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Literature summary extracted from

  • Waud, W.R.; Rajagopalan, K.V.
    The mechanism of conversion of rat liver xanthine dehydrogenase from an NAD+-dependent form (type D) to an O2-dependent form (type O) (1976), Arch. Biochem. Biophys., 172, 365-379.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.4 DTT recovery from oxidase to dehydrogenase type Bos taurus
1.17.1.4 DTT recovery from oxidase to dehydrogenase type Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 5,5-dithiobis-(2-nitrobenzoate) conversion of dehydrogenase type D to oxidase type O due to modification of a limited number of critical sulfhydryl groups Rattus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Mo
-
Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 150000
-
x * 150000, SDS-urea -PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Bos taurus NAD+-dependent dehydrogenase type D urate + NADH
-
?
1.17.1.4 xanthine + NAD+ + H2O Rattus sp. NAD+-dependent dehydrogenase type D urate + NADH
-
?
1.17.1.4 xanthine + NAD+ + O2 + H2O + H+ Rattus sp. heat-treated intermediate dehydrogenase/oxidase type O urate + NADH + H2O2
-
?
1.17.1.4 xanthine + O2 + H2O Bos taurus
-
urate + O2- + 2 H+
-
?
1.17.1.4 xanthine + O2 + H2O Rattus sp. NAD+-independent trypsin-treated oxidase type O urate + O2- + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Bos taurus
-
-
-
1.17.1.4 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 purified as antibody complex Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 liver
-
Rattus sp.
-
1.17.1.4 milk
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 NADH + electron acceptor + H2O conversion to the oxidase type O by trypsinization leads to 80-100% decrease in the oxidation rate of NADH, conversion to the oxidase type O by heat-treatment leads to a diminution of NADH oxidation Rattus sp. NAD+ + reduced electron acceptor
-
?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O
-
Bos taurus urate + ?
-
?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O greatly enhanced activity for dehydrogenase type D and trypsin- or heat-treated oxidase types O Rattus sp. urate + ?
-
?
1.17.1.4 xanthine + cytochrome c + H2O enhanced activity for heat- and trypsin-treated oxidase types O Rattus sp. urate + reduced cytochrome c
-
?
1.17.1.4 xanthine + ferricyanide + H2O low activity for both dehydrogenase type D and oxidase type O Rattus sp. urate + ferrocyanide
-
?
1.17.1.4 xanthine + methylene blue + H2O enhanced oxidation of xanthine for dehydrogenase type D and trypsin- or heat-treated oxidase type O Rattus sp. urate + reduced methylene blue
-
?
1.17.1.4 xanthine + NAD+ + H2O NAD+-dependent dehydrogenase type D Bos taurus urate + NADH
-
?
1.17.1.4 xanthine + NAD+ + H2O NAD+-dependent dehydrogenase type D Rattus sp. urate + NADH
-
?
1.17.1.4 xanthine + NAD+ + O2 + H2O + H+ heat-treated intermediate dehydrogenase/oxidase type O Rattus sp. urate + NADH + H2O2
-
?
1.17.1.4 xanthine + O2 + H2O
-
Bos taurus urate + O2- + 2 H+
-
?
1.17.1.4 xanthine + O2 + H2O NAD+-independent trypsin-treated oxidase type O Rattus sp. urate + O2- + 2 H+
-
?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? x * 150000, SDS-urea -PAGE Rattus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.4 37
-
conversion to an intermediate dehydrogenase-oxidase type O form in 30 min Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
Bos taurus
1.17.1.4 FAD trypsin-treated oxidase type O shows absence of FAD, heat-treated oxidase type O shows small measurable FAD Rattus sp.
1.17.1.4 NAD+
-
Bos taurus
1.17.1.4 NAD+
-
Rattus sp.