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Literature summary extracted from

  • Gladyshev, V.N.; Khangulov, S.V.; Stadtman, T.C.
    Nicotinic acid hydroxylase from Clostridium barkeri: Electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme (1994), Proc. Natl. Acad. Sci. USA, 91, 232-236.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.5 23000
-
 1 * 50000 + 1 * 37000 + 1 * 33000 + 1 * 23000, SDS-PAGE Eubacterium barkeri
1.17.1.5 33000
-
 1 * 50000 + 1 * 37000 + 1 * 33000 + 1 * 23000, SDS-PAGE Eubacterium barkeri
1.17.1.5 37000
-
 1 * 50000 + 1 * 37000 + 1 * 33000 + 1 * 23000, SDS-PAGE Eubacterium barkeri
1.17.1.5 50000
-
 1 * 50000 + 1 * 37000 + 1 * 33000 + 1 * 23000, SDS-PAGE Eubacterium barkeri
1.17.1.5 160000
-
 native PAGE Eubacterium barkeri

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.5 Eubacterium barkeri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.5 oxygen-sensitive enzyme Eubacterium barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.5
-
 Eubacterium barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.5 additional information
-
 more values for specific activities in crude extract Eubacterium barkeri
1.17.1.5 4.4
-
 purified enzyme, Difco yeast extract without selenium as growth medium Eubacterium barkeri
1.17.1.5 20
-
 purified enzyme, average value, Difco yeast extract with selenium as growth medium Eubacterium barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.5 nicotinate + H2O + NADP+ NADP+ or various dyes as electron acceptors Eubacterium barkeri 6-hydroxynicotinate + NADPH with NADPH as substrate, enzyme exhibits NADPH oxidase activity in presence of oxygen r

Subunits

EC Number Subunits Comment Organism
1.17.1.5 heterotetramer 1 * 50000 + 1 * 37000 + 1 * 33000 + 1 * 23000, SDS-PAGE Eubacterium barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.5 4Fe-4S-center enzyme contains iron-sulfur clusters, [2Fe-2S] centers Eubacterium barkeri
1.17.1.5 FAD
-
 Eubacterium barkeri
1.17.1.5 molybdenum cofactor
-
 Eubacterium barkeri
1.17.1.5 molybdopterin molybdenum in the molybdopterin cofactor is coordinated directly with a dissociable selenium moiety, molybdenum center plays a role in the reaction step involving hydroxylation of the heterocyclic ring with oxygen derived from water Eubacterium barkeri
1.17.1.5 additional information enzyme contains a dissociable selenium moiety, which is coordinated directly with molybdenum in the molybdopterin cofactor, selenium is essential for enzyme activity, molar ratio of selenium to enzyme is 0.4-0.8, enzyme contains 1 g selenium per mol of native active enzyme, selenium is completely dissociated from protein in the SDS-gel Eubacterium barkeri
1.17.1.5 NADP+ NADP+ as ultimate electron acceptor, can be replaced by various dyes Eubacterium barkeri
1.17.1.5 NADPH NADPH oxidase activity in the presence of oxygen Eubacterium barkeri