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Literature summary extracted from
- Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini (1990), Arch. Microbiol., 154, 605-613.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.17.1.5 | lyophilization completely inactivates, high protein concentration may have a stabilizing effect on enzyme activity, sugar protects enzyme from dissociation | Neobacillus niacini |
| 1.17.3.3 | significant loss of activity during the purification procedure, lyophilization results in a complete loss of activity | Neobacillus niacini |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.5 | KCN | after removal of cyanide under anaerobic conditions, enzyme activity can be reconstituted by anaerobic incubation with Na2S | Neobacillus niacini |  |
| 1.17.1.5 | Sulfide | complete loss of activity without previous KCN-treatment | Neobacillus niacini | |
| 1.17.3.3 | glycerol | at a final concentration 10% (v/v), inactivates to 30% of the initial activity | Neobacillus niacini | |
| 1.17.3.3 | KCN | - |
Neobacillus niacini | |
| 1.17.3.3 | additional information | not inhibited by NaCl | Neobacillus niacini | |
| 1.17.3.3 | Na2S | partially purified 6-HDH, 90% loss of activity | Neobacillus niacini | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.5 | 1 | - |
nicotinate | - |
Neobacillus niacini | |
| 1.17.3.3 | 0.8 | - |
6-Hydroxynicotinate | pH 7.5, 30°C | Neobacillus niacini | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.17.1.5 | soluble | - |
Neobacillus niacini | - |
- |
| 1.17.3.3 | soluble | - |
Neobacillus niacini | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.3.3 | Iron | contains iron, iron-sulfur centres | Neobacillus niacini | |
| 1.17.3.3 | sulfur | contains acid-labile sulfur, iron-sulfur centres | Neobacillus niacini | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.5 | 20000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
| 1.17.1.5 | 34000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
| 1.17.1.5 | 85000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
| 1.17.1.5 | 280000 | 340000 | gel filtration, non-denaturing PAGE | Neobacillus niacini |
| 1.17.3.3 | 15000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 1.17.3.3 | 34000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 1.17.3.3 | 85000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 1.17.3.3 | 100000 | 140000 | non-denaturing PAGE, gel filtration | Neobacillus niacini |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.5 | nicotinate + H2O + electron acceptor | Neobacillus niacini | first step of nicotinate catabolism | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
| 1.17.3.3 | additional information | Neobacillus niacini | acts together with nicotinate dehydrogenase in nicotinate catabolism | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.5 | Neobacillus niacini | - |
DSM 2923 | - |
| 1.17.3.3 | Neobacillus niacini | - |
- |
- |
| 1.17.3.3 | Neobacillus niacini | - |
strain DSM 2923 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.5 | - |
Neobacillus niacini |
| 1.17.3.3 | - |
Neobacillus niacini |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.17.3.3 | 6-hydroxynicotinate + H2O + O2 = 2,6-dihydroxynicotinate + H2O2 | contains [2Fe-2S] iron-sulfur centres, FAD and molybdenum, it also has a catalytically essential, labile selenium that can be removed by reaction with cyanide, in Bacillus niacini, this enzyme is required for growth on nicotinic acid | Neobacillus niacini |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.5 | 0.54 | - |
- |
Neobacillus niacini |
| 1.17.3.3 | 0.7 | - |
pH 7.5, 30°C | Neobacillus niacini |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.17.1.5 | -20°C, increase of activity in crude extract | Neobacillus niacini |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.5 | additional information | no activity with Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD(P)+, and both viologens as electron acceptors, not as substrates: 2- or 6-aminonicotinamide, 2-aminonicotinic acid, chelidamic acid, cinchomeronic acid, citrazinic acid, dinicotinic acid, dipicolinic acid, 3-hydroxypicolinic acid, hypoxanthine, isocinchomeronic acid, isonicotinic acid, lutidinic acid, nicotinamide, xanthine | Neobacillus niacini | ? | - |
? | |
| 1.17.1.5 | nicotinate + H2O + electron acceptor | equilibrium lies far in direction of 6-hydroxynicotinate formation | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
r | |
| 1.17.1.5 | nicotinate + H2O + electron acceptor | high substrate specificity, transfers electrons only to artificial electron acceptors of high redox potential, i.e. thiazolyl blue, nitroblue tetrazolium chloride, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol, methylene blue with 40% of the activity with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
r | |
| 1.17.1.5 | nicotinate + H2O + electron acceptor | first step of nicotinate catabolism | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + 2,6-dichlorophenol indophenol | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced 2,6-dichlorophenol indophenol | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + methylene blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 40% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced methylene blue | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + nitroblue tetrazolium chloride | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced nitroblue tetrazolium chloride | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + O2 | - |
Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + O2 | highly specific for 6-hydroxynicotinate | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + phenazine ethosulfate | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced phenazine ethosulfate | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + thiazolyl blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thiazolyl blue | - |
? | |
| 1.17.3.3 | 6-hydroxynicotinate + H2O + thionin | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 2% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thionin | - |
? | |
| 1.17.3.3 | additional information | no electron acceptors: Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD+, NADP+, and both viologens | Neobacillus niacini | ? | - |
? | |
| 1.17.3.3 | additional information | acts together with nicotinate dehydrogenase in nicotinate catabolism | Neobacillus niacini | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.1.5 | oligomer | x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
| 1.17.3.3 | ? | x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.17.3.3 | 6-HDH | - |
Neobacillus niacini |
| 1.17.3.3 | 6-hydroxynicotinate hydroxylase | - |
Neobacillus niacini |
| 1.17.3.3 | 6-hydroxynicotinic acid dehydrogenase | - |
Neobacillus niacini |
| 1.17.3.3 | 6-hydroxynicotinic acid hydroxylase | - |
Neobacillus niacini |
| 1.17.3.3 | dehydrogenase, 6-hydroxynicotinate | - |
Neobacillus niacini |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.5 | 30 | - |
assay at | Neobacillus niacini |
| 1.17.3.3 | 30 | - |
assay at | Neobacillus niacini |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.5 | 7.5 | - |
- |
Neobacillus niacini |
| 1.17.3.3 | 7.5 | - |
- |
Neobacillus niacini |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.5 | 4Fe-4S-center | enzyme contains Fe/S centers, 8.3 mol iron per mol of enzyme, 1.5 mol acid-labile sulfur per mol of enzyme | Neobacillus niacini | |
| 1.17.1.5 | Bactopterin | enzyme contains bactopterin, an eubacterial modification of molybdopterin, the organic moiety of the molybdenum cofactor | Neobacillus niacini | |
| 1.17.1.5 | FAD | enzyme contains FAD, 2 mol flavin per mol of enzyme | Neobacillus niacini | |
| 1.17.1.5 | molybdenum cofactor | enzyme contains 1.5 mol molybdenum per mol of enzyme and bactopterin | Neobacillus niacini | |
| 1.17.3.3 | FAD | contains FAD | Neobacillus niacini | |
| 1.17.3.3 | iron-sulfur centre | contains iron-sulfur centres, iron and acid-labile sulfur | Neobacillus niacini | |
| 1.17.3.3 | molybdenum cofactor | contains molybdenum | Neobacillus niacini | |
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