BRENDA - Enzyme Database

Phospholipid synthesis in isolated fat cells. Studies of microsomal diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase activities

Coleman, R.; Bell, R.M.; J. Biol. Chem. 252, 3050-3056 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
bovine serum albumin
1 mg/ml, 2fold increase of activity
Rattus norvegicus
2.7.8.1
EGTA
stimulates
Rattus norvegicus
2.7.8.1
pospholipase C
10 min, 23C, 26% stimulation
Rattus norvegicus
2.7.8.2
EGTA
0.5 mM, stimulates
Rattus norvegicus
2.7.8.2
ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid
stimulates
Rattus norvegicus
2.7.8.2
Phospholipase C
23C, 10 min, slight stimulation
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.7.8.1
trypsin, 70%, 0.9 min, activity remains stable
Rattus norvegicus
2.7.8.2
freezing and thawing the microsomal preparation 5times destroys activity by 50%
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
acetone
-
Rattus norvegicus
2.7.8.1
Ca2+
CaCl2
Rattus norvegicus
2.7.8.1
CDP
-
Rattus norvegicus
2.7.8.1
CDP-choline
-
Rattus norvegicus
2.7.8.1
CMP
-
Rattus norvegicus
2.7.8.1
CTP
-
Rattus norvegicus
2.7.8.1
dioxane
-
Rattus norvegicus
2.7.8.1
dithiothreitol
-
Rattus norvegicus
2.7.8.1
methanol
-
Rattus norvegicus
2.7.8.1
palmitoyl-CoA
-
Rattus norvegicus
2.7.8.1
Triton WR 1339
-
Rattus norvegicus
2.7.8.1
Tween 20
-
Rattus norvegicus
2.7.8.2
1-butanol
-
Rattus norvegicus
2.7.8.2
acetone
-
Rattus norvegicus
2.7.8.2
Ca2+
-
Rattus norvegicus
2.7.8.2
CDP
-
Rattus norvegicus
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
dithiothreitol
-
Rattus norvegicus
2.7.8.2
methanol
-
Rattus norvegicus
2.7.8.2
palmitoyl-CoA
-
Rattus norvegicus
2.7.8.2
Triton X-100
WR 1339; X-100
Rattus norvegicus
2.7.8.2
Trypsin
-
Rattus norvegicus
2.7.8.2
Tween-20
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.0118
-
1,2-dioleoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.1
0.0183
-
CDP-ethanolamine
cosubstrate 1,2-dioleoyl-sn-glycerol
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
0.0057
-
didodecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0141
-
didecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0239
-
CDP-choline
cosubstrate 1,2-dioleoyl-sn-glycerol, 23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0314
-
dihexadecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0405
-
bacterial diacylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0501
-
dioctadecenoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0509
-
1,2-dioleoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0681
-
dihexanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.118
-
dimyristoyl-sn-glycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.125
0.126
1-stearoyl-2-oleoyl-sn-glycerol
23C, pH 8.5
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
completely dependent on MgCl2; Mn2+ or Mg2+ required
Rattus norvegicus
2.7.8.1
Mn2+
Mn2+ or Mg2+ required; much more effective than Mg2+
Rattus norvegicus
2.7.8.2
Mg2+
required
Rattus norvegicus
2.7.8.2
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.1
Rattus norvegicus
-
-
-
2.7.8.2
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
adipocyte
-
Rattus norvegicus
-
2.7.8.1
brain
-
Rattus norvegicus
-
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.1
mucosa
of intestine
Rattus norvegicus
-
2.7.8.2
adipocyte
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
2.7.8.2
lung
fetal
Rattus norvegicus
-
2.7.8.2
mucosa
of intestine
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644323
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644323
Rattus norvegicus
-
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
highest activity
644323
Rattus norvegicus
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-stearoyl-2-oleoylglycerol
-
644323
Rattus norvegicus
CMP + 1-stearoyl-2-oleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
additional information
1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length are no substrates
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644323
Rattus norvegicus
CMP + a phosphatidylcholine
-
644323
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-dioleoyl-sn-glycerol
-
644323
Rattus norvegicus
CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
-
?
2.7.8.2
CDP-choline + didecanoylglycerol
-
644323
Rattus norvegicus
CMP + didecanoylglycerophosphocholine
-
-
-
-
2.7.8.2
CDP-choline + dihexanoylglycerol
-
644323
Rattus norvegicus
CMP + dihexanoylglycerophosphocholine
-
-
-
-
2.7.8.2
additional information
specificity overview
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
no substrate: 1,3-diC18:1-glycerol ether
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
no substrate: 1,2-diC18:1-glycerol ether
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
substrates are 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length
644323
Rattus norvegicus
?
