EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.4 | dithioerythritol | recovery from oxidase to dehydrogenase type | Rattus sp. |
EC Number | General Stability | Organism |
---|---|---|
1.17.3.2 | the enzyme from animal tissues can be interconverted to EC 1.1.1.204, that from liver exists in vivo mainly as the dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.4 | 5,5-dithiobis-(2-nitrobenzoate) | conversion of dehydrogenase type D to oxidase type O, can be prevented and reversed by dithioerythritol | Rattus sp. | |
1.17.1.4 | CuSO4 | conversion of the dehydrogenase type D to oxidase type O, prolonged incubation leads to complete inactivation, conversion can be reversed and prevented by dithioerythritol | Rattus sp. | |
1.17.1.4 | N-ethylmaleimide | conversion of dehydrogenase type D to oxidase type O, prevented by dithioerythritol but no reversible conversion | Rattus sp. | |
1.17.1.4 | Tetraethylthiuram disulfide | conversion of dehydrogenase type D to oxidase type O, can be prevented and reversed by dithioerythritol | Rattus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.3.2 | Iron | iron-molybdenum protein | Rattus norvegicus | |
1.17.3.2 | Molybdenum | an iron-molybdenum protein | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.4 | xanthine + NAD+ + H2O | Rattus sp. | - |
urate + NADH | - |
? | |
1.17.1.4 | xanthine + NAD+ + H2O | Gallus gallus | only dehydrogenase type D present | urate + NADH | - |
? | |
1.17.1.4 | xanthine + NAD+ + O2 + H2O + H+ | Rattus sp. | intermediate form of dehydrogenase/oxidase type D/O | urate + NADH + H2O2 | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | Gallus gallus | - |
urate + O2- + 2 H+ | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | Rattus sp. | - |
urate + O2- + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.4 | Gallus gallus | - |
chicken | - |
1.17.1.4 | no activity in Columba livia | - |
- |
- |
1.17.1.4 | Rattus sp. | - |
- |
- |
1.17.3.2 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.17.3.2 | additional information | the enzyme from animal tissues can be interconverted to xanthine dehydrogenase, EC 1.1.1.204, the liver enzyme exists in vivo mainly in its dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol | Rattus norvegicus |
1.17.3.2 | proteolytic modification | - |
Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.4 | during purification conversion from dehydrogenase type D to oxidase type O, reversible by dithioerythritol | Rattus sp. |
1.17.3.2 | ammonium sulfate, hydroxyapatite, DEAE-cellulose | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.17.1.4 | heart | only oxidase type O form | Rattus sp. | - |
1.17.1.4 | intestine | only oxidase type O form, can be converted to dehydrogenase type D by dithioerythritol | Rattus sp. | - |
1.17.1.4 | kidney | only oxidase type O form | Rattus sp. | - |
1.17.1.4 | liver | - |
Gallus gallus | - |
1.17.1.4 | liver | dehydrogenase and oxidase forms | Rattus sp. | - |
1.17.1.4 | lung | only oxidase type O form | Rattus sp. | - |
1.17.1.4 | pancreas | only oxidase type O form | Rattus sp. | - |
1.17.1.4 | spleen | only oxidase type O form | Rattus sp. | - |
1.17.3.2 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 189.6 | - |
electron acceptor O2 | Rattus norvegicus |
1.17.3.2 | 800 | - |
electron acceptors O2 and NAD+ | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.4 | xanthine + NAD+ + H2O | - |
Rattus sp. | urate + NADH | - |
? | |
1.17.1.4 | xanthine + NAD+ + H2O | only dehydrogenase type D present | Gallus gallus | urate + NADH | - |
? | |
1.17.1.4 | xanthine + NAD+ + O2 + H2O + H+ | intermediate form of dehydrogenase/oxidase type D/O | Rattus sp. | urate + NADH + H2O2 | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | - |
Gallus gallus | urate + O2- + 2 H+ | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | - |
Rattus sp. | urate + O2- + 2 H+ | - |
? | |
1.17.3.2 | hypoxanthine + 2 H2O + 2 O2 | - |
Rattus norvegicus | urate + 2 H2O2 | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | electron acceptor O2 | Rattus norvegicus | uric acid + H2O2 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.4 | -20 | - |
conversion of dehydrogenase type D to oxidase type O | Rattus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.4 | NAD+ | - |
Gallus gallus | |
1.17.1.4 | NAD+ | - |
Rattus sp. | |
1.17.3.2 | FAD | flavoprotein | Rattus norvegicus |