BRENDA - Enzyme Database

An improved procedure for the purification of ethanolaminephosphotransferase. Reconstitution of the purified enzyme with lipids

Roberti, R.; Vecchini, A.; Freysz, L.; Masoom, M.; Binaglia, L.; Biochim. Biophys. Acta 1004, 80-88 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
2.7.8.1
Dipalmitoylphosphatidylethanolamine
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
Km for CDP-ethanolamine depends on composition of the lipid mixture utilized for reconstitution of solubilized enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.1
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.1
1.705
-
37░C, pH 8.2
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644409
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644409
Rattus norvegicus
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.7.8.1
37
-
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
dipalmitoylphosphatidylcholine
-
Rattus norvegicus
2.7.8.1
Dipalmitoylphosphatidylethanolamine
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
Km for CDP-ethanolamine depends on composition of the lipid mixture utilized for reconstitution of solubilized enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.1
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.1
1.705
-
37░C, pH 8.2
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644409
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644409
Rattus norvegicus
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.7.8.1
37
-
-
Rattus norvegicus