EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.2 | deoxycholate | stimulates, inhibition above 2 mM | Rattus norvegicus | |
2.7.8.2 | phosphatidylcholine | activates | Rattus norvegicus | |
2.7.8.2 | Phospholipids | total microsomal phospholipids stimulate | Rattus norvegicus | |
2.7.8.2 | taurocholate | stimulates | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.7.8.1 | stable if treated with 0.5% Triton X-100 | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.2 | CDPethanolamine | - |
Rattus norvegicus | |
2.7.8.2 | CMPcholine | product inhibition | Rattus norvegicus | |
2.7.8.2 | deoxycholate | stimulates, inhibition above 2 mM | Rattus norvegicus | |
2.7.8.2 | Triton X-100 | activity assayed with Mg2+ is more labile than that assayed with Mn2+; X-100 | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.1 | 0.022 | - |
CDP-ethanolamine | 30°C, pH 8.0 | Rattus norvegicus | |
2.7.8.1 | 0.063 | - |
diacylglycerol | 30°C, pH 8.0 | Rattus norvegicus | |
2.7.8.1 | 0.14 | - |
CMP | 30°C, pH 8.0 | Rattus norvegicus | |
2.7.8.2 | additional information | - |
additional information | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.1 | microsome | cytoplasmic surface | Rattus norvegicus | - |
- |
2.7.8.2 | microsome | cytoplasmic face of microsomal vesicles | Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.1 | Mg2+ | can partially replace Mn2+ in activation | Rattus norvegicus | |
2.7.8.1 | Mn2+ | required | Rattus norvegicus | |
2.7.8.1 | Mn2+ | much more effective than Mg2+ | Rattus norvegicus | |
2.7.8.2 | Mg2+ | required | Rattus norvegicus | |
2.7.8.2 | Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.1 | Rattus norvegicus | - |
- |
- |
2.7.8.2 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.1 | - |
Rattus norvegicus |
2.7.8.2 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.8.1 | liver | - |
Rattus norvegicus | - |
2.7.8.2 | brain | - |
Rattus norvegicus | - |
2.7.8.2 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.8.1 | additional information | - |
- |
Rattus norvegicus |
2.7.8.2 | additional information | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.8.1 | below -20°C, stable for more than 1 month, microsomal preparation | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.1 | CDP-ethanolamine + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylethanolamine | - |
r | |
2.7.8.1 | additional information | several 1-oleoyl-2-saturated types of diacylglycerol are effectively used | Rattus norvegicus | ? | - |
? | |
2.7.8.1 | additional information | fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized | Rattus norvegicus | ? | - |
? | |
2.7.8.1 | additional information | 1-acyl-2-oleoylglycerols ranging from 1-lauroyl to 1-heptadecanoyl species: 1-heptadecanoyl and 1-stearoyl species used most actively | Rattus norvegicus | ? | - |
? | |
2.7.8.1 | phosphatidylcholine + CMP | - |
Rattus norvegicus | CDP-choline + diacylglycerol | - |
? | |
2.7.8.2 | CDP-choline + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylcholine | - |
r | |
2.7.8.2 | CDP-choline + 1,2-dipalmitoyl-sn-glycerol | poor substrate | Rattus norvegicus | CMP + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine | - |
? | |
2.7.8.2 | CMP + phosphatidylcholine | - |
Rattus norvegicus | CDP-choline + 1,2-diacylglycerol | - |
r | |
2.7.8.2 | additional information | 1-acyl-2-oleoylglycerols, ranging from 1-lauroyl to 1-heptadecanoyl species are equally well utilized, fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized, several 1-oleoyl-2-saturated types of diacylglycerol are effectively used | Rattus norvegicus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.1 | additional information | - |
temperature dependence | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.1 | 8 | 8.5 | - |
Rattus norvegicus |
2.7.8.2 | 8 | 8.5 | - |
Rattus norvegicus |