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Literature summary extracted from
- Phenylacetyl-CoA:acceptor oxidoreductase, a new a-oxidizing enzyme that produces phenylglyoxylate. Assay, membrane localization, and differential production in Thauera aromatica (1998), Arch. Microbiol., 169, 509-516.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.5.1 | additional information | enzyme activity is induced under denitrifying conditions with phenylalanine and phenylacetate | Thauera aromatica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.5.1 | CN- | irreversible inactivation at low concentrations, faster inactivation at more alkaline pH | Thauera aromatica |  |
| 1.17.5.1 | additional information | low enzyme activity in cells grown with phenylglyoxylate, no activity in cells grown with benzoate and nitrate or after aerobic growth with phenylacetate, not inhibited by sodium salts of azide, thiocyanate, nitrate, nitrite, fluoride, cyanate, bipyridine, hydroxylamine, hydroxyurea, each at 10 mM | Thauera aromatica |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.5.1 | additional information | - |
additional information | - |
Thauera aromatica |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.17.5.1 | membrane | bound | Thauera aromatica | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.5.1 | additional information | Thauera aromatica | anaerobic metabolism of phenylacetate, catalyzes the first step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the second step being carried out by EC 3.1.2.25, cytochrome c probably does not act as physiological electron acceptor | ? | - |
? | |
| 3.1.2.25 | additional information | Thauera aromatica | catalyzes the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1 | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.5.1 | Thauera aromatica | - |
- |
- |
| 1.17.5.1 | Thauera aromatica | - |
strain DSM 6984 | - |
| 3.1.2.25 | Thauera aromatica | - |
- |
- |
| 3.1.2.25 | Thauera aromatica | - |
strain DSM 6984 | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.17.5.1 | oxygen-insensitive, 73% of the initial activity is recovered after 3 h incubation in air on ice | Thauera aromatica |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.17.5.1 | phenylacetyl-CoA + H2O + 2 quinone = phenylglyoxylyl-CoA + 2 quinol | the enzyme from Thauera aromatica is a membrane-bound molybdenum-iron-sulfur protein, the enzyme is specific for phenylacetyl-CoA as substrate, phenylacetate, acetyl-CoA, benzoyl-CoA, propionyl-CoA, crotonyl-CoA, succinyl-CoA and 3-hydroxybenzoyl-CoA cannot act as substrates, the oxygen atom introduced into the product, phenylglyoxylyl-CoA, is derived from water and not molecular oxygen, duroquinone, menaquinone and 2,6-dichlorophenolindophenol(DCPIP) can act as acceptor, but the likely physiological acceptor is ubiquinone, a second enzyme, EC 3.1.2.25, phenylglyoxylyl-CoA hydrolase, converts the phenylglyoxylyl-CoA formed into phenylglyoxylate | Thauera aromatica | |
| 3.1.2.25 | phenylglyoxylyl-CoA + H2O = phenylglyoxylate + CoA | This is the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1, phenylacetyl-CoA dehydrogenase | Thauera aromatica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.5.1 | 0.03 | - |
pH 7.5, 30°C | Thauera aromatica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.5.1 | additional information | no substrate: phenylacetate, inactive electron acceptors: NAD+, NADP+, FAD, FMN, triphenyltetrazoliumchloride | Thauera aromatica | ? | - |
? | |
| 1.17.5.1 | additional information | anaerobic metabolism of phenylacetate, catalyzes the first step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the second step being carried out by EC 3.1.2.25, cytochrome c probably does not act as physiological electron acceptor | Thauera aromatica | ? | - |
? | |
| 1.17.5.1 | phenylacetyl-CoA + H2O + 2,6-dichlorophenolindophenol | four-electron oxidation, uses dichlorophenolindophenol as artificial electron acceptor, 2 mol dichlorophenolindophenol is reduced per mol phenylacetyl-CoA, catalyzes the anaerobic oxidation of the methylene group in the alpha-position to the CoA-activated carboxyl group, molecular oxygen is not required for the oxidation | Thauera aromatica | phenylglyoxylyl-CoA + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.17.5.1 | phenylacetyl-CoA + H2O + quinone | - |
Thauera aromatica | phenylglyoxylyl-CoA + quinol | - |
? | |
| 3.1.2.25 | additional information | catalyzes the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1 | Thauera aromatica | ? | - |
? | |
| 3.1.2.25 | phenylglyoxylyl-CoA + H2O | - |
Thauera aromatica | phenylglyoxylate + CoA | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.5.1 | More | catalyzes the first step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the second step being carried out by EC 3.1.2.25 | Thauera aromatica |
| 1.17.5.1 | oxidase, phenylacetyl coenzyme A (alpha-oxidizing) | - |
Thauera aromatica |
| 1.17.5.1 | phenylacetyl-CoA alpha-carbon-oxidizing oxidase | - |
Thauera aromatica |
| 1.17.5.1 | phenylacetyl-CoA-acceptor oxidoreductase | - |
Thauera aromatica |
| 1.17.5.1 | phenylacetyl-CoA:acceptor oxidoreductase | - |
Thauera aromatica |
| 3.1.2.25 | More | an enzyme preparation of phenylacetyl-CoA:acceptor oxidoreductase, EC 1.17.5.1, catalyzes the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1 | Thauera aromatica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.5.1 | 30 | - |
assay at | Thauera aromatica |
| 3.1.2.25 | 30 | - |
assay at | Thauera aromatica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.5.1 | 7 | - |
50 mM potassium phosphate buffer | Thauera aromatica |
| 3.1.2.25 | 7.5 | - |
assay at | Thauera aromatica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.5.1 | 6.5 | 7.5 | with 50 mM potassium phosphate buffer: 60% of optimum activity at pH 6.5, 87% of optimum activity at pH 7.5, with 50 mM Tris-HCl buffer: 60% of optimum activity at pH 7.5 | Thauera aromatica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.5.1 | additional information | no requirement of molecular oxygen for phenylacetyl-CoA oxidation | Thauera aromatica |
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