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Literature summary extracted from

  • Woolfolk, C.A.
    Purification and properties of a novel ferricyanide-linked xanthine dehydrogenase from Pseudomonas putida 40 (1985), J. Bacteriol., 163, 600-609.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.17.1.4 oxygen-linked activity rather unstable during storage at 4°C, ferricyanide-linked activity stable to freezing and thawing Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 arsenite 90% loss of the ferricyanide-linked activity in the presence of 1.78 mM Pseudomonas putida
1.17.1.4 KCN complete inactivation of oxygen-linked activity in 15 min, decline of NAD+-linked activity in 75 min, ferricyanide-linked activity completely stable Pseudomonas putida
1.17.1.4 methanol 50% inhibition in 3 min at 1.5 M Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Mo
-
 Pseudomonas putida
1.17.1.4 molybdenum-center
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 72000
-
 x * 72000, SDS-PAGE Pseudomonas putida
1.17.1.4 255000
-
 sucrose density gradient centrifugation, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Pseudomonas putida NAD+-linked activity, very low activity towards molecular oxygen urate + NADH
-
 ?
1.17.1.4 xanthine + O2 + H2O Pseudomonas putida very low activity urate + O2- + 2 H+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.17.1.4 Methanol sensitive to, half-life: 3 min at 1.5 M Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, mainly ferricyanide- and NAD+-linked activities, chromatography steps Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture 0.0025 mg/ml sodium tungstate in the growth medium reduces level of ferricyanide-linked enzyme to 3% Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 163
-
 ferricyanide-linked activity Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.17.1.4 4°C, 1 week Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O 11% of the activity compared to ferricyanide as electron acceptor Pseudomonas putida urate + ?
-
 ?
1.17.1.4 xanthine + ferricyanide + H2O specific ferricyanide-dependent activity, no activity with NAD+, NADP+, oxygen, cytochrome c, FAD or FMN Pseudomonas putida urate + ferrocyanide
-
 ?
1.17.1.4 xanthine + NAD+ + H2O NAD+-linked activity, very low activity towards molecular oxygen Pseudomonas putida urate + NADH
-
 ?
1.17.1.4 xanthine + O2 + H2O very low activity Pseudomonas putida urate + O2- + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? x * 72000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 ferricyanide specific xanthine oxidizing activity Pseudomonas putida
1.17.1.4 heme ferricyanide-linked activity, no FAD Pseudomonas putida
1.17.1.4 NAD+ activity different from ferricyanide-linked activity Pseudomonas putida