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Literature summary extracted from
- Purification and properties of xanthine oxidase from Enterobacter cloacae (1981), Agric. Biol. Chem., 45, 425-432.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.3.2 | Ag+ | - |
Enterobacter cloacae |  |
| 1.17.3.2 | ascorbic acid | - |
Enterobacter cloacae | |
| 1.17.3.2 | Co2+ | - |
Enterobacter cloacae | |
| 1.17.3.2 | Cu2+ | - |
Enterobacter cloacae | |
| 1.17.3.2 | Fe3+ | - |
Enterobacter cloacae | |
| 1.17.3.2 | H2O2 | - |
Enterobacter cloacae | |
| 1.17.3.2 | p-chloromercuribenzoate | - |
Enterobacter cloacae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.3.2 | Iron | iron-molybdenum protein | Enterobacter cloacae | |
| 1.17.3.2 | Molybdenum | an iron-molybdenum protein | Enterobacter cloacae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 69000 | - |
2 * 69000, SDS-PAGE | Enterobacter cloacae |
| 1.17.3.2 | 128000 | - |
gel filtration | Enterobacter cloacae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.3.2 | Enterobacter cloacae | - |
KY 3074 | - |
| 1.17.3.2 | Enterobacter cloacae KY 3074 | - |
KY 3074 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.17.3.2 | additional information | - |
Enterobacter cloacae |
| 1.17.3.2 | proteolytic modification | - |
Enterobacter cloacae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.3.2 | - |
Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 15.2 | - |
- |
Enterobacter cloacae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.3.2 | 6-mercaptopurine + H2O + O2 | 4.4% of activity with xanthin | Enterobacter cloacae | 6-mercapto-7,9-dihydropurin-8-one + H2O2 | - |
? | |
| 1.17.3.2 | adenine + H2O + O2 | no activity with adenine | Enterobacter cloacae | 6-amino-7,9-dihydropurin-8-one + H2O2 | - |
? | |
| 1.17.3.2 | guanine + H2O + O2 | 53.3% of activity with xanthin | Enterobacter cloacae | 2-amino-7,9-dihydro-1H-purine-6,8-dione + H2O2 | - |
? | |
| 1.17.3.2 | hypoxanthine + 2 H2O + 2 O2 | 92.3% of activity with xanthin | Enterobacter cloacae | urate + 2 H2O2 | - |
? | |
| 1.17.3.2 | hypoxanthine + 2 H2O + 2 O2 | 92.3% of activity with xanthin | Enterobacter cloacae KY 3074 | urate + 2 H2O2 | - |
? | |
| 1.17.3.2 | purine + H2O + O2 | purine and derivatives | Enterobacter cloacae | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
| 1.17.3.2 | purine + H2O + O2 | 23.3% of activity with xanthine | Enterobacter cloacae | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
| 1.17.3.2 | purine + H2O + O2 | purine and derivatives | Enterobacter cloacae KY 3074 | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor O2 | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor ferricyanide | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor 2,6-dichlorophenolindophenol | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor O2 | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor ferricyanide | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? | |
| 1.17.3.2 | xanthine + H2O + O2 | electron acceptor 2,6-dichlorophenolindophenol | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.3.2 | dimer | 2 * 69000, SDS-PAGE | Enterobacter cloacae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 35 | - |
- |
Enterobacter cloacae |
| 1.17.3.2 | 37 | - |
assay at | Enterobacter cloacae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 5 | - |
5°C: about 10% of activity maximum, 60°C: about 50% of activity maximum | Enterobacter cloacae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 60 | - |
pH 6.2-6.8, 30 min, stable | Enterobacter cloacae |
| 1.17.3.2 | 70 | - |
30 min, complete loss of activity | Enterobacter cloacae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 7.1 | - |
- |
Enterobacter cloacae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 5 | 9 | pH 5.0: about 10% of activity maximum, pH 9.0: about 25% of activity maximum | Enterobacter cloacae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.3.2 | 6.2 | 6.8 | 60°C, 30 min | Enterobacter cloacae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.3.2 | FAD | flavoprotein | Enterobacter cloacae | |
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