BRENDA - Enzyme Database show

Purification and properties of a novel ferricyanide-linked xanthine dehydrogenase from Pseudomonas putida 40

Woolfolk, C.A.; J. Bacteriol. 163, 600-609 (1985)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.17.1.4
oxygen-linked activity rather unstable during storage at 4░C, ferricyanide-linked activity stable to freezing and thawing
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
arsenite
90% loss of the ferricyanide-linked activity in the presence of 1.78 mM
Pseudomonas putida
1.17.1.4
KCN
complete inactivation of oxygen-linked activity in 15 min, decline of NAD+-linked activity in 75 min, ferricyanide-linked activity completely stable
Pseudomonas putida
1.17.1.4
methanol
50% inhibition in 3 min at 1.5 M
Pseudomonas putida
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Mo
-
Pseudomonas putida
1.17.1.4
molybdenum-center
-
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
72000
-
x * 72000, SDS-PAGE
Pseudomonas putida
1.17.1.4
255000
-
sucrose density gradient centrifugation, gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
xanthine + NAD+ + H2O
Pseudomonas putida
NAD+-linked activity, very low activity towards molecular oxygen
urate + NADH
-
Pseudomonas putida
?
1.17.1.4
xanthine + O2 + H2O
Pseudomonas putida
very low activity
urate + O2- + 2 H+
-
Pseudomonas putida
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.17.1.4
Methanol
sensitive to, half-life: 3 min at 1.5 M
Pseudomonas putida
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.4
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, mainly ferricyanide- and NAD+-linked activities, chromatography steps
Pseudomonas putida
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
0.0025 mg/ml sodium tungstate in the growth medium reduces level of ferricyanide-linked enzyme to 3%
Pseudomonas putida
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.1.4
163
-
ferricyanide-linked activity
Pseudomonas putida
Storage Stability
EC Number
Storage Stability
Organism
1.17.1.4
4░C, 1 week
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
11% of the activity compared to ferricyanide as electron acceptor
644593
Pseudomonas putida
urate + ?
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + ferricyanide + H2O
specific ferricyanide-dependent activity, no activity with NAD+, NADP+, oxygen, cytochrome c, FAD or FMN
644593
Pseudomonas putida
urate + ferrocyanide
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + NAD+ + H2O
NAD+-linked activity, very low activity towards molecular oxygen
644593
Pseudomonas putida
urate + NADH
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + O2 + H2O
very low activity
644593
Pseudomonas putida
urate + O2- + 2 H+
-
644593
Pseudomonas putida
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.4
?
x * 72000, SDS-PAGE
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
ferricyanide
specific xanthine oxidizing activity
Pseudomonas putida
1.17.1.4
heme
ferricyanide-linked activity, no FAD
Pseudomonas putida
1.17.1.4
NAD+
activity different from ferricyanide-linked activity
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
ferricyanide
specific xanthine oxidizing activity
Pseudomonas putida
1.17.1.4
heme
ferricyanide-linked activity, no FAD
Pseudomonas putida
1.17.1.4
NAD+
activity different from ferricyanide-linked activity
Pseudomonas putida
General Stability (protein specific)
EC Number
General Stability
Organism
1.17.1.4
oxygen-linked activity rather unstable during storage at 4░C, ferricyanide-linked activity stable to freezing and thawing
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
arsenite
90% loss of the ferricyanide-linked activity in the presence of 1.78 mM
Pseudomonas putida
1.17.1.4
KCN
complete inactivation of oxygen-linked activity in 15 min, decline of NAD+-linked activity in 75 min, ferricyanide-linked activity completely stable
Pseudomonas putida
1.17.1.4
methanol
50% inhibition in 3 min at 1.5 M
Pseudomonas putida
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Mo
-
Pseudomonas putida
1.17.1.4
molybdenum-center
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
72000
-
x * 72000, SDS-PAGE
Pseudomonas putida
1.17.1.4
255000
-
sucrose density gradient centrifugation, gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
xanthine + NAD+ + H2O
Pseudomonas putida
NAD+-linked activity, very low activity towards molecular oxygen
urate + NADH
-
Pseudomonas putida
?
1.17.1.4
xanthine + O2 + H2O
Pseudomonas putida
very low activity
urate + O2- + 2 H+
-
Pseudomonas putida
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.17.1.4
Methanol
sensitive to, half-life: 3 min at 1.5 M
Pseudomonas putida
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, mainly ferricyanide- and NAD+-linked activities, chromatography steps
Pseudomonas putida
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
0.0025 mg/ml sodium tungstate in the growth medium reduces level of ferricyanide-linked enzyme to 3%
Pseudomonas putida
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.1.4
163
-
ferricyanide-linked activity
Pseudomonas putida
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.17.1.4
4░C, 1 week
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
11% of the activity compared to ferricyanide as electron acceptor
644593
Pseudomonas putida
urate + ?
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + ferricyanide + H2O
specific ferricyanide-dependent activity, no activity with NAD+, NADP+, oxygen, cytochrome c, FAD or FMN
644593
Pseudomonas putida
urate + ferrocyanide
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + NAD+ + H2O
NAD+-linked activity, very low activity towards molecular oxygen
644593
Pseudomonas putida
urate + NADH
-
644593
Pseudomonas putida
?
1.17.1.4
xanthine + O2 + H2O
very low activity
644593
Pseudomonas putida
urate + O2- + 2 H+
-
644593
Pseudomonas putida
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.4
?
x * 72000, SDS-PAGE
Pseudomonas putida