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Literature summary extracted from

  • Izumi, T.; Honda, Z.; Ohishi, N.; Kitamura, S.; Tsuchida, S.; Sato, K.; Shimizu, T.; Seyama, Y.
    Solubilization and partial purification of leukotriene C4 synthase from guinea-pig lung: a microsomal enzyme with high specificity towards 5,6-epoxide leukotriene A4 (1988), Biochim. Biophys. Acta, 959, 305-315.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.20 hexylglutathione IC50: 1.4 mM Cavia porcellus
4.4.1.20 hexylglutathione
-
 Rattus norvegicus
4.4.1.20 NEM IC50: 0.018 mM Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.20 0.02
-
 leukotriene A4 methyl ester pH 8.0, 37°C Cavia porcellus
4.4.1.20 0.036
-
 leukotriene A4 pH 8.0, 37°C Cavia porcellus
4.4.1.20 0.07
-
 14,15-Leukotriene A4 methyl ester pH 8.0, 37°C Cavia porcellus
4.4.1.20 0.13
-
 14,15-Leukotriene A4 pH 8.0, 37°C Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.20 microsome
-
 Cavia porcellus
-
-
4.4.1.20 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Cavia porcellus
-
-
-
4.4.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.20 partial Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.20 adrenal gland low activity Cavia porcellus
-
4.4.1.20 brain low activity Cavia porcellus
-
4.4.1.20 colon low activity Cavia porcellus
-
4.4.1.20 heart
-
 Cavia porcellus
-
4.4.1.20 kidney
-
 Cavia porcellus
-
4.4.1.20 kidney
-
 Rattus norvegicus
-
4.4.1.20 liver
-
 Rattus norvegicus
-
4.4.1.20 liver low activity Cavia porcellus
-
4.4.1.20 lung
-
 Cavia porcellus
-
4.4.1.20 lung
-
 Rattus norvegicus
-
4.4.1.20 small intestine low activity Cavia porcellus
-
4.4.1.20 spleen
-
 Cavia porcellus
-
4.4.1.20 stomach low activity Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.20 0.00786
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.20 14,15-leukotriene A4 + glutathione
-
 Cavia porcellus ?
-
 ?
4.4.1.20 14,15-leukotriene A4 methyl ester + glutathione
-
 Cavia porcellus ?
-
 ?
4.4.1.20 leukotriene A4 + glutathione
-
 Cavia porcellus leukotriene C4 + H2O
-
 ?
4.4.1.20 leukotriene A4 + glutathione
-
 Rattus norvegicus leukotriene C4 + H2O
-
 ?
4.4.1.20 leukotriene A4 methyl ester + glutathione
-
 Cavia porcellus leukotriene C4 methyl ester + H2O
-
 ?
4.4.1.20 leukotriene A4 methyl ester + glutathione
-
 Rattus norvegicus leukotriene C4 methyl ester + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.20 37
-
 3 min, 50 min loss of activity, 10 mM glutathione completely protects against inactivation Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.20 7.6 8.2
-
 Cavia porcellus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.4.1.20 0.018
-
 IC50: 0.018 mM Cavia porcellus NEM
4.4.1.20 1.4
-
 IC50: 1.4 mM Cavia porcellus hexylglutathione