BRENDA - Enzyme Database show

Selenium-containing xanthine dehydrogenase from Eubacterium barkeri

Schräder, T.; Rienhöfer, A.; Andreesen, J.R.; Eur. J. Biochem. 264, 862-871 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
4-hydroxypyrazolo(3,4-d)pyrimidine
95% inhibition of hypoxanthine-NADP+-activity at 1 mM; i.e. allopurinol
Eubacterium barkeri
1.17.1.4
6-Mercaptopurine
90% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
adenine
96% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
arsenite
50% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
KCN
addition of selenide in the presence of dithionite reactivates the inhibited enzyme
Eubacterium barkeri
1.17.1.4
Sodium dithionite
reduction of enzyme
Eubacterium barkeri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.038
-
NADP+
electron donor: hypoxanthine
Eubacterium barkeri
1.17.1.4
0.067
-
xanthine
electron acceptor: NADP+
Eubacterium barkeri
1.17.1.4
0.21
-
hypoxanthine
electron acceptor: NADP+
Eubacterium barkeri
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Iron-sulfur-center
2 Fe2-S2-clusters
Eubacterium barkeri
1.17.1.4
molybdenum-center
-
Eubacterium barkeri
1.17.1.4
Se
3.4 g-atom per mol enzyme
Eubacterium barkeri
1.17.1.4
Tungsten
3.4 g-atom per mol of enzyme
Eubacterium barkeri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
17500
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
30000
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
81300
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
530000
-
gel filtration
Eubacterium barkeri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
hypoxanthine + NADP+ + H2O
Eubacterium barkeri
strict specificity for NADP+
xanthine + NADPH
-
Eubacterium barkeri
?
1.17.1.4
NADPH + O2
Eubacterium barkeri
high NADPH oxidase activity
NADP+ + O2- + H+
-
Eubacterium barkeri
?
1.17.1.4
purine + NADP+ + H2O
Eubacterium barkeri
60% of the activity compared to hypoxanthine
? + NADPH
-
Eubacterium barkeri
?
1.17.1.4
xanthine + NADP+ + H2O
Eubacterium barkeri
strict specificity for NADP+
urate + NADPH
-
Eubacterium barkeri
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.4
Eubacterium barkeri
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, chromatography techniques
Eubacterium barkeri
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
inducible by xanthine, selenite and molybdate
Eubacterium barkeri
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
164
-
hypoxanthine-NADP+-assay
Eubacterium barkeri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
4-aminoimidazole-5-carboxamide + NADP+ + H2O
38% of the activity compared to hypoxanthine-NADP+
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
hypoxanthine + NADP+ + H2O
strict specificity for NADP+
644566
Eubacterium barkeri
xanthine + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
N-methylnicontinamide + NADP+ + H2O
low activity, only a substrate at pH values above 8.0
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
NADPH + electron acceptor + H2O
electron acceptors: 2,6-dichlorophenolindophenol, methyl viologen, benzyl viologen, methylene blue
644566
Eubacterium barkeri
NADP+ + reduced electron acceptor
-
644566
Eubacterium barkeri
?
1.17.1.4
NADPH + O2
high NADPH oxidase activity
644566
Eubacterium barkeri
NADP+ + O2- + H+
-
644566
Eubacterium barkeri
?
1.17.1.4
purine + NADP+ + H2O
60% of the activity compared to hypoxanthine
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
xanthine + NADP+ + H2O
strict specificity for NADP+
644566
Eubacterium barkeri
urate + NADPH
-
644566
Eubacterium barkeri
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.4
dodecamer
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Eubacterium barkeri
1.17.1.4
additional information
molybdoironflavoprotein: 17.5 mol iron, 18.4 mol acid-labile sulfur, 2.3 mol molybdenum, 1.1 mol tungsten, 0.95 mol selenium
Eubacterium barkeri
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
FAD
-
Eubacterium barkeri
1.17.1.4
additional information
molybdoironflavoprotein: 17.5 mol iron, 18.4 mol acid-labile sulfur, 2.3 mol molybdenum, 1.1 mol tungsten, 0.95 mol selenium
Eubacterium barkeri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.4
4-hydroxypyrazolo(3,4-d)pyrimidine
95% inhibition of hypoxanthine-NADP+-activity at 1 mM; i.e. allopurinol
Eubacterium barkeri
1.17.1.4
6-Mercaptopurine
90% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
adenine
96% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
arsenite
50% inhibition of hypoxanthine-NADP+-activity at 1 mM
Eubacterium barkeri
1.17.1.4
KCN
addition of selenide in the presence of dithionite reactivates the inhibited enzyme
Eubacterium barkeri
1.17.1.4
Sodium dithionite
reduction of enzyme
Eubacterium barkeri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.4
0.038
-
NADP+
electron donor: hypoxanthine
Eubacterium barkeri
1.17.1.4
0.067
-
xanthine
electron acceptor: NADP+
Eubacterium barkeri
1.17.1.4
0.21
-
hypoxanthine
electron acceptor: NADP+
Eubacterium barkeri
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.1.4
Iron-sulfur-center
2 Fe2-S2-clusters
Eubacterium barkeri
1.17.1.4
molybdenum-center
-
Eubacterium barkeri
1.17.1.4
Se
3.4 g-atom per mol enzyme
Eubacterium barkeri
1.17.1.4
Tungsten
3.4 g-atom per mol of enzyme
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.4
17500
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
30000
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
81300
-
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri
1.17.1.4
530000
-
gel filtration
Eubacterium barkeri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.4
hypoxanthine + NADP+ + H2O
Eubacterium barkeri
strict specificity for NADP+
xanthine + NADPH
-
Eubacterium barkeri
?
1.17.1.4
NADPH + O2
Eubacterium barkeri
high NADPH oxidase activity
NADP+ + O2- + H+
-
Eubacterium barkeri
?
1.17.1.4
purine + NADP+ + H2O
Eubacterium barkeri
60% of the activity compared to hypoxanthine
? + NADPH
-
Eubacterium barkeri
?
1.17.1.4
xanthine + NADP+ + H2O
Eubacterium barkeri
strict specificity for NADP+
urate + NADPH
-
Eubacterium barkeri
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.4
to homogeneity, chromatography techniques
Eubacterium barkeri
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.17.1.4
cell culture
inducible by xanthine, selenite and molybdate
Eubacterium barkeri
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.1.4
164
-
hypoxanthine-NADP+-assay
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.4
4-aminoimidazole-5-carboxamide + NADP+ + H2O
38% of the activity compared to hypoxanthine-NADP+
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
hypoxanthine + NADP+ + H2O
strict specificity for NADP+
644566
Eubacterium barkeri
xanthine + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
N-methylnicontinamide + NADP+ + H2O
low activity, only a substrate at pH values above 8.0
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
NADPH + electron acceptor + H2O
electron acceptors: 2,6-dichlorophenolindophenol, methyl viologen, benzyl viologen, methylene blue
644566
Eubacterium barkeri
NADP+ + reduced electron acceptor
-
644566
Eubacterium barkeri
?
1.17.1.4
NADPH + O2
high NADPH oxidase activity
644566
Eubacterium barkeri
NADP+ + O2- + H+
-
644566
Eubacterium barkeri
?
1.17.1.4
purine + NADP+ + H2O
60% of the activity compared to hypoxanthine
644566
Eubacterium barkeri
? + NADPH
-
644566
Eubacterium barkeri
?
1.17.1.4
xanthine + NADP+ + H2O
strict specificity for NADP+
644566
Eubacterium barkeri
urate + NADPH
-
644566
Eubacterium barkeri
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.4
dodecamer
tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE
Eubacterium barkeri