BRENDA - Enzyme Database

Reversibility of the reactions catalyzed by cholinephosphotransferase and ethanolaminephosphotransferase solubilized from rat-brain microsomes

Roberti, R.; Mancini, A.; Freysz, L.; Binaglia, L.; Biochim. Biophys. Acta 1165, 183-188 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
additional information
the rate of incorporation of CMP into CDP-ethanolamine is increased by increasing the concentration of phosphatidylethanolamine in detergent-phospholipid micellar system
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.0016
-
CDP-ethanolamine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
Mg2+ is less effective than Mn2+ as cofactor for the reverse reaction; Mn2+ or Mg2+ required
Rattus norvegicus
2.7.8.1
Mn2+
Mg2+ is less effective than Mn2+ as cofactor for the reverse reaction; Mn2+ or Mg2+ required; required
Rattus norvegicus
2.7.8.2
Mg2+
absolute requirement for Mg2+ or Mn2+; Mg2+ is less effective than Mn2+ as cofactor in the reverse reaction
Rattus norvegicus
2.7.8.2
Mn2+
absolute requirement for Mg2+ or Mn2+; Mg2+ is less effective than Mn2+ as cofactor in the reverse reaction
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
2.7.8.2
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
brain
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644355
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644355
Rattus norvegicus
r
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644355
Rattus norvegicus
CMP + a phosphatidylcholine
-
644355
Rattus norvegicus
r
2.7.8.2
CMP + phosphatidylcholine
-
644355
Rattus norvegicus
CDP-choline + 1,2-diacylglycerol
-
-
-
r
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
additional information
the rate of incorporation of CMP into CDP-ethanolamine is increased by increasing the concentration of phosphatidylethanolamine in detergent-phospholipid micellar system
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.0016
-
CDP-ethanolamine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
Mg2+ is less effective than Mn2+ as cofactor for the reverse reaction; Mn2+ or Mg2+ required
Rattus norvegicus
2.7.8.1
Mn2+
Mg2+ is less effective than Mn2+ as cofactor for the reverse reaction; Mn2+ or Mg2+ required; required
Rattus norvegicus
2.7.8.2
Mg2+
absolute requirement for Mg2+ or Mn2+; Mg2+ is less effective than Mn2+ as cofactor in the reverse reaction
Rattus norvegicus
2.7.8.2
Mn2+
absolute requirement for Mg2+ or Mn2+; Mg2+ is less effective than Mn2+ as cofactor in the reverse reaction
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
brain
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644355
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644355
Rattus norvegicus
r
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644355
Rattus norvegicus
CMP + a phosphatidylcholine
-
644355
Rattus norvegicus
r
2.7.8.2
CMP + phosphatidylcholine
-
644355
Rattus norvegicus
CDP-choline + 1,2-diacylglycerol
-
-
-
r