BRENDA - Enzyme Database

Purification of ethanolaminephosphotransferase from bovine liver microsomes

Mancini, A.; Del Rosso, F.; Roberti, R.; Orvietani, P.; Coletti, L.; Binaglia, L.; Biochim. Biophys. Acta 1437, 80-92 (1999)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.00057
-
CDP-ethanolamine
37°C, pH 8.2
Bos taurus
2.7.8.1
0.00071
-
CDP-ethanolamine
cosubstrate CDP-choline, 37°C, pH 8.2
Bos taurus
2.7.8.1
0.04
-
CDP-choline
37°C, pH 8.2
Bos taurus
2.7.8.1
0.274
-
CDP-ethanolamine
cosubstrate CDP-choline, 37°C, pH 8.2
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mn2+
required
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.1
38000
-
x * 38000, SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Bos taurus
-
dual-specificity enzyme, activity of EC 2.7.8.1 and EC 2.7.8.2
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.1
shows activities of both EC 2.7.8.1 and EC 2.7.8.2
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-choline + 1,2-diacylglycerol
-
644414
Bos taurus
CMP + phosphatidylcholine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644414
Bos taurus
CMP + a phosphatidylethanolamine
-
644414
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.1
?
x * 38000, SDS-PAGE
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.00057
-
CDP-ethanolamine
37°C, pH 8.2
Bos taurus
2.7.8.1
0.00071
-
CDP-ethanolamine
cosubstrate CDP-choline, 37°C, pH 8.2
Bos taurus
2.7.8.1
0.04
-
CDP-choline
37°C, pH 8.2
Bos taurus
2.7.8.1
0.274
-
CDP-ethanolamine
cosubstrate CDP-choline, 37°C, pH 8.2
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mn2+
required
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.1
38000
-
x * 38000, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.1
shows activities of both EC 2.7.8.1 and EC 2.7.8.2
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-choline + 1,2-diacylglycerol
-
644414
Bos taurus
CMP + phosphatidylcholine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644414
Bos taurus
CMP + a phosphatidylethanolamine
-
644414
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.1
?
x * 38000, SDS-PAGE
Bos taurus