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Literature summary extracted from

  • Tamaki, N.; Kaneko, M.; Mizota, C.; Kikugawa, M.; Fujimoto, S.
    Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver (1990), Eur. J. Biochem., 189, 39-45.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.40 6-Azauracil weak, kinetics, not reversible by pyridoxal phosphate Rattus norvegicus
2.6.1.40 6-azauridine weak Rattus norvegicus
2.6.1.40 Amino-oxyacetate strong Rattus norvegicus
2.6.1.40 D-cycloserine
-
 Rattus norvegicus
2.6.1.40 gabaculine
-
 Rattus norvegicus
2.6.1.40 additional information no inhibitor: dihydrothymine, dihydrouracil, N-carbamoyl-beta-alanine, N-carbamoyl-DL-3-aminoisobutyrate; no inhibitor: precursors of beta-alanine or (R)-3-amino-2-methylpropanoate, i.e. orotic acid, uracil, thymine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.40 0.45
-
 pyruvate pH 8.8, 37°C Rattus norvegicus
2.6.1.40 0.81
-
 beta-Alanine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.40 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.40 52000
-
 4 * 52000, SDS-PAGE Rattus norvegicus
2.6.1.40 220000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.40 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.40
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.40 liver
-
 Rattus norvegicus
-
2.6.1.40 additional information distribution in rat tissue Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.40 4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.40 (R)-3-amino-2-methylpropanoate + glyoxylate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + glycine
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + oxaloacetate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-aspartate
-
 ?
2.6.1.40 (R)-3-amino-2-methylpropanoate + pyruvate
-
 Rattus norvegicus 2-methyl-3-oxopropanoate + L-alanine
-
 ?
2.6.1.40 5-aminolevulinic acid + pyruvate
-
 Rattus norvegicus 5-oxolevulinic acid + L-alanine
-
 ?
2.6.1.40 beta-alanine + pyruvate
-
 Rattus norvegicus malonic semialdehyde + L-alanine
-
 ?
2.6.1.40 additional information overview Rattus norvegicus ?
-
 ?
2.6.1.40 additional information no substrate: 2-oxoglutarate Rattus norvegicus ?
-
 ?
2.6.1.40 additional information no substrate: phenylpyruvate Rattus norvegicus ?
-
 ?
2.6.1.40 NG,NG'-dimethyl-(S)-arginine + pyruvate
-
 Rattus norvegicus ? + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.40 tetramer 4 * 52000, SDS-PAGE Rattus norvegicus
2.6.1.40 tetramer cross-linking experiments: tetramer composed of two dimers Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.40 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.40 additional information
-
 pI: 6.7 Rattus norvegicus
2.6.1.40 9.5
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.40 pyridoxal 5'-phosphate
-
 Rattus norvegicus