Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garrad, R.C.; Bhattacharjee, J.K.
    Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans (1992), J. Bacteriol., 174, 7379-7384.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.87 medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Candida albicans
1.1.1.87 medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Cryptococcus neoformans
1.1.1.87 medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.87 LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Saccharomyces cerevisiae
-
 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Candida albicans
-
 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Cryptococcus neoformans
-
 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Aspergillus fumigatus
-
 2-oxoadipate + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.87 Aspergillus fumigatus
-
-
-
1.1.1.87 Candida albicans
-
-
-
1.1.1.87 Cryptococcus neoformans
-
-
-
1.1.1.87 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.87 additional information
-
 mutant 18804 1.4 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Candida albicans
1.1.1.87 additional information
-
 1.2 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Saccharomyces cerevisiae
1.1.1.87 additional information
-
 several mutant with about 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Cryptococcus neoformans
1.1.1.87 additional information
-
 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Saccharomyces cerevisiae 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Candida albicans 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Cryptococcus neoformans 2-oxoadipate + CO2 + NADH
-
 ?
1.1.1.87 1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Aspergillus fumigatus 2-oxoadipate + CO2 + NADH
-
 ?