BRENDA - Enzyme Database show

Purification and properties of chicken liver xanthine dehydrogenase

Rajagopalan, K.V.; Handler, P.; J. Biol. Chem. 242, 4097-4107 (1967)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
ammeline
competitive inhibition of xanthine oxidation Ki: 0.083 mM, uncompetitive inhibition of NADH oxidation Ki: 0.063 mM
Gallus gallus
1.17.1.4
arsenite
gradual inhibition of xanthine-2,6-dichloroindophenol-activity, paralleled by a corresponding increase of NADH-2,6-dichloroindophenol-activity
Gallus gallus
1.17.1.4
KCN
complete inhibition of xanthine dehydrogenase activity, 70% reduction of diaphorase activity
Gallus gallus
1.17.1.4
methanol
slight inhibition of NAD+ reduction at 1.5 M, rapid inactivation if NAD+ is replaced by 2,6-dichloroindophenol, enhanced NADH diaphorase activity
Gallus gallus
1.17.1.4
NAD+
competitive inhibition of NADH oxidation, Ki: 0.0143 mM
Gallus gallus
1.17.1.4
Urea
competitive inhibition of xanthine oxidation Ki: 0.28 M, uncompetitive inhibition of NADH oxidation Ki: 1 M
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Fe
-
Gallus gallus
1.17.1.4
Mo
-
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
300000
-
sedimentation equilibrium measurements
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
xanthine + NAD+ + H2O
Gallus gallus
ping-pong reaction mechanism
urate + NADH
-
Gallus gallus
r
1.17.1.4
xanthine + O2 + H2O
Gallus gallus
low activity
urate + O2- + 2 H+
-
Gallus gallus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.4
Gallus gallus
-
chicken
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, chromatography techniques
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
liver
-
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
NADH + electron acceptor + H2O
NADH diaphorase activity with several acceptors
644579
Gallus gallus
NAD+ + reduced electron acceptor
-
644579
Gallus gallus
?
1.17.1.4
NADH + electron acceptor + H2O
2,6-dichloroindophenol, 3-acetylpyridine-adenine dinucleotide, methylene blue, phenazine methosulfate or trinitrobenzene sulfonate
644579
Gallus gallus
NAD+ + reduced electron acceptor
-
644579
Gallus gallus
?
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
same activity compared to NAD+ as electron acceptor
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r
1.17.1.4
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O
same activity compared to NAD+
644579
Gallus gallus
urate + 3-acetylpyridine-adenine dinucleotide(H)
-
644579
Gallus gallus
r
1.17.1.4
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O
low reverse activity
644579
Gallus gallus
urate + 3-acetylpyridine-adenine dinucleotide(H)
-
644579
Gallus gallus
r
1.17.1.4
xanthine + methylene blue + H2O
-
644579
Gallus gallus
urate + reduced methylene blue
-
644579
Gallus gallus
r
1.17.1.4
xanthine + NAD+ + H2O
ping-pong reaction mechanism
644579
Gallus gallus
urate + NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + O2 + H2O
low activity
644579
Gallus gallus
urate + O2- + 2 H+
-
644579
Gallus gallus
r
1.17.1.4
xanthine + phenazine methosulfate + H2O
-
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r
1.17.1.4
xanthine + pyridinealdehyde-NAD+ + H2O
53% of the activity compared to NAD+, low reverse activity
644579
Gallus gallus
urate + pyridinealdehyde-NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + thio-NAD+ + H2O
same activity compared to NAD+
644579
Gallus gallus
urate + thio-NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + trinitrobenzenesulfonate + H2O
-
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Gallus gallus
1.17.1.4
additional information
molybdoironflavoprotein: 1 : 1 : 4 ratio of molybdenum to FAD to iron
Gallus gallus
1.17.1.4
NAD+
-
Gallus gallus
1.17.1.4
NADH
-
Gallus gallus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Gallus gallus
1.17.1.4
additional information
molybdoironflavoprotein: 1 : 1 : 4 ratio of molybdenum to FAD to iron
Gallus gallus
1.17.1.4
NAD+
-
Gallus gallus
1.17.1.4
NADH
-
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
ammeline
competitive inhibition of xanthine oxidation Ki: 0.083 mM, uncompetitive inhibition of NADH oxidation Ki: 0.063 mM
Gallus gallus
1.17.1.4
arsenite
gradual inhibition of xanthine-2,6-dichloroindophenol-activity, paralleled by a corresponding increase of NADH-2,6-dichloroindophenol-activity
Gallus gallus
1.17.1.4
KCN
complete inhibition of xanthine dehydrogenase activity, 70% reduction of diaphorase activity
Gallus gallus
1.17.1.4
methanol
slight inhibition of NAD+ reduction at 1.5 M, rapid inactivation if NAD+ is replaced by 2,6-dichloroindophenol, enhanced NADH diaphorase activity
Gallus gallus
1.17.1.4
NAD+
competitive inhibition of NADH oxidation, Ki: 0.0143 mM
Gallus gallus
1.17.1.4
Urea
competitive inhibition of xanthine oxidation Ki: 0.28 M, uncompetitive inhibition of NADH oxidation Ki: 1 M
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Fe
-
Gallus gallus
1.17.1.4
Mo
-
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
300000
-
sedimentation equilibrium measurements
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
xanthine + NAD+ + H2O
Gallus gallus
ping-pong reaction mechanism
urate + NADH
-
Gallus gallus
r
1.17.1.4
xanthine + O2 + H2O
Gallus gallus
low activity
urate + O2- + 2 H+
-
Gallus gallus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, chromatography techniques
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
liver
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
NADH + electron acceptor + H2O
NADH diaphorase activity with several acceptors
644579
Gallus gallus
NAD+ + reduced electron acceptor
-
644579
Gallus gallus
?
1.17.1.4
NADH + electron acceptor + H2O
2,6-dichloroindophenol, 3-acetylpyridine-adenine dinucleotide, methylene blue, phenazine methosulfate or trinitrobenzene sulfonate
644579
Gallus gallus
NAD+ + reduced electron acceptor
-
644579
Gallus gallus
?
1.17.1.4
xanthine + 2,6-dichlorophenolindophenol + H2O
same activity compared to NAD+ as electron acceptor
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r
1.17.1.4
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O
same activity compared to NAD+
644579
Gallus gallus
urate + 3-acetylpyridine-adenine dinucleotide(H)
-
644579
Gallus gallus
r
1.17.1.4
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O
low reverse activity
644579
Gallus gallus
urate + 3-acetylpyridine-adenine dinucleotide(H)
-
644579
Gallus gallus
r
1.17.1.4
xanthine + methylene blue + H2O
-
644579
Gallus gallus
urate + reduced methylene blue
-
644579
Gallus gallus
r
1.17.1.4
xanthine + NAD+ + H2O
ping-pong reaction mechanism
644579
Gallus gallus
urate + NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + O2 + H2O
low activity
644579
Gallus gallus
urate + O2- + 2 H+
-
644579
Gallus gallus
r
1.17.1.4
xanthine + phenazine methosulfate + H2O
-
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r
1.17.1.4
xanthine + pyridinealdehyde-NAD+ + H2O
53% of the activity compared to NAD+, low reverse activity
644579
Gallus gallus
urate + pyridinealdehyde-NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + thio-NAD+ + H2O
same activity compared to NAD+
644579
Gallus gallus
urate + thio-NADH
-
644579
Gallus gallus
r
1.17.1.4
xanthine + trinitrobenzenesulfonate + H2O
-
644579
Gallus gallus
urate + ?
-
644579
Gallus gallus
r