BRENDA - Enzyme Database

Hamster liver cholinephosphotransferase and ethanolaminephosphotransferase are separate enzymes

O, K.M.; Siow, Y.L.; Choy, P.C.; Biochem. Cell Biol. 67, 680-686 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
CHAPS
activates
Mesocricetus auratus
2.7.8.1
octyl glucoside
activates at low concentration, inhibits at higher concentration
Mesocricetus auratus
2.7.8.1
taurocholate
-
Mesocricetus auratus
2.7.8.2
Phospholipids
phosphatidylcholine, phosphatidylethanolamine or lysophosphatidylethanolamine activates, lysophosphatidylcholine inhibits
Mesocricetus auratus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
octyl glucoside
activates at low concentration, inhibits at higher concentration
Mesocricetus auratus
2.7.8.2
CHAPS
-
Mesocricetus auratus
2.7.8.2
CHAPSO
-
Mesocricetus auratus
2.7.8.2
n-octylglucoside
-
Mesocricetus auratus
2.7.8.2
Phospholipids
phosphatidylcholine, phosphatidylethanolamine or lysophosphatidylethanolamine activates, lysophosphatidylcholine inhibits
Mesocricetus auratus
2.7.8.2
Triton X-100
X-200
Mesocricetus auratus
2.7.8.2
Tween-20
-
Mesocricetus auratus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Mesocricetus auratus
-
-
2.7.8.2
microsome
-
Mesocricetus auratus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
can partially replace Mn2+ in activation; Mn2+ or Mg2+ required
Mesocricetus auratus
2.7.8.1
Mn2+
Mn2+ or Mg2+ required; much more effective than Mg2+
Mesocricetus auratus
2.7.8.2
Mg2+
absolute requirement for Mg2+ or Mn2+; maximal activation at 10 mM
Mesocricetus auratus
2.7.8.2
Mn2+
absolute requirement for Mg2+ or Mn2+
Mesocricetus auratus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
Mesocricetus auratus
-
CMP + a phosphatidylethanolamine
-
-
r
2.7.8.1
additional information
Mesocricetus auratus
catalyzes the final step in synthesis of phosphatidylethanolamine
?
-
-
-
2.7.8.2
CDP-choline + sn-1,2-diacylglycerol
Mesocricetus auratus
final reaction in synthesis of phosphatidylcholine
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Mesocricetus auratus
-
-
-
2.7.8.2
Mesocricetus auratus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.1
partial
Mesocricetus auratus
2.7.8.2
partial
Mesocricetus auratus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Mesocricetus auratus
-
2.7.8.2
liver
-
Mesocricetus auratus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylethanolamine
-
-
-
r
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylethanolamine
-
644354
Mesocricetus auratus
r
2.7.8.1
lysophosphatidylcholine + CMP
75% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
r
2.7.8.1
lysophosphatidylethanolamine + CMP
74% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
r
2.7.8.1
additional information
catalyzes the final step in synthesis of phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
-
2.7.8.1
phosphatidylcholine + CMP
88% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
CDP-choline + diacylglycerol
-
-
-
r
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylcholine
-
644354
Mesocricetus auratus
?
2.7.8.2
CDP-choline + sn-1,2-diacylglycerol
final reaction in synthesis of phosphatidylcholine
644354
Mesocricetus auratus
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
37
-
-
Mesocricetus auratus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.1
55
-
1 min, 90% loss of activity
Mesocricetus auratus
2.7.8.2
55
-
1 min, 90% loss of activity, whole microsomes, less than 60% loss of activity, partially purified enzyme
Mesocricetus auratus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8.5
-
broad
Mesocricetus auratus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
CHAPS
activates
Mesocricetus auratus
2.7.8.1
octyl glucoside
activates at low concentration, inhibits at higher concentration
Mesocricetus auratus
2.7.8.1
taurocholate
-
Mesocricetus auratus
2.7.8.2
Phospholipids
phosphatidylcholine, phosphatidylethanolamine or lysophosphatidylethanolamine activates, lysophosphatidylcholine inhibits
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
octyl glucoside
activates at low concentration, inhibits at higher concentration
Mesocricetus auratus
2.7.8.2
CHAPS
-
Mesocricetus auratus
2.7.8.2
CHAPSO
-
Mesocricetus auratus
2.7.8.2
n-octylglucoside
-
Mesocricetus auratus
2.7.8.2
Phospholipids
phosphatidylcholine, phosphatidylethanolamine or lysophosphatidylethanolamine activates, lysophosphatidylcholine inhibits
Mesocricetus auratus
2.7.8.2
Triton X-100
X-200
Mesocricetus auratus
2.7.8.2
Tween-20
-
Mesocricetus auratus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Mesocricetus auratus
-
-
2.7.8.2
microsome
-
Mesocricetus auratus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
can partially replace Mn2+ in activation; Mn2+ or Mg2+ required
Mesocricetus auratus
2.7.8.1
Mn2+
Mn2+ or Mg2+ required; much more effective than Mg2+
Mesocricetus auratus
2.7.8.2
Mg2+
absolute requirement for Mg2+ or Mn2+; maximal activation at 10 mM
Mesocricetus auratus
2.7.8.2
Mn2+
absolute requirement for Mg2+ or Mn2+
Mesocricetus auratus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
Mesocricetus auratus
-
CMP + a phosphatidylethanolamine
-
-
r
2.7.8.1
additional information
Mesocricetus auratus
catalyzes the final step in synthesis of phosphatidylethanolamine
?
-
-
-
2.7.8.2
CDP-choline + sn-1,2-diacylglycerol
Mesocricetus auratus
final reaction in synthesis of phosphatidylcholine
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.1
partial
Mesocricetus auratus
2.7.8.2
partial
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Mesocricetus auratus
-
2.7.8.2
liver
-
Mesocricetus auratus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylethanolamine
-
-
-
r
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylethanolamine
-
644354
Mesocricetus auratus
r
2.7.8.1
lysophosphatidylcholine + CMP
75% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
r
2.7.8.1
lysophosphatidylethanolamine + CMP
74% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
r
2.7.8.1
additional information
catalyzes the final step in synthesis of phosphatidylethanolamine
644354
Mesocricetus auratus
?
-
-
-
-
2.7.8.1
phosphatidylcholine + CMP
88% of the activity with phosphatidylethanolamine
644354
Mesocricetus auratus
CDP-choline + diacylglycerol
-
-
-
r
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644354
Mesocricetus auratus
CMP + a phosphatidylcholine
-
644354
Mesocricetus auratus
?
2.7.8.2
CDP-choline + sn-1,2-diacylglycerol
final reaction in synthesis of phosphatidylcholine
644354
Mesocricetus auratus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
37
-
-
Mesocricetus auratus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.1
55
-
1 min, 90% loss of activity
Mesocricetus auratus
2.7.8.2
55
-
1 min, 90% loss of activity, whole microsomes, less than 60% loss of activity, partially purified enzyme
Mesocricetus auratus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8.5
-
broad
Mesocricetus auratus