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Literature summary extracted from

  • Ladrat, C.; Verrez-Bagnis, V.; Noel, J.; Fleurence, J.
    Milli-calpain from sea bass (Dicentrarchus labrax) white muscle: purification, characterization of its activity and activation in vitro (2002), Mar. Biotechnol., 4, 51-62.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 (2S,3S)-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester 0.01 mM, 50% inhibition Dicentrarchus labrax
3.4.22.53 3,4-dichloroisocoumarin 0.05 mM, 11% inhibition Dicentrarchus labrax
3.4.22.53 4-[[(3,4-dinitrophenyl)carbonyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid 5 mM, 23% inhibition Dicentrarchus labrax
3.4.22.53 alpha2-Macroglobulin 0.05 mg/ml, 11% inhibition Dicentrarchus labrax
3.4.22.53 antipain 0.05 mM, complete inhibition Dicentrarchus labrax
3.4.22.53 calpain inhibitor I 0.05 mM, 99% inhibition Dicentrarchus labrax
3.4.22.53 calpain inhibitor II 0.05 mM, complete inhibition Dicentrarchus labrax
3.4.22.53 Co2+ 2.5 mM, strong Dicentrarchus labrax
3.4.22.53 E-64 0.01 mM, 98% inhibition Dicentrarchus labrax
3.4.22.53 EDTA 5 mM, complete inhibition Dicentrarchus labrax
3.4.22.53 Fe2+ 2.5 mM, strong Dicentrarchus labrax
3.4.22.53 Fe3+ 2.5 mM, complete Dicentrarchus labrax
3.4.22.53 Hg2+ 2.5 mM, complete Dicentrarchus labrax
3.4.22.53 iodoacetic acid 0.1 mM, 99% inhibition Dicentrarchus labrax
3.4.22.53 leupeptin 0.05 mM, 99% inhibition Dicentrarchus labrax
3.4.22.53 Mn2+ 2.5 mM, strong Dicentrarchus labrax
3.4.22.53 N-tosyl-Lys-chloromethyl ketone 0.5 mM, 69% inhibition Dicentrarchus labrax
3.4.22.53 N-tosyl-Phe-chloromethyl ketone 0.5 mM, 91% inhibition Dicentrarchus labrax
3.4.22.53 NEM 5 mM, complete inhibition Dicentrarchus labrax
3.4.22.53 Ni2+ 2.5 mM, complete Dicentrarchus labrax
3.4.22.53 PCMB 5 mM, 24% inhibition Dicentrarchus labrax
3.4.22.53 pepstatin 0.01 mM, 99% inhibition Dicentrarchus labrax
3.4.22.53 PMSF 1 mM, 6% inhibition Dicentrarchus labrax
3.4.22.53 protease inhibitor 0.002 mg/ml, 6% inhibition Dicentrarchus labrax
3.4.22.53 Zn2+ 2.5 mM, complete Dicentrarchus labrax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.53 0.431
-
 succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Dicentrarchus labrax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ba2+ activates Dicentrarchus labrax
3.4.22.53 Ca2+ best activator at 2.5 mM, maximal caseinolytic activity at 2.2 mM, half-maximal caseinolytic activity at 0.312 mM Dicentrarchus labrax
3.4.22.53 Cu2+ activates Dicentrarchus labrax
3.4.22.53 Mn2+ activates Dicentrarchus labrax
3.4.22.53 Sr2+ 2.5 mM, 66% of the activation obtained with Ca2+, maximal caseinolytic activity at 5.9 mM, half-maximal caseinolytic activity at 1.886 mM. Autolysis in presence of 5 mM Ca2+. The 80000 Da subunit is rapidly autolyzed in two smaller bands of 73000 Da and 69000 Da. The small subunit of 24000 da is degraded into three bands of 22000 Da, 19300 Da and 17800 Da. It is not clear whether autolysis is necessary for calpain to become proteolytically active Dicentrarchus labrax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.53 24000
-
 1 * 80000 + 1 * 24000, SDS-PAGE Dicentrarchus labrax
3.4.22.53 80000
-
 1 * 80000 + 1 * 24000, SDS-PAGE Dicentrarchus labrax
3.4.22.53 124000
-
 gel filtration Dicentrarchus labrax

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Dicentrarchus labrax
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.53 additional information the enzyme is neither glycosylated nor phosphorylated Dicentrarchus labrax

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.53
-
 Dicentrarchus labrax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.53 white muscle present in very low amounts, only 0.0033% Dicentrarchus labrax
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.53 casein + H2O
-
 Dicentrarchus labrax ?
-
 ?
3.4.22.53 succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Dicentrarchus labrax ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.53 dimer 1 * 80000 + 1 * 24000, SDS-PAGE Dicentrarchus labrax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.53 24
-
-
 Dicentrarchus labrax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.53 7
-
-
 Dicentrarchus labrax