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Literature summary extracted from

  • Maggio-Hall, L.A.; Escalante-Semerena, J.C.
    In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes (1999), Proc. Natl. Acad. Sci. USA, 96, 11798-11803.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.26 expression of His-tagged CobS in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.26 His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3) Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole Salmonella enterica subsp. enterica serovar Typhimurium
-
 GMP + adenosylcobalamin
-
 ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole Salmonella enterica subsp. enterica serovar Typhimurium de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin adenosylcobalamin + GMP
-
 ?
3.1.3.73 alpha-ribazole 5'-phosphate + H2O Salmonella enterica subsp. enterica serovar Typhimurium de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, last steps in adenosylcobalamin biosynthesis alpha-ribazole + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.26 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.8.26 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strains JE212 and JE2197
-
3.1.3.73 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strains JE212 and JE2197
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.26
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.26 nickel affinity chromatography, recombinant CobS Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.26 0.008
-
 recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium GMP + adenosylcobalamin
-
 ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobalamin + GMP
-
 ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobalamin + GMP
-
 ?
3.1.3.73 adenosylcobalamin 5'-phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobalamin + phosphate
-
 ?
3.1.3.73 alpha-ribazole 5'-phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium alpha-ribazole + phosphate
-
 ?
3.1.3.73 alpha-ribazole 5'-phosphate + H2O de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, last steps in adenosylcobalamin biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium alpha-ribazole + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.26 cobalamin synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.26 CobS
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.73 CobC
-
 Salmonella enterica subsp. enterica serovar Typhimurium