BRENDA - Enzyme Database

Solubilization and purification of rat liver microsomal 1,2-diacylglycerol: CDP-choline cholinephosphotransferase and 1,2-diacylglycerol: CDP-ethanolamine ethanolaminephosphotransferase

Kanoh, H.; Ohno, K.; Eur. J. Biochem. 66, 201-210 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
deoxycholate
activates
Rattus norvegicus
2.7.8.1
Triton X-100
slightly stimulates with Mn2+, but not with Mg2+ as cofactor
Rattus norvegicus
2.7.8.2
deoxycholate
stimulates
Rattus norvegicus
2.7.8.2
Phospholipids
microsomal phospholipids required for maximal activity
Rattus norvegicus
Application
EC Number
Application
Commentary
Organism
2.7.8.2
medicine
exposure of cortical neurons to neurotoxic concentrations of N-methyl-D-aspartate strongly reduces enzymic activity
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.7.8.1
Triton X-100, stable to
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
CDP-choline
-
Rattus norvegicus
2.7.8.1
ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetracetic acid
-
Rattus norvegicus
2.7.8.1
Microsomal phospholipids
-
Rattus norvegicus
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
Triton X-100
X-100
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Co2+
negligible effect
Rattus norvegicus
2.7.8.1
Mg2+
can partially replace Mn2+ in activation
Rattus norvegicus
2.7.8.1
Mn2+
much more effective than Mg2+
Rattus norvegicus
2.7.8.2
Mg2+
enzyme separated into Mg2+-requiring and Mn2+-requiring components; required
Rattus norvegicus
2.7.8.2
Mn2+
enzyme separated into Mg2+-requiring and Mn2+-requiring components
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
2.7.8.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.1
partial
Rattus norvegicus
2.7.8.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.1
12.1
-
30°C, pH 8.5
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644363
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644363
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644363
Rattus norvegicus
CMP + a phosphatidylcholine
-
644363
Rattus norvegicus
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
8.5
-
Rattus norvegicus
2.7.8.2
8
8.5
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
deoxycholate
activates
Rattus norvegicus
2.7.8.1
Triton X-100
slightly stimulates with Mn2+, but not with Mg2+ as cofactor
Rattus norvegicus
2.7.8.2
deoxycholate
stimulates
Rattus norvegicus
2.7.8.2
Phospholipids
microsomal phospholipids required for maximal activity
Rattus norvegicus
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.8.2
medicine
exposure of cortical neurons to neurotoxic concentrations of N-methyl-D-aspartate strongly reduces enzymic activity
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.8.1
Triton X-100, stable to
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
CDP-choline
-
Rattus norvegicus
2.7.8.1
ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetracetic acid
-
Rattus norvegicus
2.7.8.1
Microsomal phospholipids
-
Rattus norvegicus
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
Triton X-100
X-100
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Co2+
negligible effect
Rattus norvegicus
2.7.8.1
Mg2+
can partially replace Mn2+ in activation
Rattus norvegicus
2.7.8.1
Mn2+
much more effective than Mg2+
Rattus norvegicus
2.7.8.2
Mg2+
enzyme separated into Mg2+-requiring and Mn2+-requiring components; required
Rattus norvegicus
2.7.8.2
Mn2+
enzyme separated into Mg2+-requiring and Mn2+-requiring components
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.1
partial
Rattus norvegicus
2.7.8.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.1
12.1
-
30°C, pH 8.5
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644363
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644363
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644363
Rattus norvegicus
CMP + a phosphatidylcholine
-
644363
Rattus norvegicus
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
8.5
-
Rattus norvegicus
2.7.8.2
8
8.5
-
Rattus norvegicus