-
-
-
-
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.8.1
37
-
stable for 19 min, heating of microsomes
Rattus norvegicus
2.7.8.1
49
-
4 min, more than 50% loss of activity
Rattus norvegicus
2.7.8.2
37
-
19 min, stable, microsomal preparation
Rattus norvegicus
2.7.8.2
49
-
15 min, 50% loss of activity, microsomal preparation
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8.5
9.3
-
Rattus norvegicus
2.7.8.2
8.5
9.3
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.1
bovine serum albumin
1 mg/ml, 2fold increase of activity
Rattus norvegicus
2.7.8.1
EGTA
stimulates
Rattus norvegicus
2.7.8.1
pospholipase C
10 min, 23C, 26% stimulation
Rattus norvegicus
2.7.8.2
EGTA
0.5 mM, stimulates
Rattus norvegicus
2.7.8.2
ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid
stimulates
Rattus norvegicus
2.7.8.2
Phospholipase C
23C, 10 min, slight stimulation
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.8.1
trypsin, 70%, 0.9 min, activity remains stable
Rattus norvegicus
2.7.8.2
freezing and thawing the microsomal preparation 5times destroys activity by 50%
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.1
acetone
-
Rattus norvegicus
2.7.8.1
Ca2+
CaCl2
Rattus norvegicus
2.7.8.1
CDP
-
Rattus norvegicus
2.7.8.1
CDP-choline
-
Rattus norvegicus
2.7.8.1
CMP
-
Rattus norvegicus
2.7.8.1
CTP
-
Rattus norvegicus
2.7.8.1
dioxane
-
Rattus norvegicus
2.7.8.1
dithiothreitol
-
Rattus norvegicus
2.7.8.1
methanol
-
Rattus norvegicus
2.7.8.1
palmitoyl-CoA
-
Rattus norvegicus
2.7.8.1
Triton WR 1339
-
Rattus norvegicus
2.7.8.1
Tween 20
-
Rattus norvegicus
2.7.8.2
1-butanol
-
Rattus norvegicus
2.7.8.2
acetone
-
Rattus norvegicus
2.7.8.2
Ca2+
-
Rattus norvegicus
2.7.8.2
CDP
-
Rattus norvegicus
2.7.8.2
CDPethanolamine
-
Rattus norvegicus
2.7.8.2
dithiothreitol
-
Rattus norvegicus
2.7.8.2
methanol
-
Rattus norvegicus
2.7.8.2
palmitoyl-CoA
-
Rattus norvegicus
2.7.8.2
Triton X-100
WR 1339; X-100
Rattus norvegicus
2.7.8.2
Trypsin
-
Rattus norvegicus
2.7.8.2
Tween-20
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.1
0.0118
-
1,2-dioleoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.1
0.0183
-
CDP-ethanolamine
cosubstrate 1,2-dioleoyl-sn-glycerol
Rattus norvegicus
2.7.8.2
additional information
-
additional information
-
Rattus norvegicus
2.7.8.2
0.0057
-
didodecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0141
-
didecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0239
-
CDP-choline
cosubstrate 1,2-dioleoyl-sn-glycerol, 23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0314
-
dihexadecanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0405
-
bacterial diacylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0501
-
dioctadecenoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0509
-
1,2-dioleoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.0681
-
dihexanoylglycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.118
-
dimyristoyl-sn-glycerol
23C, pH 8.5
Rattus norvegicus
2.7.8.2
0.125
0.126
1-stearoyl-2-oleoyl-sn-glycerol
23C, pH 8.5
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.1
microsome
-
Rattus norvegicus
-
-
2.7.8.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.1
Mg2+
completely dependent on MgCl2; Mn2+ or Mg2+ required
Rattus norvegicus
2.7.8.1
Mn2+
Mn2+ or Mg2+ required; much more effective than Mg2+
Rattus norvegicus
2.7.8.2
Mg2+
required
Rattus norvegicus
2.7.8.2
Mn2+
can partially replace Mg2+ in activation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.1
adipocyte
-
Rattus norvegicus
-
2.7.8.1
brain
-
Rattus norvegicus
-
2.7.8.1
liver
-
Rattus norvegicus
-
2.7.8.1
mucosa
of intestine
Rattus norvegicus
-
2.7.8.2
adipocyte
-
Rattus norvegicus
-
2.7.8.2
brain
-
Rattus norvegicus
-
2.7.8.2
liver
-
Rattus norvegicus
-
2.7.8.2
lung
fetal
Rattus norvegicus
-
2.7.8.2
mucosa
of intestine
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.1
CDP-ethanolamine + 1,2-diacylglycerol
-
644323
Rattus norvegicus
CMP + a phosphatidylethanolamine
-
644323
Rattus norvegicus
-
2.7.8.1
CDP-ethanolamine + 1,2-dioleoylglycerol
highest activity
644323
Rattus norvegicus
CMP + dioleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
CDP-ethanolamine + 1-stearoyl-2-oleoylglycerol
-
644323
Rattus norvegicus
CMP + 1-stearoyl-2-oleoylphosphatidylethanolamine
-
-
-
?
2.7.8.1
additional information
1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length are no substrates
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
CDP-choline + 1,2-diacylglycerol
-
644323
Rattus norvegicus
CMP + a phosphatidylcholine
-
644323
Rattus norvegicus
-
2.7.8.2
CDP-choline + 1,2-dioleoyl-sn-glycerol
-
644323
Rattus norvegicus
CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
-
?
2.7.8.2
CDP-choline + didecanoylglycerol
-
644323
Rattus norvegicus
CMP + didecanoylglycerophosphocholine
-
-
-
-
2.7.8.2
CDP-choline + dihexanoylglycerol
-
644323
Rattus norvegicus
CMP + dihexanoylglycerophosphocholine
-
-
-
-
2.7.8.2
additional information
specificity overview
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
no substrate: 1,3-diC18:1-glycerol ether
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
no substrate: 1,2-diC18:1-glycerol ether
644323
Rattus norvegicus
?
-
-
-
-
2.7.8.2
additional information
substrates are 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length
644323
Rattus norvegicus
?
-
-
-
-
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.8.1
37
-
stable for 19 min, heating of microsomes
Rattus norvegicus
2.7.8.1
49
-
4 min, more than 50% loss of activity
Rattus norvegicus
2.7.8.2
37
-
19 min, stable, microsomal preparation
Rattus norvegicus
2.7.8.2
49
-
15 min, 50% loss of activity, microsomal preparation
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.1
8.5
9.3
-
Rattus norvegicus
2.7.8.2
8.5
9.3
-
Rattus norvegicus