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Literature summary extracted from

  • Henneberry, A.L.; McMaster, C.R.
    Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine (1999), Biochem. J., 339, 291-298.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.1
-
 Homo sapiens
2.7.8.2
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.1 Mn2+ above 10 mM Homo sapiens
2.7.8.2 Mn2+ inhibitory above 10 mM, activation below Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.1 0.037
-
 CDP-choline cosubstrate di-18:0 diacylglycerol, 20°C, pH 8.0 Homo sapiens
2.7.8.1 0.101
-
 CDP-ethanolamine cosubstrate di-18:0 diacylglycerol, 20°C, pH 8.0 Homo sapiens
2.7.8.2 0.037
-
 CDP-choline cosubstrate di-18:1-diacylglycerol, 20°C, pH 8.0 Homo sapiens
2.7.8.2 0.101
-
 CDP-ethanolamine cosubstrate di-18:1-diacylglycerol, 20°C, pH 8.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.1 Mg2+ activates, more effective on cholinephosphotransferase activity Homo sapiens
2.7.8.1 Mn2+ activates, more effective on ethanolaminephosphotransferase activity, inhibitory above 10 mM Homo sapiens
2.7.8.2 Mg2+ activates, acts mostly on cholinephosphotransferase activity Homo sapiens
2.7.8.2 Mn2+ activates, acts mostly on ethanolaminephosphotransferase activity, inhibitory above 10 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.1 46500
-
 x * 46500, SDS-PAGE and deduced from gene sequence Homo sapiens
2.7.8.2 46500
-
 x * 46500, hCEPT1, SDS-PAGE and deduced from gene sequence, seven membrane-spanning helices Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.1 Homo sapiens
-
 dual-specificity enzyme, activity of EC 2.7.8.1 and EC 2.7.8.2
-
2.7.8.2 Homo sapiens
-
 dual-specificity enzyme, activitiy of EC 2.7.8.1 and EC 2.7.8.2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.1 CDP-choline + 1,2-diacylglycerol preferred substrates in decreasing order: di-10:0 diacylglycerol, di-16:1 diacylglycerol, di-8:0 diacylglycerol, di-18:1 diacylglycerol, 16:0/22:6 diacylglycerol Homo sapiens CMP + phosphatidylcholine
-
 ?
2.7.8.1 CDP-ethanolamine + 1,2-diacylglycerol preferred substrates in decreasing order: di-18:1 diacylglycerol, di-16:1 diacylglycerol, 16:0/18:1 diacylglycerol, 16:0/22:6 diacylglycerol Homo sapiens CMP + a phosphatidylethanolamine
-
 ?
2.7.8.2 CDP-choline + 1,2-diacylglycerol preferred substrates in decreasing order: di-10:0 diacylglycerol, di-16:1 diacylglycerol, di-8:0 diacylglycerol, di-18:1 diacylglycerol, 16:0/22:6 diacylglycerol Homo sapiens CMP + a phosphatidylcholine
-
 ?
2.7.8.2 CDP-ethanolamine + 1,2-diacylglycerol preferred substrates in decreasing order: di-18:1 diacylglycerol, di-16:1 diacylglycerol, 16:0/18:1 diacylglycerol, 16:0/22:6 diacylglycerol Homo sapiens CMP + phosphatidylethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.1 ? x * 46500, SDS-PAGE and deduced from gene sequence Homo sapiens
2.7.8.2 ? x * 46500, hCEPT1, SDS-PAGE and deduced from gene sequence, seven membrane-spanning helices Homo sapiens