tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme
Thermococcus litoralis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.B2
30000
-
gel filtration
Thermococcus litoralis
1.2.7.B2
70000
-
4 * 70000, SDS-PAGE
Thermococcus litoralis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.B2
Thermococcus litoralis
Q56303
-
-
1.2.7.B2
Thermococcus litoralis DSM 5473
Q56303
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.7.B2
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h
Thermococcus litoralis
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.B2
-
Thermococcus litoralis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.B2
6.8
-
pH 8.4, 80°C
Thermococcus litoralis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.B2
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis
acetate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis DSM 5473
acetate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
butyrate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
butyrate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis DSM 5473
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
formate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
formate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis
?
-
-
-
-
1.2.7.B2
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis DSM 5473
?
-
-
-
-
1.2.7.B2
propionaldehyde + H2O + oxidized methyl viologen
77% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
propionate + H+ + reduced methyl viologen
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.B2
tetramer
4 * 70000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
assay at
Thermococcus litoralis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
2 h, 50% loss of activity
Thermococcus litoralis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.B2
8.4
-
assay at
Thermococcus litoralis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.7.B2
arsenite
5 mM, 50% inhibition after min
Thermococcus litoralis
1.2.7.B2
cyanide
5 mM, 50% inhibition after min
Thermococcus litoralis
1.2.7.B2
iodoacetate
5 mM, 50% inhibition after min
Thermococcus litoralis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Iron
the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states
Thermococcus litoralis
1.2.7.B2
Tungsten
tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.B2
30000
-
gel filtration
Thermococcus litoralis
1.2.7.B2
70000
-
4 * 70000, SDS-PAGE
Thermococcus litoralis
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.7.B2
the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h
Thermococcus litoralis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.B2
-
Thermococcus litoralis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.B2
6.8
-
pH 8.4, 80°C
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.B2
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis
acetate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
acetaldehyde + H2O + oxidized methyl viologen
-
644682
Thermococcus litoralis DSM 5473
acetate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
butyrate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
butyraldehyde + H2O + oxidized methyl viologen
10% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
butyrate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin
-
644682
Thermococcus litoralis DSM 5473
formate + 2 H+ + 2 reduced ferredoxin
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
formate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
formaldehyde + H2O + oxidized methyl viologen
63% compared to the activity with acetyladehyde
644682
Thermococcus litoralis DSM 5473
formate + H+ + reduced methyl viologen
-
-
-
?
1.2.7.B2
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis
?
-
-
-
-
1.2.7.B2
additional information
activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde
644682
Thermococcus litoralis DSM 5473
?
-
-
-
-
1.2.7.B2
propionaldehyde + H2O + oxidized methyl viologen
77% compared to the activity with acetyladehyde
644682
Thermococcus litoralis
propionate + H+ + reduced methyl viologen
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.B2
tetramer
4 * 70000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
assay at
Thermococcus litoralis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.7.B2
80
-
2 h, 50% loss of activity
Thermococcus litoralis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.B2
8.4
-
assay at
Thermococcus litoralis