Any feedback?
Literature summary extracted from
- Molecular and kinetic characterization of xanthine dehydrogenase from the phototrophic bacterium Rodopseudomonas capsulata (1981), Z. Naturforsch. C, 36, 933-941.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | 1-methylhypoxanthine | 17% inhibition of xanthine dehydrogenase at 0.25 mM | Rhodobacter capsulatus |  |
| 1.17.1.4 | 8-Azaadenine | complete inhibition of xanthine dehydrogenase at 0.2 mM | Rhodobacter capsulatus | |
| 1.17.1.4 | 8-Azaguanine | complete inhibition of xanthine dehydrogenase at 0.2 mM | Rhodobacter capsulatus | |
| 1.17.1.4 | 8-azohypoxanthine | 40% inhibition of xanthine dehydrogenase at 0.25 mM | Rhodobacter capsulatus | |
| 1.17.1.4 | adenine | competitive inhibition, Ki: 0.05 mM | Rhodobacter capsulatus | |
| 1.17.1.4 | Urate | competitive inhibition of xanthine dehydrogenase, Ki: 0.144 mM | Rhodobacter capsulatus | |
| 1.17.1.4 | xanthine | 40% inhibition of xanthine dehydrogenase at 0.25 mM | Rhodobacter capsulatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.4 | 0.0325 | - |
xanthine | - |
Rhodobacter capsulatus | |
| 1.17.1.4 | 0.0525 | - |
hypoxanthine | - |
Rhodobacter capsulatus | |
| 1.17.1.4 | 0.0612 | - |
NAD+ | - |
Rhodobacter capsulatus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | Fe | 8 atoms iron per mol enzyme | Rhodobacter capsulatus | |
| 1.17.1.4 | Mo | 2 molybdenum per mol enzyme | Rhodobacter capsulatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 84200 | - |
4 * 84200, SDS-PAGE | Rhodobacter capsulatus |
| 1.17.1.4 | 345000 | - |
gel filtration | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | hypoxanthine + NAD+ + H+ + O2- + H2O | Rhodobacter capsulatus | preferred substrate | xanthine + NADH + H2O2 | - |
ir | |
| 1.17.1.4 | xanthine + NAD+ + H2O | Rhodobacter capsulatus | 67% of the activity compared to hypoxanthine | urate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | Rhodobacter capsulatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.4 | to homogeneity, affinity chromatography | Rhodobacter capsulatus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | cell culture | inducible | Rhodobacter capsulatus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 35.9 | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | 1-methylhypoxanthine + NAD+ + H2O | 10% of the activity compared to hypoxanthine | Rhodobacter capsulatus | 1-methylxanthine + NADH | - |
? | |
| 1.17.1.4 | 1-methylxanthine + NAD+ + H2O | 10% of the activity compared to hypoxanthine | Rhodobacter capsulatus | 1-methylurate + NADH | - |
? | |
| 1.17.1.4 | 2-hydroxypurine + NAD+ + H2O | 35% of the activity compared to hypoxanthine, purine not oxidized | Rhodobacter capsulatus | ? + NADH | - |
? | |
| 1.17.1.4 | 6,8-dihydropurine + NAD+ + H2O | 50% of the activity compared to hypoxanthine | Rhodobacter capsulatus | ? + NADH | - |
? | |
| 1.17.1.4 | 8-azahypoxanthine + NAD+ + H2O | 39% 0f the activity compared to hypoxanthine | Rhodobacter capsulatus | 8-azaxanthine + NADH | - |
? | |
| 1.17.1.4 | hypoxanthine + NAD+ + H+ + O2- + H2O | preferred substrate | Rhodobacter capsulatus | xanthine + NADH + H2O2 | - |
ir | |
| 1.17.1.4 | NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Rhodobacter capsulatus | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| 1.17.1.4 | xanthine + NAD+ + H2O | 67% of the activity compared to hypoxanthine | Rhodobacter capsulatus | urate + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.1.4 | tetramer | 4 * 84200, SDS-PAGE | Rhodobacter capsulatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 37 | - |
highest activity, but unstable | Rhodobacter capsulatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 37 | - |
stable for 4 min | Rhodobacter capsulatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 8.3 | - |
- |
Rhodobacter capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | additional information | - |
Rhodobacter capsulatus | |
| 1.17.1.4 | NAD+ | cannot be replaced by NADP+ | Rhodobacter capsulatus | |
| 1.17.1.4 | NADH | - |
Rhodobacter capsulatus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
