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Literature summary extracted from

  • Thompson, T.B.; Thomas, M.G.; Escalante-Semerena, J.C.; Rayment, I.
    Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site (1999), Biochemistry, 38, 12995-13005.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.156 X-ray crystal structure determined by molecular replacement to 2.2 A resolution, crystals grown by the batch method, cocrystallized with GTP and diphosphate, crystals belong to space group P2(1)2(1)2(1) with unit cell dimensions a : 58.4 A, b : 87.8 A, and c : 101.6 A Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 X-ray crystal structure determined by molecular replacement to 2.2 A resolution, crystals grown by the batch method, cocrystallized with GTP and diphosphate, crystals belong to space group P2(1)2(1)2(1) with unit cell dimensions a : 58.4 A, b : 87.8 A, and c : 101.6 A Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.156 diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.156 19000
-
 3 * 19000, homotrimer Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 19000
-
 3 * 19000, homotrimer Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.62 GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium biosynthesis of cobalamin, enzyme catalyzes the phosphorylation of the 1-amino-O-2-propanol side chain of the adenosylcobinamide ring diphosphate + adenosylcobinamide-GDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.156
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.62 GTP + adenosylcobinamide phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate biosynthesis of cobalamin, enzyme catalyzes the phosphorylation of the 1-amino-O-2-propanol side chain of the adenosylcobinamide ring Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 additional information CobU is a bifunctional enzyme that has both kinase EC 2.7.1.156 and guanyltransferase EC 2.7.7.62 activities Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.156 trimer 3 * 19000, homotrimer Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 trimer 3 * 19000, homotrimer Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.7.1.156 adenosylcobinamide kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 adenosylcobinamide phosphate guanyltransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium