BRENDA - Enzyme Database

Acyl chain length dependency of diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase. Effect of different saturated fatty acids at the C-1 or C-2 position of diacylglycerol on solubilized rat liver microsomal enzymes

Morimoto, K.; Kanoh, H.; J. Biol. Chem. 253, 5056-5060 (1978)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
-
Rattus norvegicus
2.7.8.1
0.111
-
1-dodecanoyl-2-octadecenoylglycerol
i.e. 1-C16:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.114
-
1-pentadecanoyl-2-octadecenoylglycerol
i.e. 1-C15:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.12
-
1-heptadecanoyl-2-octadecenoylglycerol
i.e. 1-C17:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.128
-
1-nonadecanoyl-2-octadecenoylglycerol
i.e. 1-C19:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.147
-
1,2-dioleoylglycerol
i.e. 1,2-diC18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.167
-
1-octadecanoyl-2-octadecenoylglycerol
i.e. 1-C18:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.182
-
1-arachidoyl-2-octadecenoylglycerol
i.e. 1-C20:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
0.104
-
1-myristoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.109
-
1-pentadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.116
-
1-palmitoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.12
-
1,2-dioleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.122
-
1-tridecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.125
0.126
1-stearoyl-2-oleoyl-sn-glycerol
pH 8.0, 30°C
Rattus norvegicus
2.7.8.2
0.133
-
1-lauroyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.16
-
1-arachidoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.17
-
1-heptadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.21
-
1-nonadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
2.7.8.2
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644362
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644362
Rattus norvegicus
-
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
-
644362
Rattus norvegicus
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-heptadecanoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-lauroylglycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-lauroylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-palmitoylglycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-palmitoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-stearoyl-sn-glycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-stearoyl-sn-glycerol-3-phosphorylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-stearoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
CDP-ethanolamine + 2-oleoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
additional information
substrate specificity overview
644362
Rattus norvegicus
?
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644362
Rattus norvegicus
CMP + a phosphatidylcholine
-
644362
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-dioleoyl-sn-glycerol
-
644362
Rattus norvegicus
CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
-
-
2.7.8.2
additional information
specificity overview
644362
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
influence of saturated fatty acids on the enzyme activities depends on their location at the C-1 or C-2 position of glycerol
644362
Rattus norvegicus
?
-
-
-
-
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
additional information
-
additional information
-
Rattus norvegicus
2.7.8.1
0.111
-
1-dodecanoyl-2-octadecenoylglycerol
i.e. 1-C16:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.114
-
1-pentadecanoyl-2-octadecenoylglycerol
i.e. 1-C15:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.12
-
1-heptadecanoyl-2-octadecenoylglycerol
i.e. 1-C17:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.128
-
1-nonadecanoyl-2-octadecenoylglycerol
i.e. 1-C19:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.147
-
1,2-dioleoylglycerol
i.e. 1,2-diC18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.167
-
1-octadecanoyl-2-octadecenoylglycerol
i.e. 1-C18:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.182
-
1-arachidoyl-2-octadecenoylglycerol
i.e. 1-C20:0,2-C18:1glycerol, 30°C, pH 8.0
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
0.104
-
1-myristoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.109
-
1-pentadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.116
-
1-palmitoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.12
-
1,2-dioleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.122
-
1-tridecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.125
0.126
1-stearoyl-2-oleoyl-sn-glycerol
pH 8.0, 30°C
Rattus norvegicus
2.7.8.2
0.133
-
1-lauroyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.16
-
1-arachidoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.17
-
1-heptadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
0.21
-
1-nonadecanoyl-2-oleoyl-sn-glycerol
30°C, pH 8.0
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644362
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644362
Rattus norvegicus
-
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
-
644362
Rattus norvegicus
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-heptadecanoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-lauroylglycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-lauroylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-palmitoylglycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-palmitoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-oleoyl-2-stearoyl-sn-glycerol
-
644362
Rattus norvegicus
CMP + 1-oleoyl-2-stearoyl-sn-glycerol-3-phosphorylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-stearoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
CDP-ethanolamine + 2-oleoylglycerol
-
644362
Rattus norvegicus
CMP + ?
-
-
-
?
2.7.8.1
additional information
substrate specificity overview
644362
Rattus norvegicus
?
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644362
Rattus norvegicus
CMP + a phosphatidylcholine
-
644362
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-dioleoyl-sn-glycerol
-
644362
Rattus norvegicus
CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
-
-
2.7.8.2
additional information
specificity overview
644362
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
influence of saturated fatty acids on the enzyme activities depends on their location at the C-1 or C-2 position of glycerol
644362
Rattus norvegicus
?
-
-
-
-