BRENDA - Enzyme Database

Control of phosphatidylethanolamine metabolism in yeast: diacylglycerol ethanolaminephosphotransferase and diacylglycerol cholinephosphotransferase are separate enzymes

Percy, A.K.; Carson, M.A.; Moore, J.F.; Waechter, C.J.; Arch. Biochem. Biophys. 230, 69-81 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
1,2-diacylglycerol
stimulated by exogenous 1,2-diacylglycerols, largest stimulation by 1,2-diolein and 1,2-diacylglycerol
Saccharomyces cerevisiae
2.7.8.2
1,2-Dilaurin
-
Saccharomyces cerevisiae
2.7.8.2
1,2-diolein
highest stimulation of diacylglycerols tested
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
1,2-Dilaurin
slight
Saccharomyces cerevisiae
2.7.8.1
5'-AMP
slight
Saccharomyces cerevisiae
2.7.8.1
5'-CMP
-
Saccharomyces cerevisiae
2.7.8.1
5'-GMP
slight
Saccharomyces cerevisiae
2.7.8.1
5'-UMP
slight
Saccharomyces cerevisiae
2.7.8.1
CDP
-
Saccharomyces cerevisiae
2.7.8.1
CMP
-
Saccharomyces cerevisiae
2.7.8.1
CTP
-
Saccharomyces cerevisiae
2.7.8.1
DH-990
hypolidemic drug
Saccharomyces cerevisiae
2.7.8.1
p-hydroxymercuribenzoate
reversal by monothioglycerol
Saccharomyces cerevisiae
2.7.8.2
5'-AMP
little effect
Saccharomyces cerevisiae
2.7.8.2
5'-CTP
-
Saccharomyces cerevisiae
2.7.8.2
5'-GMP
little effect
Saccharomyces cerevisiae
2.7.8.2
5'-UMP
little effect
Saccharomyces cerevisiae
2.7.8.2
CDP
-
Saccharomyces cerevisiae
2.7.8.2
CMP
-
Saccharomyces cerevisiae
2.7.8.2
DH-990
hypolidemic drug
Saccharomyces cerevisiae
2.7.8.2
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.083
-
CDP-ethanolamine
37°C, pH 8.0
Saccharomyces cerevisiae
2.7.8.2
additional information
-
additional information
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mn2+
required
Saccharomyces cerevisiae
2.7.8.2
Mn2+
2-5 mM; required
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Saccharomyces cerevisiae
-
-
-
2.7.8.2
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
in presence of 5'-CMP
644361
Saccharomyces cerevisiae
CMP + a phosphatidylethanolamine
-
644361
Saccharomyces cerevisiae
r
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
-
644361
Saccharomyces cerevisiae
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644361
Saccharomyces cerevisiae
CMP + a phosphatidylcholine
-
644361
Saccharomyces cerevisiae
r
2.7.8.2
CMP + phosphatidylcholine
-
644361
Saccharomyces cerevisiae
CDP-choline + 1,2-diacylglycerol
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
30
37
-
Saccharomyces cerevisiae
2.7.8.2
30
45
-
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.1
50
-
t1/2: 8 min
Saccharomyces cerevisiae
2.7.8.2
50
-
t1/2: 1 min
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
-
-
Saccharomyces cerevisiae
2.7.8.2
7
-
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
1,2-diacylglycerol
stimulated by exogenous 1,2-diacylglycerols, largest stimulation by 1,2-diolein and 1,2-diacylglycerol
Saccharomyces cerevisiae
2.7.8.2
1,2-Dilaurin
-
Saccharomyces cerevisiae
2.7.8.2
1,2-diolein
highest stimulation of diacylglycerols tested
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
1,2-Dilaurin
slight
Saccharomyces cerevisiae
2.7.8.1
5'-AMP
slight
Saccharomyces cerevisiae
2.7.8.1
5'-CMP
-
Saccharomyces cerevisiae
2.7.8.1
5'-GMP
slight
Saccharomyces cerevisiae
2.7.8.1
5'-UMP
slight
Saccharomyces cerevisiae
2.7.8.1
CDP
-
Saccharomyces cerevisiae
2.7.8.1
CMP
-
Saccharomyces cerevisiae
2.7.8.1
CTP
-
Saccharomyces cerevisiae
2.7.8.1
DH-990
hypolidemic drug
Saccharomyces cerevisiae
2.7.8.1
p-hydroxymercuribenzoate
reversal by monothioglycerol
Saccharomyces cerevisiae
2.7.8.2
5'-AMP
little effect
Saccharomyces cerevisiae
2.7.8.2
5'-CTP
-
Saccharomyces cerevisiae
2.7.8.2
5'-GMP
little effect
Saccharomyces cerevisiae
2.7.8.2
5'-UMP
little effect
Saccharomyces cerevisiae
2.7.8.2
CDP
-
Saccharomyces cerevisiae
2.7.8.2
CMP
-
Saccharomyces cerevisiae
2.7.8.2
DH-990
hypolidemic drug
Saccharomyces cerevisiae
2.7.8.2
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.083
-
CDP-ethanolamine
37°C, pH 8.0
Saccharomyces cerevisiae
2.7.8.2
additional information
-
additional information
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mn2+
required
Saccharomyces cerevisiae
2.7.8.2
Mn2+
2-5 mM; required
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
in presence of 5'-CMP
644361
Saccharomyces cerevisiae
CMP + a phosphatidylethanolamine
-
644361
Saccharomyces cerevisiae
r
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
-
644361
Saccharomyces cerevisiae
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644361
Saccharomyces cerevisiae
CMP + a phosphatidylcholine
-
644361
Saccharomyces cerevisiae
r
2.7.8.2
CMP + phosphatidylcholine
-
644361
Saccharomyces cerevisiae
CDP-choline + 1,2-diacylglycerol
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
30
37
-
Saccharomyces cerevisiae
2.7.8.2
30
45
-
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.1
50
-
t1/2: 8 min
Saccharomyces cerevisiae
2.7.8.2
50
-
t1/2: 1 min
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
-
-
Saccharomyces cerevisiae
2.7.8.2
7
-
-
Saccharomyces cerevisiae