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Literature summary extracted from

  • Nicholson, D.W.; Klemba, M.W.; Rasper, D.M.; Metters, K.M.; Zamboni, R.J.
    Purification of human leukotriene C4 synthase from dimethylsulfoxide-differentiated U937 cells (1992), Eur. J. Biochem., 209, 725-734.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.4.1.20 partially purified enzyme requires substrate stabilization for long-term storage Homo sapiens
4.4.1.20 reduced glutathione irreversibly inactivates the enzyme when present during freeze/thaw cycles and storage at concentrations above 5 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.20 Co2+
-
 Homo sapiens
4.4.1.20 KCl 0.5 mM, about 25% inhibition Homo sapiens
4.4.1.20 leukotriene C2 IC50: 0.0011 mM Homo sapiens
4.4.1.20 leukotriene C4 end product inhibition Homo sapiens
4.4.1.20 leukotriene D4
-
 Homo sapiens
4.4.1.20 leukotriene E4
-
 Homo sapiens
4.4.1.20 NaCl 0.5 mM, about 25% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.20 0.0166
-
 leukotriene A4
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.20 Ca2+ stimulates Homo sapiens
4.4.1.20 Mg2+ 30 mM, 1.8fold stimulation of partially purified enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.20
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.20 U-937 cell monocytic leukemia cell line Homo sapiens
-
4.4.1.20 U-937 cell dimethylsulfoxide-differentiated U937 cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.20 1.74
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.4.1.20 -80°C, in presence of 2-4 mM glutathione, stable for up to 1 year Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.20 leukotriene A4 + glutathione
-
 Homo sapiens leukotriene C4 + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.20 4
-
 t1/2: 18 h Homo sapiens
4.4.1.20 25
-
 t1/2: 18 h Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.20 0.00036
-
 leukotriene C2
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.4.1.20 0.0011
-
 IC50: 0.0011 mM Homo sapiens leukotriene C2