BRENDA - Enzyme Database show

Regulation, purification, and properties of xanthine dehydrogenase in Neurospora crassa

Lyon, E.S.; Garrett, R.H.; J. Biol. Chem. 253, 2604-2614 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
2-amino-4-hydroxypterin
substrate inhibition above 0.01 mM
Neurospora crassa
1.17.1.4
2-Iodosobenzoic acid
effective inhibition of xanthine and pterine oxidation
Neurospora crassa
1.17.1.4
4-hydroxymercuribenzoate
effective inhibition of xanthine and pterine oxidation
Neurospora crassa
1.17.1.4
4-hydroxypyrazolo(3,4-d)pyrimidine
i.e. allopurinol; inhibition of pterine oxidation at 0.0003 mM
Neurospora crassa
1.17.1.4
adenine
effective inhibition of xanthine oxidation at 0.001 mM
Neurospora crassa
1.17.1.4
arsenite
inhibition of xanthine or pterine oxidation at 0.3 mM, diaphorase activity unaffected
Neurospora crassa
1.17.1.4
guanine
effective inhibition of xanthine oxidation at 0.001 mM
Neurospora crassa
1.17.1.4
KCN
35% inhibition of pterine oxidation, 60% inhibition of diaphorase activity at 5 mM
Neurospora crassa
1.17.1.4
NaN3
slight inhibition of dehydrogenase activity
Neurospora crassa
1.17.1.4
o-phenanthroline
50% inhibition at 5-15 mM
Neurospora crassa
1.17.1.4
Thiourea
65% inhibition of xanthine dehydrogenase activity at 20 mM
Neurospora crassa
1.17.1.4
Urate
inhibition of xanthine oxidation at 0.06 mM
Neurospora crassa
1.17.1.4
xanthine
substrate inhibition above 0.13 mM
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.0022
-
NAD+
oxidation of 2-amino-4-hydroxy-pterin
Neurospora crassa
1.17.1.4
0.0035
-
2-amino-4-hydroxypterin
-
Neurospora crassa
1.17.1.4
0.0082
-
xanthine
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
0.021
-
2,6-dichloroindophenol
diaphorase activity
Neurospora crassa
1.17.1.4
0.021
-
hypoxanthine
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
0.028
-
NAD+
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
6
-
NADH
diaphorase activity
Neurospora crassa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Fe
12.1 mol iron per mol enzyme
Neurospora crassa
1.17.1.4
Mo
1 mol molybdenum per mol enzyme
Neurospora crassa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
155000
-
2 * 155000, SDS-PAGE
Neurospora crassa
1.17.1.4
357000
-
sucrose density gradient centrifugation
Neurospora crassa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
2-amino-4-hydroxy-pterin + NAD+ + H2O
Neurospora crassa
i.e. pterin
isoxanthopterin + NADH
-
Neurospora crassa
r
1.17.1.4
hypoxanthine + NAD+ + H+ + O2- + H2O
Neurospora crassa
-
xanthine + NADH + H2O2
-
Neurospora crassa
r
1.17.1.4
xanthine + NAD+ + H2O
Neurospora crassa
degradative pathway of conversion of purines to ammonia
urate + NADH
-
Neurospora crassa
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.4
Neurospora crassa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, immunoabsorption chromatography
Neurospora crassa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
mycelium
inducible
Neurospora crassa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
0.152
-
-
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
2-amino-4-hydroxy-pterin + NAD+ + H2O
i.e. pterin
644604
Neurospora crassa
isoxanthopterin + NADH
-
644604
Neurospora crassa
r
1.17.1.4
2-amino-4-hydroxypterin + NAD+ + H2O
i.e. pterin
644604
Neurospora crassa
isoxanthopterin + NADH
-
644604
Neurospora crassa
r
1.17.1.4
hypoxanthine + NAD+ + H+ + O2- + H2O
-
644604
Neurospora crassa
xanthine + NADH + H2O2
-
644604
Neurospora crassa
r
1.17.1.4
NADH + electron acceptor + H2O
2,6-dichloroindophenol as electron acceptor
644604
Neurospora crassa
NAD+ + reduced electron acceptor
-
644604
Neurospora crassa
?
