BRENDA - Enzyme Database

1,2-Diacylglycerol: CDPcholine cholinephosphotransferase

Kanoh, H.; Ohno, K.; Methods Enzymol. 71, 536-546 (1981)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.2
deoxycholate
stimulates, inhibition above 2 mM
Rattus norvegicus
2.7.8.2
phosphatidylcholine
activates
Rattus norvegicus
2.7.8.2
Phospholipids
total microsomal phospholipids stimulate
Rattus norvegicus
2.7.8.2
taurocholate
stimulates
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.7.8.1
stable if treated with 0.5% Triton X-100
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
CMPcholine
product inhibition
Rattus norvegicus
2.7.8.2
deoxycholate
stimulates, inhibition above 2 mM
Rattus norvegicus
2.7.8.2
Triton X-100
activity assayed with Mg2+ is more labile than that assayed with Mn2+; X-100
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.022
-
CDP-ethanolamine
30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.063
-
diacylglycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.14
-
CMP
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
cytoplasmic surface
Rattus norvegicus
-
-
2.7.8.2
microsome
cytoplasmic face of microsomal vesicles
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
can partially replace Mn2+ in activation
Rattus norvegicus
2.7.8.1
Mn2+
much more effective than Mg2+; required
Rattus norvegicus
2.7.8.2
Mg2+
required
Rattus norvegicus
2.7.8.2
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
2.7.8.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.1
-
Rattus norvegicus
2.7.8.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.1
additional information
-
-
Rattus norvegicus
2.7.8.2
additional information
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.7.8.1
below -20°C, stable for more than 1 month, microsomal preparation
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644343
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644343
Rattus norvegicus
r
2.7.8.1
additional information
several 1-oleoyl-2-saturated types of diacylglycerol are effectively used
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
additional information
fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
additional information
1-acyl-2-oleoylglycerols ranging from 1-lauroyl to 1-heptadecanoyl species: 1-heptadecanoyl and 1-stearoyl species used most actively
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
phosphatidylcholine + CMP
-
644343
Rattus norvegicus
CDP-choline + diacylglycerol
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644343
Rattus norvegicus
CMP + a phosphatidylcholine
-
644343
Rattus norvegicus
r
2.7.8.2
CDP-choline + 1,2-dipalmitoyl-sn-glycerol
poor substrate
644343
Rattus norvegicus
CMP + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
-
-
?
2.7.8.2
CMP + phosphatidylcholine
-
644343
Rattus norvegicus
CDP-choline + 1,2-diacylglycerol
-
-
-
r
2.7.8.2
additional information
1-acyl-2-oleoylglycerols, ranging from 1-lauroyl to 1-heptadecanoyl species are equally well utilized, fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized, several 1-oleoyl-2-saturated types of diacylglycerol are effectively used
644343
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
additional information
-
temperature dependence
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
8.5
-
Rattus norvegicus
2.7.8.2
8
8.5
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.2
deoxycholate
stimulates, inhibition above 2 mM
Rattus norvegicus
2.7.8.2
phosphatidylcholine
activates
Rattus norvegicus
2.7.8.2
Phospholipids
total microsomal phospholipids stimulate
Rattus norvegicus
2.7.8.2
taurocholate
stimulates
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.8.1
stable if treated with 0.5% Triton X-100
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
CMPcholine
product inhibition
Rattus norvegicus
2.7.8.2
deoxycholate
stimulates, inhibition above 2 mM
Rattus norvegicus
2.7.8.2
Triton X-100
activity assayed with Mg2+ is more labile than that assayed with Mn2+; X-100
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.022
-
CDP-ethanolamine
30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.063
-
diacylglycerol
30°C, pH 8.0
Rattus norvegicus
2.7.8.1
0.14
-
CMP
30°C, pH 8.0
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
cytoplasmic surface
Rattus norvegicus
-
-
2.7.8.2
microsome
cytoplasmic face of microsomal vesicles
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
can partially replace Mn2+ in activation
Rattus norvegicus
2.7.8.1
Mn2+
much more effective than Mg2+; required
Rattus norvegicus
2.7.8.2
Mg2+
required
Rattus norvegicus
2.7.8.2
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.1
-
Rattus norvegicus
2.7.8.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.1
additional information
-
-
Rattus norvegicus
2.7.8.2
additional information
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.8.1
below -20°C, stable for more than 1 month, microsomal preparation
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644343
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644343
Rattus norvegicus
r
2.7.8.1
additional information
several 1-oleoyl-2-saturated types of diacylglycerol are effectively used
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
additional information
fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
additional information
1-acyl-2-oleoylglycerols ranging from 1-lauroyl to 1-heptadecanoyl species: 1-heptadecanoyl and 1-stearoyl species used most actively
644343
Rattus norvegicus
?
-
-
-
-
2.7.8.1
phosphatidylcholine + CMP
-
644343
Rattus norvegicus
CDP-choline + diacylglycerol
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644343
Rattus norvegicus
CMP + a phosphatidylcholine
-
644343
Rattus norvegicus
r
2.7.8.2
CDP-choline + 1,2-dipalmitoyl-sn-glycerol
poor substrate
644343
Rattus norvegicus
CMP + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
-
-
?
2.7.8.2
CMP + phosphatidylcholine
-
644343
Rattus norvegicus
CDP-choline + 1,2-diacylglycerol
-
-
-
r
2.7.8.2
additional information
1-acyl-2-oleoylglycerols, ranging from 1-lauroyl to 1-heptadecanoyl species are equally well utilized, fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized, several 1-oleoyl-2-saturated types of diacylglycerol are effectively used
644343
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.1
additional information
-
temperature dependence
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8
8.5
-
Rattus norvegicus
2.7.8.2
8
8.5
-
Rattus norvegicus