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Literature summary extracted from
- On a non-pyridine nucleotide-dependent 2-oxoacid reductase of broad specificity from two Proteus species (1985), FEBS Lett., 167, 29-32.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.99.30 | synthesis | enzyme can be used as a stereospecific biocatalyst for production of a wide range of 2-hydroxy-carboxylate compounds at preparative scale, with cofactor methyl or benzyl viologen being more stable than NAD(P)H | Proteus mirabilis |
| 1.1.99.30 | synthesis | enzyme can be used as a stereospecific biocatalyst for production of a wide range of 2-hydroxy-carboxylate compounds at preparative scale, with cofactor methyl or benzyl viologen being more stable than NAD(P)H | Proteus vulgaris |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.30 | CN- | 30% inhibition at 10 mM | Proteus mirabilis |  |
| 1.1.99.30 | CN- | 30% inhibition at 10 mM | Proteus vulgaris | |
| 1.1.99.30 | additional information | no inhibition by CO | Proteus mirabilis | |
| 1.1.99.30 | additional information | no inhibition by CO | Proteus vulgaris |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.30 | membrane | bound | Proteus mirabilis | 16020 | - |
| 1.1.99.30 | membrane | bound | Proteus vulgaris | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.30 | Molybdenum | dependent on | Proteus mirabilis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.30 | Proteus mirabilis | - |
- |
- |
| 1.1.99.30 | Proteus sp. | - |
- |
- |
| 1.1.99.30 | Proteus vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.30 | - |
Proteus vulgaris |
| 1.1.99.30 | 167fold | Proteus mirabilis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.99.30 | additional information | aerobic growth conditions | Proteus mirabilis | - |
| 1.1.99.30 | additional information | anaerobic growth conditions | Proteus vulgaris | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.30 | 600 | - |
purified enzyme | Proteus mirabilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.30 | (R,S)-2-oxo-3-methylpentanoate + reduced benzyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | (R,S)-2-oxo-3-methylpentanoate + reduced methyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-methylpentanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | (S)-2-oxo-3-methylpentanoate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | (S)-2-oxo-3-methylpentanoate + reduced benzyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-methylpentanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | (S)-2-oxo-3-methylpentanoate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-methylpentanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | (S)-2-oxo-3-methylpentanoate + reduced methyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-methylpentanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced benzyl viologen | - |
Proteus mirabilis | 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced benzyl viologen | - |
Proteus vulgaris | 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced methyl viologen | - |
Proteus mirabilis | 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-3,3-dimethyl-4-hydroxybutanoate + reduced methyl viologen | - |
Proteus vulgaris | 3,3-dimethyl-2,4-dihydroxybutanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-4-(hydroxy-methyl-phosphinyl)-butanoate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxy-4-(hydroxymethylphosphinyl)-butanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-4-methylpentanoate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxy-4-methylpentanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxo-4-methylpentanoate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxy-4-methylpentanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoadipate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxyadipate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoadipate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxyadipate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoglutarate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxyglutarate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoglutarate + reduced benzyl viologen | - |
Proteus vulgaris | 2-hydroxyglutarate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoglutarate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxyglutarate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxoglutarate + reduced methyl viologen | - |
Proteus vulgaris | 2-hydroxyglutarate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 2-oxononanoate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxynonanoate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 2-oxononanoate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxynonanoate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 3-fluoropyruvate + reduced benzyl viologen | - |
Proteus mirabilis | 3-fluoro-2-hydroxypropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 3-fluoropyruvate + reduced benzyl viologen | - |
Proteus vulgaris | 3-fluoro-2-hydroxypropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 3-fluoropyruvate + reduced methyl viologen | - |
Proteus mirabilis | 3-fluoro-2-hydroxypropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 3-fluoropyruvate + reduced methyl viologen | - |
Proteus vulgaris | 3-fluoro-2-hydroxypropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | 5-benzyloxyindolylpyruvate + reduced benzyl viologen | - |
Proteus mirabilis | 3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | 5-benzyloxyindolylpyruvate + reduced methyl viologen | - |
Proteus mirabilis | 3-(5-benzyloxyindolyl)-2-hydroxypropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | a (2R)-hydroxy-carboxylate + acceptor | - |
Proteus sp. | a 2-oxo-carboxylate + reduced acceptor | - |
? | |
| 1.1.99.30 | indolylpyruvate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-indolylpropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | indolylpyruvate + reduced benzyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-indolylpropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | indolylpyruvate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-indolylpropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | indolylpyruvate + reduced methyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-indolylpropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | additional information | broad substrate specificity, stereochemic specificity, no activity with 3-oxoglutarate and hydroxyacetone | Proteus mirabilis | ? | - |
? | |
| 1.1.99.30 | additional information | broad substrate specificity, stereochemic specificity, no activity with 3-oxoglutarate and hydroxyacetone | Proteus vulgaris | ? | - |
? | |
| 1.1.99.30 | phenylglyoxylate + reduced benzyl viologen | - |
Proteus mirabilis | (R)-mandelic acid | - |
? | |
| 1.1.99.30 | phenylglyoxylate + reduced benzyl viologen | - |
Proteus vulgaris | (R)-mandelic acid | - |
? | |
| 1.1.99.30 | phenylglyoxylate + reduced methyl viologen | - |
Proteus mirabilis | (R)-mandelic acid | - |
? | |
| 1.1.99.30 | phenylglyoxylate + reduced methyl viologen | - |
Proteus vulgaris | (R)-mandelic acid | - |
? | |
| 1.1.99.30 | phenylpyruvate + reduced benzyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-phenylpropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | phenylpyruvate + reduced benzyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-phenylpropionate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | phenylpyruvate + reduced methyl viologen | - |
Proteus mirabilis | 2-hydroxy-3-phenylpropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | phenylpyruvate + reduced methyl viologen | - |
Proteus vulgaris | 2-hydroxy-3-phenylpropionate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | pyruvate + reduced benzyl viologen | - |
Proteus mirabilis | lactate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | pyruvate + reduced benzyl viologen | - |
Proteus vulgaris | lactate + oxidized benzyl viologen | - |
? | |
| 1.1.99.30 | pyruvate + reduced methyl viologen | - |
Proteus mirabilis | lactate + oxidized methyl viologen | - |
? | |
| 1.1.99.30 | pyruvate + reduced methyl viologen | - |
Proteus vulgaris | lactate + oxidized methyl viologen | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.30 | 37 | - |
assay at | Proteus mirabilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.30 | 22 | - |
room temperature, 30 h, stable | Proteus mirabilis |
| 1.1.99.30 | 22 | - |
room temperature, 30 h, stable | Proteus vulgaris |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.30 | additional information | non-pyridine nucleotide-dependent | Proteus mirabilis | |
| 1.1.99.30 | additional information | non-pyridine nucleotide-dependent | Proteus vulgaris |
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Release 2023.1 (January 2023)
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