1.17.1.4
xanthine + NAD+ + H2O
degradative pathway of conversion of purines to ammonia
644604
Neurospora crassa
urate + NADH
-
644604
Neurospora crassa
r
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.4
dimer
2 * 155000, SDS-PAGE
Neurospora crassa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
2 mol per mol enzyme
Neurospora crassa
1.17.1.4
additional information
non-heme flavoprotein
Neurospora crassa
1.17.1.4
NAD+
cannot be replaced by NADP+
Neurospora crassa
1.17.1.4
NADH
-
Neurospora crassa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
2 mol per mol enzyme
Neurospora crassa
1.17.1.4
additional information
non-heme flavoprotein
Neurospora crassa
1.17.1.4
NAD+
cannot be replaced by NADP+
Neurospora crassa
1.17.1.4
NADH
-
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
2-amino-4-hydroxypterin
substrate inhibition above 0.01 mM
Neurospora crassa
1.17.1.4
2-Iodosobenzoic acid
effective inhibition of xanthine and pterine oxidation
Neurospora crassa
1.17.1.4
4-hydroxymercuribenzoate
effective inhibition of xanthine and pterine oxidation
Neurospora crassa
1.17.1.4
4-hydroxypyrazolo(3,4-d)pyrimidine
i.e. allopurinol; inhibition of pterine oxidation at 0.0003 mM
Neurospora crassa
1.17.1.4
adenine
effective inhibition of xanthine oxidation at 0.001 mM
Neurospora crassa
1.17.1.4
arsenite
inhibition of xanthine or pterine oxidation at 0.3 mM, diaphorase activity unaffected
Neurospora crassa
1.17.1.4
guanine
effective inhibition of xanthine oxidation at 0.001 mM
Neurospora crassa
1.17.1.4
KCN
35% inhibition of pterine oxidation, 60% inhibition of diaphorase activity at 5 mM
Neurospora crassa
1.17.1.4
NaN3
slight inhibition of dehydrogenase activity
Neurospora crassa
1.17.1.4
o-phenanthroline
50% inhibition at 5-15 mM
Neurospora crassa
1.17.1.4
Thiourea
65% inhibition of xanthine dehydrogenase activity at 20 mM
Neurospora crassa
1.17.1.4
Urate
inhibition of xanthine oxidation at 0.06 mM
Neurospora crassa
1.17.1.4
xanthine
substrate inhibition above 0.13 mM
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.0022
-
NAD+
oxidation of 2-amino-4-hydroxy-pterin
Neurospora crassa
1.17.1.4
0.0035
-
2-amino-4-hydroxypterin
-
Neurospora crassa
1.17.1.4
0.0082
-
xanthine
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
0.021
-
2,6-dichloroindophenol
diaphorase activity
Neurospora crassa
1.17.1.4
0.021
-
hypoxanthine
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
0.028
-
NAD+
xanthine dehydrogenase activity
Neurospora crassa
1.17.1.4
6
-
NADH
diaphorase activity
Neurospora crassa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Fe
12.1 mol iron per mol enzyme
Neurospora crassa
1.17.1.4
Mo
1 mol molybdenum per mol enzyme
Neurospora crassa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
155000
-
2 * 155000, SDS-PAGE
Neurospora crassa
1.17.1.4
357000
-
sucrose density gradient centrifugation
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
2-amino-4-hydroxy-pterin + NAD+ + H2O
Neurospora crassa
i.e. pterin
isoxanthopterin + NADH
-
Neurospora crassa
r
1.17.1.4
hypoxanthine + NAD+ + H+ + O2- + H2O
Neurospora crassa
-
xanthine + NADH + H2O2
-
Neurospora crassa
r
1.17.1.4
xanthine + NAD+ + H2O
Neurospora crassa
degradative pathway of conversion of purines to ammonia
urate + NADH
-
Neurospora crassa
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, immunoabsorption chromatography
Neurospora crassa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
mycelium
inducible
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
0.152
-
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
2-amino-4-hydroxy-pterin + NAD+ + H2O
i.e. pterin
644604
Neurospora crassa
isoxanthopterin + NADH
-
644604
Neurospora crassa
r
1.17.1.4
2-amino-4-hydroxypterin + NAD+ + H2O
i.e. pterin
644604
Neurospora crassa
isoxanthopterin + NADH
-
644604
Neurospora crassa
r
1.17.1.4
hypoxanthine + NAD+ + H+ + O2- + H2O
-
644604
Neurospora crassa
xanthine + NADH + H2O2
-
644604
Neurospora crassa
r
1.17.1.4
NADH + electron acceptor + H2O
2,6-dichloroindophenol as electron acceptor
644604
Neurospora crassa
NAD+ + reduced electron acceptor
-
644604
Neurospora crassa
?
1.17.1.4
xanthine + NAD+ + H2O
degradative pathway of conversion of purines to ammonia
644604
Neurospora crassa
urate + NADH
-
644604
Neurospora crassa
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.4
dimer
2 * 155000, SDS-PAGE
Neurospora crassa